Search results for " Biodeterioration"
showing 9 items of 19 documents
FRESHWATER CYANOBACTERIA, IDENTIFIED BY MICROSCOPIC AND MOLECULAR INVESTIGATIONS ON A COLONIZED FOUNTAIN SURFACE: A CASE STUDY IN PALERMO (SICILY, IT…
2021
Cyanobacteria or blue algae are ubiquitously present in both fresh and brackish water environments. They also grow in conditions of high humidity, colonizing stones or monuments and fountain surfaces, and creating thick biofilms able to induce biodeterioration in the constituent materials of artefacts. As well as several photoautotrophic organisms, cyanobacteria belong to the microorganisms identified as primary colonizers, playing an important role in stone artwork deterioration. In this study, an analysis was made of the biofilm collected from the stone fountain of the Two Dragons in Palermo (Italy), revealing the presence of cyanobacterial colonies by optical microscopy, due to their pec…
Bacteria consortia and deterioration of archaeological waterlogged wood: identification by molecular and microscopy techniques
2014
In this study molecular tools are applied to reveal and identify bacterial colonization in waterlogged wood to assessing the changes induced in anatomical structure, previously observed by Optical and Scanning Electron Microscopy (1). The results obtained by observation of wooden thin sections (OM), shown the presence of black and dark-brown areas and mineral concretions. The SEM analysis revealed a specific cell walls alteration, attributable to bacterial activity, other than abundant pyrite framboids (FeS2). The presence of sulfur compounds in archaeological waterlogged wood can indicate both long-term burial in anoxic environment and colonization by sulfate-reducing bacteria. Molecular m…
ANTHROPOMORPHIC WOODEN RELIQUARIES FROM KNOWLEDGE TO RESTORATION
2014
Two wood anthropomorphic reliquaries, belonging to the Lipsanoteca of Epiphany Church in Trapani (Italy) were restored following the consevative restoration procedures based on an interdisciplinary approach. The hystoric-artistic contextualization, the constitutive materials and executive techniques were defined and physical-chemical-biological factors involved in degradation events were evaluated. The Saints relic (human bone or teeth), present in each reliquary were analysed through visual investigation. After restoration, the manufacts showed their peculiarities and their extraordinary cultural value.
La flora briofitica dell’area archeologica di Selinunte (Sicilia sud-occidentale).
2018
The bryophyte flora settled on the ancient ruins and other habitats of the archaeological area of Selinunte has been studied. The study showed that the bryophyte flora consists of 51 specific and infraspecific taxa (44 mosses and 7 liverworts). The analysis points out that the bryophyte flora of Selinunte is the richest from the taxonomic point of view. moreover, it is characterized by a prevalence of mediterranean taxa and has a distinctly photophilous, thermophilous, xerophilous and calcicolous-neutrophilous connotation. Biodeteriogenic species potentially more dangerous for their spread, abundance and reproductive capacity are highlighted.
Identification of microbial taxa involved in cultural heritage deterioration and able to produce health hazardous substances by molecular techniques.
2008
Fungi and bacterial, wide-spread in biosphere environments, are the main microorganisms related to the deterioration of cultural assets but, moreover, complex microbial communities may emit mixed aerosol into indoor air. In this study the microbial colonization is investigated from to point of view, conservation of cultural heritage and related potential illness to visitors or professionals. The sampling was performed by non-destructive procedures on works of art surfaces, and by the gelatin membrane filter method (Sartorius) for aerosol. The identification of microbial taxa was performed by molecular analyses based on PCR, sequencing, sequence comparison techniques and, particularly for fu…
Monitoring Biological Damage on Paper-based Documents in the Historical Archive of the Palermo Astronomical Observatory
2017
Libraries and archives house a wide variety of documents made of materials of vegetal and animal origin: unbound papers and books, manuscripts and printed books, photographs (negative and positive), prints, maps, available to the public for reading needs, study and information. These materials are often subject to fluctuations in environmental and micro-environmental parameters. For this reason, it is essential to implement constant monitoring and control of environmental conditions and potential deteriogens in order to slow down deterioration processes. The monitoring of the microbial degradation of paper documents in the Historical Archives of Palermo Astronomical Observatory has revealed…
IDENTIFICATION OF BACTERIAL TAXA IN ARCHAEOLOGICAL WATERLOGGED WOOD
2014
Summary The microscopic and molecular techniques described in this study are aimed at understanding the degradation processes of the anatomical structure of submerged archaeological wood, correlating it to the degradation induced by bacteria. The SEM micrographs showed alterations of the wooden structure due to bacterial colonization, as well as the presence of pyrite framboids. The difficulty of extracting bacterial DNA from wooden fragments belonging to submerged finds is well-known, due to the presence of many inhibitors; this study describes some extraction and in vitro amplification protocols for wooden submerged finds. The results of the molecular investigations, based on the analysis…
Rivelazione e Caratterizzazione di Consorzi Microbici in Reperti Lignei Sommersi
2009
In questa lavoro sono state affrontate le problematiche relative alla valutazione dello stato di conservazione del legno archeologico sommerso (waterlogged wood) in relazione ai fattori che ne inducono il degrado. L’attenzione è stata rivolta ai processi di biodeterioramento, indotti dall’attività di alcuni microrganismi che utilizzano le componenti principali del legno, la cui identificazione è stata eseguita ricorrendo a tecniche sia colturali sia molecolari. In particolare, le tecniche molecolari che si sono rivelate di immediata applicazione per lo studio e la caratterizzazione dei microrganismi che colonizzano i beni di interesse storico artistico e i manufatti d’interesse archeologico…
Plant essential oils in controlling fungal colonization on wooden substrate
2021
In order to control fungal colonization and the related deterioration process of cultural objects, several chemical compounds are actually utilised. These products generally are toxic, not biodegradable and persisting for long time in the environment, also acting on not-targeted biological systems. In this study, specifically to wooden cultural object, Origanum vulgare L. and Thymus vulgaris L. essential oils are proposed as green biocides to contrast the development of Penicillium chrysogenum, Aspergillus flavus and Aspergillus niger fungal species. The aim is replacing toxic products with natural molecules, proposing alternative methodologies supporting the using of non-toxic novel compou…