Search results for " Cell"

Entry and processing of Borrelia burgdorferi pleomorphic forms

2015

Lymen borrelioosi on Suomessakin yleinen puutiaisten levittämä tauti. Borrelioosin ensimmäisiä oireita ovat muun muassa puutiaisen pureman ympärille muodostuva ihottuma, päänsärky, väsymys ja kuume. Myöhemmin tauti voi aiheuttaa nivel- tai sydäntulehduksen sekä neurologisia oireita. Borrelioosi on monessa suhteessa kiistanalainen tauti, sillä esimerkiksi sen hoidosta ja mahdollisesta kroonistumisesta on kaksi eriävää näkemystä. Taudin aiheuttaja on Borrelia burgdorferi -bakteeri (Bb). Bb pystyy välttelemään isännän immuunipuolustusta useilla eri mekanismeilla. Bakteeri on korkkiruuvinmuotoinen spirokeetta, mutta voi esiintyä myös epätyypillisinä muotoina, joihin kuuluu esimerkiksi pyöreä mu…

Purification and characterization of the mda-7 tumor suppressor protein expressed in insect cells

2008

Constructing endothelial-specific hypoxia-regulated vectors

2013

Soluspesifinen geenien ilmentyminen on molekyylibiologian yksi suurista tutkimusalueista. Geenit aktivoituvat eri solutyypeissä eri tavoin, vaikka soluissa on sama geneettinen perimä. Tutkimuksissa on löydetty useita tekijöitä mm. enhanserit ja enhanseri-RNA molekyylit, jotka vaikuttavat mm. solukohtaiseen geenin ilmentymiseen. Enhanserit ovat alueita genomissa, jotka sitovat proteiineja ja vuorovaikuttavat transkriptiokompleksin kanssa lisäten geenin transkriptiota. Enhanseri-RNA:t ovat enhaserialueilta tuottettuja RNA-molekyylejä. Geenin ilmentymiseen vaikuttaa todella moniulotteinen säätelyjärjestelmä, kun mukaan lasketaan kaikki transkriptioon ja translaatioon vaikuttavat tekijät. Endot…

A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress

2022

Paxillin is a potential participant in the direct intracellular force transmission which is considered as the foundation of cells sensing and responding to extracellular environment. However, the detection of tension across paxillin has not been achieved due to lacking microsized tools. Herein, a paxillin tension sensor (PaxTs) based on Fluorescence Resonance Energy Transfer (FRET) technique was constructed. PaxTs can be expressed and assembled to FA sites spontaneously to visualize the tension across paxillin with FRET efficiency of ~62.4% in living cells. The tension across paxillin was found to decrease upon shear stress, in which the membrane fluidity and contractility of actin acted as…

The Industrial Applicability of PEA Space Charge Measurements, for Performance Optimization of HVDC Power Cables

2019

Cable manufacturing industries are constantly trying to improve the electrical performance of power cables. During the years, it was found that one of the most relevant degradation factors influencing the cable lifetime is the presence of space charge in the insulation layer. To detect the accumulated charge, the pulsed electro-acoustic (PEA) method is the most used technique. Despite the wide use of the PEA cell, several issues are still present. In particular, the PEA output signal is strongly disturbed by the acoustic waves reflections within the PEA cell. This causes the distortion of the output signal and therefore the misinterpretation of the charge profiles. This, in turn, may result…

Correlation between tissue-harvesting method and donor-site with the yield of spheroids from adipose-derived stem cells

2022

Influence of Nitrogen Doping on Device Operation for TiO₂-Based Solid-State Dye-Sensitized Solar Cells: Photo-Physics from Materials to Devices.

2015

Solid-state dye-sensitized solar cells (ssDSSC) constitute a major approach to photovoltaic energy conversion with efficiencies over 8% reported thanks to the rational design of efficient porous metal oxide electrodes, organic chromophores, and hole transporters. Among the various strategies used to push the performance ahead, doping of the nanocrystalline titanium dioxide (TiO2) electrode is regularly proposed to extend the photo-activity of the materials into the visible range. However, although various beneficial effects for device performance have been observed in the literature, they remain strongly dependent on the method used for the production of the metal oxide, and the influence o…

Postępy w fotowoltanice w Polsce i na świecie = Progress in photovoltaics in Poland and the world

2015

W artykule przedstawiono postęp w zakresie uzyskiwanych sprawności przez ogniwa i moduły fotowoltaiczne (PV) wykonane z różnych materiałów i za pomocą różnych technologii. Zawarto w nim szczegółowe zestawienia tabelaryczne sprawności oraz ich parametrów technicznych. Zwrócono uwagę na nowe obiecujące technologie, które jeszcze nie mają rekordowych sprawności, ale z uwagi na proces ich wytwarzania rokują nadzieje na uzyskanie bardzo dużych sprawności albo tanich wielkopowierzchniowych modułów do komercyjnego zastosowania. Ponadto, omówiono ekonomiczne skutki wdrożenia nowo zawartych zapisów w uchwalonej w lutym 2015 r. Ustawie o Odnawialnych Źródłach Energii (OZE) dla rozwoju rozproszonych i…

Prognostic value of HSP27 in head and neck squamous cell carcinoma: a retrospective analysis of 57 tumours.

2006

Background: The aims of the present study were to assess the prevalence of HSP27 expression in oral squamous cell carcinoma (OSCC) and to verify whether HSP27 can be considered to be a marker of prognosis in patients with OSCC. Materials and Methods: The immunohistochemical expression of HSP27 was evaluated in 57 OSCC, who received standard treatment and monitoring. After grouping for HSP27 expression, OSCCs were statistically analysed for the variables age, gender, histological grading, TNM, staging and survival rate. Univariate and multivariate analyses were performed. Results: HSP27 was found to be reduced in 31 OSCC and was normally expressed in 26 OSCC. The pattern and intensity of HSP…

Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer

2022

Simple Summary Non-small cell lung cancer (NSCLC) is the second most commonly diagnosed cancer and the leading cause of cancer-related death worldwide. Clinical decision-making depends on the histological classification; however, tissue biopsy is frequently not technically feasible due to tumor location or limited tissue samples. Therefore, we propose to find clinical, molecular and histological biomarkers using a minimally invasive approach based on the analysis of the cargo of the blood extracellular vesicles. Exosomes are membranous vesicles present in several biological fluids, which carry biological information to distant tissues, regulating several tumor processes. This study aims to …