Search results for " DAMAGE"

showing 10 items of 1139 documents

The Putative Metal Coordination Motif in the Endonuclease Domain of Human Parvovirus B19 NS1 Is Critical for NS1 Induced S Phase Arrest and DNA Damage

2011

The non-structural proteins (NS) of the parvovirus family are highly conserved multi-functional molecules that have been extensively characterized and shown to be integral to viral replication. Along with NTP-dependent helicase activity, these proteins carry within their sequences domains that allow them to bind DNA and act as nucleases in order to resolve the concatameric intermediates developed during viral replication. The parvovirus B19 NS1 protein contains sequence domains highly similar to those previously implicated in the above-described functions of NS proteins from adeno-associated virus (AAV), minute virus of mice (MVM) and other non-human parvoviruses. Previous studies have show…

apoptotic cell deathDNA repairDNA damagevirusesAmino Acid MotifsDNA Mutational AnalysisApoptosisSpodopteraViral Nonstructural ProteinsVirus ReplicationApplied Microbiology and Biotechnology03 medical and health scienceschemistry.chemical_compound0302 clinical medicineControl of chromosome duplicationparvoviral infectionParvovirus B19 HumanAnimalsHumansMolecular BiologyEcology Evolution Behavior and SystematicsS phase030304 developmental biology0303 health sciencesbiologyParvovirushost cell DNA damagevirus diseasesHep G2 CellsCell BiologyEndonucleasesbiology.organism_classificationMolecular biology3. Good healthchemistryViral replicationS Phase Cell Cycle CheckpointsMutagenesis Site-Directed030211 gastroenterology & hepatologyDNAMinute virus of miceResearch PaperDNA DamageDevelopmental BiologyInternational Journal of Biological Sciences
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The Metabolic Syndrome and Its Relationship to Hypertensive Target Organ Damage

2007

High blood pressure is often associated with various metabolic abnormalities, including abdominal obesity, dyslipidemia, elevated plasma glucose, and insulin resistance, which are the main features of the metabolic syndrome. The metabolic syndrome is extremely common worldwide. This high prevalence is of considerable concern because several studies suggest that the metabolic syndrome carries an increased risk for cardiovascular events. Several lines of evidence seem to indicate that the metabolic syndrome is associated with an increased prevalence of preclinical cardiovascular and renal changes, such as left ventricular hypertrophy, microalbuminuria, impaired aortic elasticity, and early ca…

arterial hypertensionmedicine.medical_specialtySettore MED/09 - Medicina InternaEndocrinology Diabetes and MetabolismLeft ventricular hypertrophyRisk AssessmentInsulin resistanceRisk Factorstarget organ damageInternal medicinePrevalenceInternal MedicinemedicineHumansAbdominal obesityReview Paperbusiness.industrymedicine.diseaseMetabolic syndromeBlood pressureCardiovascular DiseasesHypertensionCardiologyMicroalbuminuriaMetabolic syndromemedicine.symptomCardiology and Cardiovascular MedicineRisk assessmentbusinessDyslipidemiaThe Journal of Clinical Hypertension
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Echocardiographic Evaluation of the Cardiac Chambers in Asthmatic Patients: The BADA (Blood Pressure Levels, Clinical Features and Markers of Subclin…

2022

The “Blood pressure levels, clinical features and markers of subclinical cardiovascular Damage of Asthma patients” (BADA) study is aimed at defining the cardiovascular risk profile and the markers of subclinical and clinical vascular and cardiac damage in asthmatic patients. Very few studies have assessed asthmatic patients without concomitant heart disease through a transthoracic echocardiogram. The goal of the present study is to investigate the prevalence of morphology and/or function changes in the cardiac chambers of a sample of 86 patients with chronic asthma, referred to the dedicated outpatient unit of the Division of Respiratory Diseases of the AOUP “P. Giaccone&r…

asthma; cardiovascular risk; echocardiography; cardiac damage; essential hypertension; inhaled corticosteroid therapy; beta-adrenergic agonist agentscardiovascular riskbeta-adrenergic agonist agentessential hypertensioninhaled corticosteroid therapy.Medicine (miscellaneous)cardiac damageechocardiographyasthma
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Vecchi e nuovi problemi in tema di intervento dei creditori nell’esecuzione (note a margine di Cass. S.U. n. 61 del 7 gennaio 2014)

2015

Nel contributo in oggetto l'autore, muovendo dalla decisione delle Sezioni Unite della Cassazione n. 61 del 7 gennaio 2014, coglie l'occasione per riesaminare le vecchie e nuove problematiche sottese all’intervento dei creditori nel processo esecutivo. Il primo tema affrontato è quello della par condicio creditorum, che viene esaminata nella sua storia evolutiva dal Code Napoleon, al suo periodo di massima estensione, dato dal codice del 1940, fino al suo ridimensionamento a seguito delle riforme del 2005. Esamina quindi la possibilità del possibile superamento quoad effectum della distinzione tra intervento di creditori con titolo e senza titolo, alla luce delle evoluzioni della giurisprud…

b) the same par condicio creditorum principle and the consequent possibility of intervention of third party in the execution. It concludes however that a revolution like the aforesaid explained would be only apparently advantageouOld and new problems related to the intervention of creditors in the expropriation (marginal notes at Cass. Sez. Un. January 7 2014 n. 61) Moving from the decision of the Sezioni Unite n. 61/2014 the author takes the opportunity to re-examine old and new problems related to the intervention of creditors in the forceable execution. The first major theme concerns par condicio creditorum which is examined in its evolutionary history since the Code Napoleon through its period of maximum extension (the Code of 1940) until its reduction as a result of the reforms of 2005. The author examines the possibility to overcome quoad effectum the distinction between sine titulo et cum titulo interveners in light of jurisprudence’s evolution between 1978 2009 and 2014. The possibility in particular concerns those creditors sine titulo the where credit has not been contested (ex art. 499 c.p.c.) by the debtor seek payment and partecipate to distribution. In the last section the author examines the possibilities for other creditors to contest the intervention of the creditor sine titulo ex art. 499 c.p.c. (utendo iuribus in subrogation in case of inaction of the debtor) or in the distribution phase ex art. 512 c.p.c. He concludes that the only person that will be really damaged if he doesn’t contest - ex art. 499 c.p.c. - the credit it is precisely the debtor. He also notes that in the legislation of the last decade the apparent effort to reduce the numbers of judicial enquiry incidental to forceable execution will never be achieved unless it’s eliminated in radice: a) the existence in our system of extrajudicial enforcement titulaSettore IUS/15 - Diritto Processuale Civilesince it would lead to increase autonomous expropriations (one for each creditor who can today intervene) and to increment judicial enquiries in order to form enforceable judgments
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The Effect of Bark Stripping by Deer (Cervus elaphus L.) on Biometric Parameters of the Scots Pine

2022

Bark stripping by red deer (Cervus elaphus L.) is one of the most acute instances of damage wrought by these animals in a forest. Because of its location, bark stripping causes damage to the thickest and most valuable part of the tree trunk, which lowers the quality of the wood due to technical flaws. The research was conducted in 25 sampling sites of pine stands aged 35 years in a mixed, fresh forest habitat in south-western Poland. The study was aimed at assessing the influence of bark damage by deer on biometric parameters of pine stands (Pinus sylvestris L.). The assessment involved the diameter at breast height, height, basal area and volume of the tree stands. The results revealed a s…

bark stripping; damage; deer; DBH; basal area; stand volume; Pinus sylwestris L.Applied Sciences-Basel
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Drivers of topoisomerase II poisoning mimic and complement cytotoxicity in AML cells

2019

Recently approved cancer drugs remain out-of-reach to most patients due to prohibitive costs and only few produce clinically meaningful benefits. An untapped alternative is to enhance the efficacy and safety of existing cancer drugs. We hypothesized that the response to topoisomerase II poisons, a very successful group of cancer drugs, can be improved by considering treatment-associated transcript levels. To this end, we analyzed transcriptomes from Acute Myeloid Leukemia (AML) cell lines treated with the topoisomerase II poison etoposide. Using complementary criteria of co-regulation within networks and of essentiality for cell survival, we identified and functionally confirmed 11 druggabl…

biologyCombination therapybusiness.industryTopoisomeraseMyeloid leukemiatopoisomerase II poisonscombination therapyCell killingOncologygene expressioncancer essentialitybiology.proteinmedicineCancer researchDNA damageCytotoxic T cellCytotoxicitybusinessEtoposidePI3K/AKT/mTOR pathwayResearch Papermedicine.drugOncotarget
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Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi

1999

The effects of different polychlorinated biphenyls (PCBs) including toxic coplanar non-ortho (PCB77) and non-planar mono-ortho (PCB118) and di-ortho (PCB153) congeners on the extent of DNA damage as well as on the expression of heat shock protein 70 (HSP70) were investigated in the marine sponge Suberites domuncula Olivi (Porifera; Demospongiae). A time-dependent increase in the number of DNA single-strand breaks, expressed as strand scission factor (SSF), was found after injection of a single dose of 25 mu g of PCB 118 or PCB 153 per gram wet mass of S. domuncula, using Fast Micromethod assay, which is based on the unwinding of DNA under alkaline conditions. The number of strand breaks ind…

biologyDNA damageAquatic Sciencebiology.organism_classificationMolecular biologyMicrobiologyHsp70Suberites domunculaSpongechemistry.chemical_compoundDNA damage; heat shock protein hsp70; polychlorinated biphenyls; environmental stresschemistryHeat shock proteinGene expressionEcology Evolution Behavior and SystematicsDNASuberites
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[33] Use of repair endonucleases to assess DNA damage by peroxynitrite

1999

Publisher Summary This chapter discusses the use of repair endonucleases to assess DNA damage by peroxynitrite. Repair endonucleases allow a convenient quantification of various types of oxidative modifications induced by peroxynitrite, both in cultured cells and in cell-free DNA. The high sensitivity of the assays allows highly ectotoxic exposure conditions to be avoided—as well as the generation of secondary DNA modifications—that often become a problem at high levels of damage because primary DNA oxidation products can be orders of magnitude more reactive than the original bases, as demonstrated for the reaction of 8-hydroxyguanine with singlet oxygen. The ratio of the various types of m…

biologyDNA damageDNA repairSinglet oxygenDNA oxidationmedicine.disease_causechemistry.chemical_compoundchemistryBiochemistrybiology.proteinmedicineEndodeoxyribonucleasesPeroxynitriteOxidative stressDNA
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Photoinduced DNA Lesions in Dormant Bacteria. The Peculiar Route Leading to Spore Photoproduct Unraveled by Multiscale Molecular Dynamics

2020

Some bacterial species enter a dormant state in the form of spores to resist to unfavorable external conditions. Spores are resistant to a wide series of stress agents, including UV radiation, and can last for tens to hundreds of years. Due to the suspension of biological functions such as DNA repair, they accumulate DNA damage upon exposure to UV radiation. Differently from active organisms, the most common DNA photoproduct in spores are not cyclobutane pyrimidine dimers, but rather the so-called spore photoproduct. This non-canonical photochemistry results from the dry state of DNA and the binding to small acid soluble proteins that drastically modify the structure and photoreactivity of …

biologyDNA repairChemistryDNA damagefungiPyrimidine dimerbiology.organism_classificationSporechemistry.chemical_compoundMolecular dynamicsNucleic acidBiophysicsBacteriaDNA
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Squaraine Dyes for Photodynamic Therapy: Mechanism of Cytotoxicity and DNA Damage Induced by Halogenated Squaraine Dyes Plus Light (>600 nm)¶

2004

Halogenated squaraine dyes 1 and 2 possess favorable photophysical and in vitro photobiological properties that make these new class of molecules interesting for photodynamic therapeutic applications. For a better understanding of the mechanism of their photobiological activity, we have analyzed the DNA damage and the cytotoxicity induced by these photosensitizers in mammalian cells and cell-free systems in the presence and absence of various additives and scavengers. Both photoactivated squaraines were found to be similar efficient in inducing single-strand breaks (SSB) in cell-free DNA when compared with the cellular DNA. Superoxide dismutase and catalase did not show any influence. Howev…

biologySinglet oxygenDNA damagemedicine.medical_treatmentPhotodynamic therapyGeneral MedicineGlutathionePhotochemistryBiochemistryIn vitroSuperoxide dismutasechemistry.chemical_compoundchemistrybiology.proteinmedicinePhysical and Theoretical ChemistryCytotoxicityDNAPhotochemistry and Photobiology
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