Search results for " Biochimica"

Improvement of the expression of upper limb muscle strength and balance in a group of patients with multiple sclerosis, through a proprioceptive reha…

2022

In multiple sclerosis (MS) patients, symptoms such as: fatigue, lack of physical energy, spasticity, difficulty in performing movements, and motor coordination disorders, and with tremors, dizziness and postural instability are among the most common complications. Cattaneo et al. (2007) studied the effects of stability training on MS patients and described it as an effective intervention in reducing falls risk, improving stability, and strength. Therefore, the present study aimed to confirm early observations by the application of a proprioceptive rehabilitation program targeting balance and strength on MS patients. 13 subjects with MS, 5 male and 8 female, volunteered in the study. A MAP h…

The implication of MBL deficient haplotypes in acute coronary syndrome

2014

Rat PIPPin is probably part of a large complex of RNA-binding proteins

2012

Throughout rat brain development, expression of histones variants is mainly regulated at the post-transcriptional level. We previously cloned two cDNAs encoding, respectively, PIPPin (or CSD-C2), a brain-enriched protein able to bind the 3’end of H1° and H3.3 mRNAs, and LPI (longer isoform of PEP-19). Both PEP-19 and LPI are brain-specific. By western blot, we found that PIPPin expression in PC12 cells is enhanced by NGF-induced differentiation. We investigated the RNA-binding properties of the three proteins using their 6 histidine-tagged recombinant fusions and found that they all bind H1° and H3.3 RNAs. Since PEP-19 and LPI are camstatins, we also analyzed whether calmodulin could interf…

Role of RNA-binding proteins in the replication-independent expression of H1° and H3.3 histone variants

2012

GSH: A MARKER FOR OXIDATIVE STRESS IN HUMAN CELL CULTURES

2013

GSH: A MARKER FOR OXIDATIVE STRESS IN HUMAN CELL CULTURES Gueli Maria Concetta Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche (BioNEC), Università degli Studi di Palermo. Reactive oxygen species (ROS) play an important role in physiological processes, but when being in excess ROS react readily with proteins, lipids, carbohydrates, and nucleic acids, often inducing irreversible functional alterations or even complete destruction. In cells, under physiological conditions, the production and detoxification of ROS are more or less balanced. GSH functions as antioxidant and the oxidative conversion of GSH to GSSG is widely recognized as a reliable index of oxidative stress. The…

In vitro models of blood-brain barrier and application in the study of the multiple sclerosis

2008

Sviluppo di nanocarriers per il “drug release” controllato di biomolecole nella terapia antitumorale

2014

Una nuova generazione di nanogels (NGs) in poly(N-vinyl pyrrolidone) (PVP) è stata ottenuta mediante beta–irraggiamento (e-beam) ad alta energia mediante elettroni accelerati, che permette in tempi brevi la reticolazione di polimeri in soluzione acquosa, utilizzando acceleratori di particelle lineari. Oltre al controllo dimensionale, l’aspetto innovativo di tale tecnica è la possibilità di ottenere nanogels sterili, la cui funzionalizzazione di superficie avviene in un unico step. Inizialmente, è stata provata la loro biocompatibilità e non genotossicità in sistemi biologici, caratteristiche fondamentali per la loro applicazione in campo biomedico. Inoltre, sono stati messi a punto dei prot…

Towards the standardization of mitochondrial proteomics: the Italian mt-HPP initiative

2017

The mitochondrial Human Proteome Project aims at understanding the function of the mitochondrial proteome and its crosstalk with the proteome of other organelles. Being able to choose a suitable and validated enrichment protocol of functional mitochondria, based on the specific needs of the downstream proteomics analysis, would greatly help the researchers in the field. Mitochondrial fractions from ten model cell lines were prepared using three enrichment protocols and analyzed on seven different LC-MS/MS platforms. All data were processed using neXtProt as reference database. The data are available for the Human Proteome Project purposes through the ProteomeXchange Consortium with the iden…

INFLUENCE OF NUTRITION AND GENETICS ON PERFORMANCE: A PILOT STUDY IN A GROUP OF GYMNASTS

2017

Purpose. Energy requirements in sports performance are affected by numerous factors: physical characteristics, age, genetic basis, sports discipline. The Food and Nutrition Board recommended nutrition based on age, height, and body weight gain in relation to physical activity. Some genetic factors, such as the PPAR-encoding gene, play a key role in metabolism. The aim of the study was to investigate the effect of specific snacks on performance. Methods. Seventeen girls aged 10–14 years practising artistic gymnastics were enrolled. A carb or protein/carb snack was provided before the training, and a questionnaire was administered at the end. In addition, saliva samples were collected for gen…

Shed vesicles are involved in the release of some leaderless proteins.

2006

Most proteins destined for secretion in the extracellular matrix are characterized by the presence of N-terminal signal peptides which direct their translocation into the endoplasmic reticulum, they are subsequently transferred to the Golgi apparatus and then secreted in the extracellular space. A growing number of secreted proteins, are being identified which, however, lack signal peptides allowing their entrance into the endoplasmic reticulum. They include the inflammatory cytokine interleukin 1b, galactins, macrophage migration inhibitory factor (MIF), acid and basic fibroblast growth factors (FGF-1, FGF-2) and Sphingosine kinase1(SphK-1). These proteins are secreted from the cell by unc…