Search results for " Controls"

showing 10 items of 30 documents

Quality controls for cell cultures: identification of interspecies cross-contamination by PCR-RFLP analysis of the cytochrome b gene

2012

Cross-contaminations of a cell line with cells of different species represent a potential risk in laboratories handling human and animal cells. Therefore, it is necessary to control such contaminations. Tests based on mitochondrial DNA (mtDNA) are used in forensic analysis, phylogenetic studies and in food authentication. However, the use of mtDNA in quality controls of cell cultures is recent. Mitochondrial sequence differences of closely related animal species are five- to tenfold higher than those of nuclear genes. On the contrary, intraspecies variation in mitochondrial sequences is low in most animal species. Moreover, each cell contains 100–10.000 mitochondrial genomes. The amount of …

QUALITY CONTROLS CELL CULTURES INTERSPECIES CROSS-CONTAMINATION PCR-RFLP ANALYSIS CYTOCHROME B GENE
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Metilazione del DNA in artrite reumatoide

2005

Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…

Settore BIO/18 - Geneticainstead chromosomes of controls were almost uniformly decorated by brilliant grains. Studies on methylation of PTHrP gene promoter 2 performed on five CpG island internal sites using the Methylation-Sensitive Restriction Endonuclease Multiplex (MSREM)-PCR showed that one of the sites nearest the trascription starting point is heavy methylated in a significantly high number of RA patients. Thus RA seems to be characterized by genomewide hypomethylation associated with local hypermethylation like the most part of tumors. This result raises the possibility that susceptibility to lymphomas is related to abnormal DNA methylation levels and suggests the opportunity to evaluate the DNA methylation status in RA patientin fact the demethylating therapies together with diet and life style can act towards an increase of tumor risk. Future studies using a larger number of subjects could confirm these findings.Rheumatoid Arthritis (RA) is a chronic multisystem inflammatory disease characterized by high recurrence of lymphomas as well as hypercalcemia due to PTHrP overexpression. Because of DNA methylation plays a critical role in development of neoplasias we determined in RA patients the global DNA methylation status and local methylation pattern of the CpG island of one of the three promoters of PTHrP gene utilizing molecular and cytogenetic techniques. Investigations performed on DNA from peripheral blood of patients and donors amplified by Methylation-Sensitive Arbitrarily Primed (MeS-AP)-PCR indicated that RA is strongly associated with global DNA hypomethylation. Similarly chromosomal DNA methylation pattern analysis by indirect immunofluorescence technique with anti 5-methylcitosine antibody showed all peripheral lymphocyte metaphases from RA patients with chromosomes weakly fluorescent without discrete grain
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Tailorable Representation of Security Control Catalog on Semantic Wiki

2018

Selection of security controls to be implemented is an essential part of the information security management process in an organization. There exist a number of readily available information security management system standards, including control catalogs, that could be tailored by the organizations to meet their security objectives. Still, it has been noted that many organizations tend to lack even the implementation of the fundamental security controls. At the same time, semantic wikis have become popular collaboration and information sharing platforms that have proven their strength as an effective way to distribute domain-specific information within an organization. This paper evaluates…

World Wide WebInformation security managementKnowledge basebusiness.industryProcess (engineering)Computer scienceInformation sharingControl (management)Information securitybusinessSecurity controlsInformation security management system
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Development of a New Clusterization Method for the GEM-TPC Detector

2022

The Facility for Antiproton and Ion Research FAIR, in Darmstadt Germany, will be one of the largest accelerator laboratories worldwide. The Superconducting FRagment Separator (Super-FRS)* is one of its main components. The Super-FRS can produce, separate and deliver high-energy radioactive beams with intensities up to 1e11 ions/s, covering projectiles from protons up to uranium and it can be used as an independent experimental device. The Gas Electron Multiplier-based Time Projection Chambers (GEM-TPC) in twin configuration is a newly developed beam tracking detector capable of providing spatial resolution of less than 1 mm with a tracking efficiency close to 100% at 1 MHz counting rate. Th…

electrondetectorexperimentPhysics::Instrumentation and DetectorselectronicsPhysics::Accelerator PhysicsNuclear Experiment114 Physical sciencesAccelerator PhysicsMC6: Beam Instrumentation Controls Feedback and Operational AspectsECR
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Emerging Economies Faced With The Downside Of Financial Globalization: Hedges And Way Outs

2009

The underlining assumption of this paper is that developing countries are in a fragile state nowadays. Economically, they have been seriously harmed by the 2008 crisis1 but this is not the end of the story: the future still has pitfalls in which these economies might get trapped, due to their enhanced vulnerability to exogenous shocks generated by financial globalization. Ideologically, the recent events have triggered a serious backlash against capitalism, particularly the Anglo-Saxon template. Getting an insight into the causes and implications of global economic crises is therefore critical for policy-makers in emerging economies. History might be a good adviser in this respect. Lessons …

financial globalizationcurrency criseslcsh:HB71-74international reservesspeculative attackslcsh:Economics as a scienceregulationcapital controlsjel:F41money mercantilismfinancial globalization money mercantilism currency crises international reserves speculative attacks capital controls regulationReview of Economic and Business Studies
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Data from: Ecological limits on diversification of the Himalayan core Corvoidea

2012

Within regions, differences in the number of species among clades must be explained by clade age, net diversification rate, or immigration. We examine these alternatives by assessing historical causes of the low diversity of a bird parvorder in the Himalayas (the core Corvoidea, 57 species present) relative to its more species rich sister clade. The core Corvoidea contain ecologically diverse species spanning a large range of body sizes and elevations. Despite this diversity, on the basis of ecological, morphological, and phylogenetic information, we infer that the best explanation for the low number of species within the core Corvoidea is one in which ecology limits diversification. Within…

medicine and health careCorvoideaDiversity-dependenceLife SciencesMedicineEcological controls
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Decreasing muscle performance associated with increasing disease activity in patients with rheumatoid arthritis

2018

Objectives Increasing evidence suggests that inflammation has a detrimental effect on muscle strength. Our objective was to analyse the association between muscle performance and different disease activity levels in patients with rheumatoid arthritis (RA). Method A total of 199 consecutive outpatients were subject to cross-sectional assessment. Measurements of grip strength, endurance of the upper and lower limbs and trunk strength were combined as a muscle performance composite score (MPCS), using a standardised method. The disease activity for 28 joints (DAS28), radiographs of small joints (Larsen score), rheumatoid factor, body mass index (BMI), comorbidities and anti-rheumatic drugs wer…

nivelreumarheumatoid arthritisMaleINACTIVITYArthritislcsh:MedicineMuscle ProteinsComorbidityBiochemistrySeverity of Illness IndexArthritis RheumatoidGrip strength0302 clinical medicineRisk FactorsSTRENGTHCACHEXIAMedicine and Health SciencesCRITERIAPublic and Occupational Health030212 general & internal medicinelcsh:ScienceMusculoskeletal SystemINDEXPain Measurement2. Zero hungerMultidisciplinaryMusclesWOMENta3141Muscle AnalysisMiddle AgedSports Science3. Good healthBioassays and Physiological AnalysisRheumatoid arthritisStrength TrainingFemaleBONE-MINERAL DENSITYAnatomylihaskuntoResearch Articlemedicine.medical_specialtymuscle fitnessStrength trainingHEALTHY CONTROLSImmunologyRheumatoid ArthritisResearch and Analysis MethodsAutoimmune Diseases03 medical and health sciencesRheumatologyInternal medicineHand strengthmedicineRheumatoid factorHumansMuscle StrengthRisk factorSports and Exercise MedicineMuscle SkeletalExercise030203 arthritis & rheumatologybusiness.industryArthritislcsh:RENERGY-EXPENDITUREBeck Depression InventoryBiology and Life SciencesProteinsPhysical Activitymedicine.diseaseJoints (Anatomy)PHYSICAL-ACTIVITYCross-Sectional StudiesSkeletal MusclesPhysical Fitness3121 General medicine internal medicine and other clinical medicinemuscle strengthPhysical Endurancelcsh:QClinical ImmunologyClinical MedicinebusinesslihasvoimaPLoS ONE
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Oxidative stress and antioxidant levels in patients with anorexia nervosa: A systematic review and exploratory meta-analysis.

2015

Objective To systematically review and meta-analyze oxidative stress and antioxidant markers in anorexia nervosa (AN). Methods Electronic PubMed search from database inception until 12/31/2013. Out of 1062 hits, 29 studies comparing oxidative stress/antioxidant markers between patients with AN and healthy controls (HCs) with a total of 1,729 participants (AN-=-895, HCs-=-834) were eligible. Data about oxidative stress and antioxidant markers, independent of their source, were extracted. We calculated random effects standardized mean differences (SMDs) as effect size measures for outcomes reported in ≥5 studies; others were summarized descriptively. Results Compared to HCs, AN patients showe…

oxidative streantioxidantAnorexia NervosaAntioxidantsHealthy VolunteersOxidative StressYoung AdultCross-Sectional StudiesPsychiatry and Mental Healthhealthy controlsHumansFemaleanorexia nervosa; antioxidant; healthy controls; oxidative stress; Anorexia Nervosa; Antioxidants; Biomarkers; Cross-Sectional Studies; Female; Healthy Volunteers; Humans; Oxidative Stress; Young Adult; Psychiatry and Mental HealthBiomarkersThe International journal of eating disorders
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Modeling and Backstepping Control of the Electronic Throttle System

2013

Published version of an article in the journal: Mathematical Problems in Engineering. Also available from the publisher at: http://dx.doi.org/10.1155/2013/871674 Open access Electronic throttle is widely used in modern automotive engines. An electronic throttle system regulates the throttle plate angle by using a DC servo motor to adjust the inlet airflow rate of an internal combustion engine. Its application leads to improvements in vehicle drivability, fuel economy, and emissions. In this paper, by taking into account the dynamical behavior of the electronic throttle, the mechanism model is first built, and then the mechanism model is transformed into the state-space model. Based on the s…

state-space modelingbacksteppingAutomotive engineEngineeringArticle SubjectGeneral Mathematicsback-stepping controlsinternal combustion enginesVDP::Technology: 500::Electrotechnical disciplines: 540ServomotorThrottleControl theorybackstepping design techniquecomputer simulationCruise controlbusiness.industrylcsh:MathematicsGeneral Engineeringbackstepping controllertracking performancelcsh:QA1-939fuel economyVDP::Mathematics and natural science: 400::Mathematics: 410Internal combustion enginelcsh:TA1-2040BacksteppingControl systemdynamical behaviorselectronic throttleautomotive enginebusinesslcsh:Engineering (General). Civil engineering (General)Mathematical Problems in Engineering
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Supporting Cyber Resilience with Semantic Wiki

2016

Cyber resilient organizations, their functions and computing infrastructures, should be tolerant towards rapid and unexpected changes in the environment. Information security is an organization-wide common mission; whose success strongly depends on efficient knowledge sharing. For this purpose, semantic wikis have proved their strength as a flexible collaboration and knowledge sharing platforms. However, there has not been notable academic research on how semantic wikis could be used as information security management platform in organizations for improved cyber resilience. In this paper, we propose to use semantic wiki as an agile information security management platform. More precisely, t…

ta113021110 strategic defence & security studiesEngineeringKnowledge managementCloud computing securitybusiness.industryStandard of Good Practiceinformation security management0211 other engineering and technologies02 engineering and technologyInformation securitycyber resiliencesemantic wikiSecurity information and event managementrisk managementSecurity controlsResilience (organizational)World Wide WebITIL security managementInformation security management020204 information systems0202 electrical engineering electronic engineering information engineeringbusiness
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