Search results for " Complementary"

showing 10 items of 280 documents

Early evolution of metazoan serine/threonine and tyrosine kinases: identification of selected kinases in marine sponges.

1997

The phylum Porifera (sponges) was the first to diverge from the common ancestor of the Metazoa. In this study, six cDNAs coding for protein-serine/threonine kinases (PS/TKs) are presented; they have been isolated from libraries obtained from the demosponges Geodia cydonium and Suberites domuncula and from the calcareous sponge Sycon raphanus. Sequence alignments of the catalytic domains revealed that two major families of PS/TK, the "conventional" (Ca(2+)-dependent) protein kinase C (PKC), the cPKC subfamily, as well as the "novel" (Ca(2+)-independent) PKC (nPKC), form two separate clusters. In each cluster, the sequence from S. raphanus diverges first. To approach the question about the or…

animal structuresSubfamilyDNA ComplementaryMolecular Sequence DataProtein Serine-Threonine KinasesEvolution MolecularSpecies SpecificityGeneticsAnimalsSycon raphanusAmino Acid SequenceCloning MolecularProtein kinase AMolecular BiologyEcology Evolution Behavior and SystematicsProtein kinase CPhylogenyProtein Kinase CCalcareous spongebiologySequence Homology Amino AcidKinaseProtein-Tyrosine Kinasesbiology.organism_classificationPoriferaSuberites domunculaBiochemistryTyrosine kinaseMolecular biology and evolution
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Proton-irradiated breast cells: molecular points of view

2019

Abstract Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and descri…

breast cancer cDNA microarray gene signature proton therapy radiation Breast Breast Neoplasms Cell Line Tumor DNA Complementary Dose-Response Relationship Radiation Female Gene Expression Profiling Gene Expression Regulation Neoplastic Humans Inflammation MCF-7 Cells Oligonucleotide Array Sequence Analysis Phenotype Proton Therapy Radiation Tolerance Radiotherapy ProtonsDNA ComplementaryHealth Toxicology and Mutagenesismedicine.medical_treatmentBreast NeoplasmsCell fate determinationRadiation Tolerancegene signature03 medical and health sciences0302 clinical medicineBreast cancerbreast cancerCell Line TumorRegular Papermedicineproton therapyHumansRadiology Nuclear Medicine and imagingBreastClonogenic assayBiologyProton therapyOligonucleotide Array Sequence Analysis030304 developmental biologyInflammationcDNA microarray0303 health sciencesRadiotherapyChemistryGene Expression ProfilingBreast cancer; radiation; cDNA microarray; gene signature; proton therapyCancerDose-Response Relationship RadiationGene signaturemedicine.diseaseGene Expression Regulation NeoplasticGene expression profilingRadiation therapyradiationPhenotype030220 oncology & carcinogenesisMCF-7 CellsCancer researchFemaleProtons
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Glutamine synthetase from roots of Brassica napus. Nucleotide sequence of a cytosolic isoform.

1994

chemistry.chemical_classificationGene isoformDNA ComplementarybiologyBase SequencePhysiologyMolecular Sequence DataBrassicaNucleic acid sequencePlant ScienceBrassicabiology.organism_classificationGenes PlantIsoenzymesCytosolEnzymeCytosolchemistryBiochemistryGlutamate-Ammonia LigaseComplementary DNAGlutamine synthetaseGeneticsCarbon-Nitrogen LigasesResearch ArticlePlant physiology
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Los beneficios del mar: negocios e inversiones económicas de la oficialidad naval en el siglo XVIII

2019

Throughout the eighteenth century, the Spanish navy was extensively reformed and enhanced as a consequence of the new model of Monarchy that the Bourbons introduced. Among the measures adopted, it is remarkable the erection of the naval officers corps according to the needs and obligations that maritime war had in this century. This group becomes the paradigm of the new professionalization and new training approach, and its members had great relevance due to its great mobility around the empire and the power of the naval institution. This means that a part of the naval officers were placed in privileged positions and at the intersection of diverse political, social and economic networks. Th…

economic benefitsredes económicasnaval officersUNESCO::HISTORIAespecially their participation in the Cadiz trade. It can be concluded that the naval officers of the eighteenth century constituted a quite active economic group that looked for complementary forms of benefit beyond their salary as servants of the King and became essential nodes of the Atlantic economic framework of Spain. beneficios económicosand its members had great relevance due to its great mobility around the empire and the power of the naval institution. This means that a part of the naval officers were placed in privileged positions and at the intersection of diverse politicalinversioneseconomic networksPablo Throughout the eighteenth century18th century 117 138investmentsRevista de historia moderna 529735 2019 45 7107714 Los beneficios del mar: negocios e inversiones económicas de la oficialidad naval en el siglo XVIII Ortega del Cerro [0210-9093 553 Estudis]the Spanish navy was extensively reformed and enhanced as a consequence of the new model of Monarchy that the Bourbons introduced. Among the measures adopted0210-9093 553 Estudis: Revista de historia moderna 529735 2019 45 7107714 Los beneficios del mar: negocios e inversiones económicas de la oficialidad naval en el siglo XVIII Ortega del Cerrosocial and economic networks. This work aims to research this fact through the business and economic investments that these individuals undertook throughout the eighteenth centuryoficiales navales:HISTORIA [UNESCO]it is remarkable the erection of the naval officers corps according to the needs and obligations that maritime war had in this century. This group becomes the paradigm of the new professionalization and new training approachsiglo XVIII
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Type II keratin cDNAs from the rainbow trout: implications for keratin evolution.

2002

From a teleost fish, the rainbow trout Oncorhynchus mykiss, we have cloned and sequenced cDNAs encoding five different type II keratins. The corresponding protein spots, as separated by 2D-PAGE of trout cytoskeletal preparations, have been identified by peptide mass mapping using MALDI mass spectrometry. Three of the sequenced keratins are expressed in the epidermis (subtype IIe), and two in simple epithelia and mesenchymal cells (subtype IIs). The IIs keratins are both orthologs of human K8. This leaves unsequenced only the trace component S3 of the biochemically established trout keratin catalog. A phylogenetic tree has been constructed from a multiple alignment of the rod domains of the …

endocrine systemCancer Researchanimal structuresDNA ComplementaryMolecular Sequence Datamacromolecular substancesPeptide MappingType II keratinEvolution MolecularMesodermSpecies SpecificityAntibody SpecificityKeratinAnimalsHumansProtein IsoformsAmino Acid SequenceCloning MolecularMolecular BiologyZebrafishPhylogenyZebrafishchemistry.chemical_classificationGeneticsMammalsMultiple sequence alignmentintegumentary systembiologyPhylogenetic treeSequence Homology Amino AcidLampreyAntibodies MonoclonalLampreysEpithelial CellsCell Biologybiology.organism_classificationProtein Structure TertiaryTroutchemistryOrgan SpecificityOncorhynchus mykissSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationSharksKeratinsRainbow troutEpidermisSequence AlignmentDevelopmental BiologyDifferentiation; research in biological diversity
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Possible Editing of Alu Transcripts in Blood Cells of Sporadic Creutzfeldt–Jakob Disease (sCJD)

2011

Editing of RNA molecules gained major interest when coding mRNA was analyzed. A small, noncoding, Alu DNA element transcript that may act as regulatory RNA in cells was examined in this study. Alu DNA element transcription was determined in buffy coat from healthy humans and human sporadic Creutzfeldt-Jakob disease (sCJD) cases. In addition, non-sCJD controls, mostly dementia cases and Alzheimer's disease (AD) cases, were included. The Alu cDNA sequences were aligned to genomic Alu DNA elements by database search. A comparison of best aligned Alu DNA sequences with our RNA/cDNA clones revealed editing by deamination by ADAR (adenosine deaminase acting on RNA) and APOBEC (apolipoprotein B ed…

endocrine systemDNA ComplementaryPan troglodytesTranscription GeneticPrionsHealth Toxicology and MutagenesisMolecular Sequence DataAlu elementBiologyToxicologyCreutzfeldt-Jakob SyndromeDNA sequencing03 medical and health scienceschemistry.chemical_compound0302 clinical medicineAlu ElementsTranscription (biology)hemic and lymphatic diseasesComplementary DNAAnimalsHumansCloning Molecular030304 developmental biologyGenetics0303 health sciencesBase SequenceReverse Transcriptase Polymerase Chain ReactionRNAMacaca mulattaMolecular biology3. Good healthgenomic DNAchemistryADARRNARNA EditingSequence Alignment030217 neurology & neurosurgeryDNAJournal of Toxicology and Environmental Health, Part A
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Identification of a Functional Homolog of the Yeast Copper Homeostasis Gene ATX1 from Arabidopsis1

1998

Abstract A cDNA clone encoding a homolog of the yeast (Saccharomyces cerevisiae) gene Anti-oxidant 1(ATX1) has been identified from Arabidopsis. This gene, referred to as CopperCHaperone(CCH), encodes a protein that is 36% identical to the amino acid sequence of ATX1 and has a 48-amino acid extension at the C-terminal end, which is absent from ATX1 homologs identified in animals. ATX1-deficient yeast (atx1) displayed a loss of high-affinity iron uptake. Expression of CCH in the atx1 strain restored high-affinity iron uptake, demonstrating thatCCH is a functional homolog of ATX1. When overexpressed in yeast lacking the superoxide dismutase geneSOD1, both ATX1 and CCHprotected the cell from t…

endocrine systemDNA ComplementarySaccharomyces cerevisiae ProteinsPhysiologyMolecular Sequence DataSaccharomyces cerevisiaeSOD1ArabidopsisGene ExpressionSaccharomyces cerevisiaePlant ScienceFungal ProteinsGene productSuperoxide dismutaseOzoneCopper Transport ProteinsComplementary DNAArabidopsisGene expressionGeneticsHomeostasisAmino Acid SequenceCation Transport ProteinsBase SequenceSequence Homology Amino AcidbiologyArabidopsis ProteinsGenetic Complementation Testbiology.organism_classificationYeastOxidative StressBiochemistrybiology.proteinCarrier ProteinsCopperResearch ArticlePlant Physiology
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Origin of Insulin Receptor-Like Tyrosine Kinases in Marine Sponges

1999

One autapomorphic character restricted to all Metazoa including Porifera [sponges] is the existence of transmembrane receptor tyrosine kinases (RTKs). In this study we screened for molecules from one subfamily within the superfamily of the insulin receptors. The subfamily includes the insulin receptors (InsR), the insulin-like growth factor I receptors, and the InsR-related receptors--all found in vertebrates--as well as the InsR-homolog from Drosophila melanogaster. cDNAs encoding putative InsRs were isolated from the hexactinellid sponge Aphrocallistes vastus, the demosponge Suberites domuncula, and the calcareous sponge Sycon raphanus. Phylogenetic analyses of the catalytic domains of th…

endocrine systemDNA ComplementarySubfamilyMolecular Sequence DataReceptor tyrosine kinaseEvolution MolecularMiceDemospongeCatalytic DomainBotanyAnimalsHumansAmino Acid SequenceSycon raphanusCloning MolecularPhylogenyCephalochordateBase SequenceSequence Homology Amino Acidbiologygeodia cydonium; adhesion receptors; evolutionnutritional and metabolic diseasesSequence Analysis DNAbiology.organism_classificationReceptor InsulinPoriferaRatsSuberites domunculaInsulin receptorSpongeBiochemistrybiology.proteinGeneral Agricultural and Biological Scienceshormones hormone substitutes and hormone antagonistsThe Biological Bulletin
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F-type lectin from the sea bass (Dicentrarchus labrax): purification, cDNA cloning, tissue expression and localization, and opsonic activity.

2009

Recently described biochemical and structural aspects of fucose-binding lectins from the European eel (Anguilla anguilla) and striped bass (Morone saxatilis) led to the identification of a novel lectin family ("F-type" lectins) characterized by a unique sequence motif and a characteristic structural fold. The F-type fold is shared not only with other members of this lectin family, but also with apparently unrelated proteins ranging from prokaryotes to vertebrates. Here we describe the purification, biochemical and molecular properties, and the opsonic activity of an F-type lectin (DlFBL) isolated from sea bass (Dicentrarchus labrax) serum. DlFBL exhibits two tandemly arranged carbohydrate-r…

food.ingredientDNA ComplementaryImmunoblottingAquatic ScienceChromatography AffinityBass (fish)F-type lectin; Dicentrarchus labrax;teleost;emaggluthinins opsoninfoodPhagocytosisOpsonin ProteinsComplementary DNALectinsEnvironmental ChemistryAnimalsDicentrarchus labraxRNA MessengerSea bassCloning MolecularOpsoninemaggluthinins opsoninPhylogenyteleostbiologyBase SequenceLectinGeneral MedicineOpsonin Proteinsbiology.organism_classificationMolecular biologyGene Expression RegulationImmunologybiology.proteinMacrophages PeritonealF lectin sea bass inflammationDicentrarchusBassElectrophoresis Polyacrylamide GelSequence motifF-type lectinFishshellfish immunology
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Clinical pharmacology of citrus aurantium and citrus sinensis for the treatment of anxiety

2018

Objective. The aim of this review is to analyze preclinical and clinical studies investigating the anxiety effects ofCitrus aurantiumorCitrus sinensisessential oils (EOs).Design. The bibliographic research was made on the major scientific databases. Analysis included only articles written in English and published on peer-reviewed scientific journals describing preclinical experiments and clinical trials carried out to investigate the antianxiety effects ofCitrus aurantium or Citrus sinensisEOs on anxiety disorders. Clinical studies reporting the antianxiety effects of products containingCitrus aurantiumorCitrus sinensisEOs in combination with other active substances, including medicinal pla…

medicine.drug_classAnxietyAnxiolyticlaw.invention03 medical and health sciences0302 clinical medicineSettore MED/43 - Medicina LegalelawmedicineCitrus aurantium; Citrus sinensis; Anxiety; Complementary and Alternative Medicine030212 general & internal medicineMedicinal plantsCitrus aurantiumClinical pharmacologyTraditional medicinebusiness.industryPharmacology Citrus aurantium Citrus sinensis Anxietyfood and beverageslcsh:Other systems of medicinelcsh:RZ201-999Clinical trialComplementary and alternative medicineAnxiogenicAnxietymedicine.symptombusiness030217 neurology & neurosurgeryCitrus × sinensisCitrus sinensisAromatherapy
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