Search results for " DNA"

showing 10 items of 2475 documents

Rapid and eco-friendly synthesis of graphene oxide-silica nanohybrids

2014

The increasing interest in Graphene oxide (GO) is due to many issues: the presence of both sp2-conjugated atoms and oxygen-containing functional groups provides a strong hydrophilicity and the possibility to further functionalize it with other molecules (i.e. π-π interactions covalent attachment etc.) [1]. Furthermore since the GO is biocompatible and noncytotoxic many studies have been recently focused on the development of GO-based nanodevices for bioimaging DNA detection drug delivery. Due to their low cytotoxicity and large internal surface area silica nanoparticles have been taken into account as promising material for biolabeling and drug loading/delivery. Particular consideration has recently been demonstrated for GO-silica composites because of the potentialities for electrical applications their chemical inertia and stability toward ions exposure. The possibility to combine the extraordinary properties of GO and silica offers several advantages for the realization of nanoprobes for biological applications and of biosensor [12]. The strategy for the fabrication of GO-nanosilica nanohybrids can be schematized as follows: (i) synthesis of GO by oxidizing graphite powder with the method described by Marcano et al. [3] (ii) Preparation of oxygen-loaded silica nanoparticles by thermal treatments in controlled atmosphere in order to induce high NIR emission at 1272 nm from high purity silica nanoparticles. (iii) preparation of GrO-silica nanohybrid films via rapid solvent casting in water. The nanohybrids were tested by XPS FTIR Raman analysis UV photoluminescence analysis TGA Zeta potential measurements electrical tests AFM and SEM. Several nanohybrids were prepared by combining two different typologies of GO and two different samples of silica.
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Quaternary diversification in European alpine plants: pattern and process

2004

Molecular clock approaches applied previously to European alpine plants suggest that Primula sect. Auricula , Gentiana sect. Ciminalis and Soldanella diversified at the beginning of the Quaternary or well within this period, whereas Globularia had already started diversifying in the (Late–)Tertiary. In the first part of this paper we present evidence that, in contrast to Globularia and Soldanella , the branching patterns of the molecular internal transcribed spacer phylogenies of both Primula and Gentiana are incompatible with a constant–rates birth–death model. In both of these last two taxa, speciation probably decreased through Quaternary times, perhaps because of some niche–filling pro…

Time FactorsExtinctionGeographyModels GeneticbiologyRange (biology)EcologyGenetic VariationSequence Analysis DNAPlantsbiology.organism_classificationGeneral Biochemistry Genetics and Molecular BiologySoldanellaEuropeEvolution MolecularGlobulariaPrimulaSpecies SpecificityRegression AnalysisGlacial periodGeneral Agricultural and Biological SciencesQuaternaryMolecular clockResearch ArticlePhilosophical Transactions of the Royal Society of London. Series B: Biological Sciences
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Tracing European Founder Lineages in the Near Eastern mtDNA Pool

2000

Founder analysis is a method for analysis of nonrecombining DNA sequence data, with the aim of identification and dating of migrations into new territory. The method picks out founder sequence types in potential source populations and dates lineage clusters deriving from them in the settlement zone of interest. Here, using mtDNA, we apply the approach to the colonization of Europe, to estimate the proportion of modern lineages whose ancestors arrived during each major phase of settlement. To estimate the Palaeolithic and Neolithic contributions to European mtDNA diversity more accurately than was previously achievable, we have now extended the Near Eastern, European, and northern-Caucasus d…

Time FactorsHaplogroup HLineage (evolution)Extrachromosomal InheritanceBiologyDNA MitochondrialHaplogroupMiddle East03 medical and health sciencesGene FrequencyDemic diffusionGeneticsHumansGenetics(clinical)PhylogenyGenetics (clinical)030304 developmental biologyGenetics0303 health sciences030305 genetics & heredityGenetic VariationGene PoolArticlesHaplogroup L3Emigration and ImmigrationFounder EffectEuropeDatabases as TopicHaplotypesMutagenesisEvolutionary biologyGenealogical DNA testHuman mitochondrial DNA haplogroupFounder effectThe American Journal of Human Genetics
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Is tubulin the sole antigen recognized by a putative anti-bursicon antibody?

1999

Abstract A 56-kDa polypeptide suspected to be the tanning hormone `bursicon' was analyzed using the monoclonal antibody (mAb) 01C10 of Song and Ma. We studied the beetle Tenebrio molitor, for which data on bursicon have been recently published. After purification by two-dimensional gel electrophoresis of brain proteins, the immunoreactive 56-kDa polypeptide was trypsinated and microsequenced. The obtained sequences revealed a high homology with α- and β-tubulins. In a complementary study, immunoreactive clones were isolated, using the 01C10 mAb, from a library in expression vector obtained from Drosophila melanogaster head cDNAs. Again, the isolated clones were found, after cDNA sequencing,…

Time FactorsInvertebrate HormonesPhysiologymedicine.drug_classBlotting WesternAntibody AffinityEnzyme-Linked Immunosorbent AssayMonoclonal antibodyBiochemistryAntigenTubulinImmunoscreeningmedicineAnimalsTenebrioMolecular BiologyCells CulturedChromatography High Pressure LiquidBursiconGene LibraryGel electrophoresisExpression vectorbiologyAntibodies MonoclonalBrainSequence Analysis DNAMolecular biologyTubulinbiology.proteinChromatography GelDrosophilaElectrophoresis Polyacrylamide GelAntibodyComparative biochemistry and physiology. Part B, Biochemistrymolecular biology
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Inhibitory influence of chromogranin A N-terminal fragment (vasostatin-1) on the spontaneous contractions of rat proximal colon

2005

Very little is known about the role played by CGA and its fragments in the gastrointestinal physiology. We have studied the role of CGA N-terminal fragments in the regulation of intestinal smooth muscle contractility by measuring the influence of recombinant CGA 1-78 (VS-1) and synthetic CGA 7-57 peptides on the spontaneous mechanical activity of rat proximal colon in vitro. The mechanical activity was recorded as changes in the intraluminal pressure. VS-1 (0.1-30 nM) and CGA 7-57 (10-300 nM) produced concentration-dependent inhibitory effects, characterized by a progressive decrease in the mean amplitude of circular muscle spontaneous contractions, without affecting the resting tone. The r…

Time FactorsPhysiologyClinical BiochemistrySettore BIO/09 - FisiologiaBiochemistrylaw.inventionchemistry.chemical_compoundEndocrinologylawEnzyme InhibitorsIntestinal smooth muscleOxadiazolesCGA-derived peptideVasostatin-1Chromogranin ASmooth muscle contractionRecombinant ProteinsNG-Nitroarginine Methyl EsterRecombinant DNATetrodotoxinMuscle Contractionendocrine systemmedicine.medical_specialtyColonTetrodotoxinBiologyInhibitory postsynaptic potentialApaminNitric oxideCellular and Molecular NeuroscienceQuinoxalinesInternal medicineChromograninsPressuremedicineAnimalsRats WistarDose-Response Relationship DrugMuscle SmoothNitric oxidePeptide FragmentsIn vitroProtein Structure TertiaryRatsGastrointestinal TractEndocrinologyApaminchemistrybiology.proteinChromogranin ACalreticulinPeptidesRegulatory Peptides
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Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector

2008

An ideal gene therapy vector should enable persistent transgene expression without limitations of safety and reproducibility. Here we report the development of a non-viral episomal plasmid DNA (pDNA) vector that appears to fulfil these criteria. This pDNA vector combines a scaffold/matrix attachment region (S/MAR) with a human liver-specific promoter (alpha1-antitrypsin (AAT)) in such a way that long-term expression is enabled in murine liver following hydrodynamic injection. Long-term expression is demonstrated by monitoring the longitudinal luciferase expression profile for up to 6 months by means of in situ bioluminescent imaging. All relevant control pDNA constructs expressing luciferas…

Time FactorsTransgeneGenetic VectorsGene ExpressionMice Inbred StrainsGene deliveryBiologyTransfectionViral vectorInjectionsMiceSettore MED/38 - Pediatria Generale E SpecialisticaGene expressionGeneticsGene silencingAnimalsHepatectomyHumansLuciferaseTransgenesScaffold/matrix attachment regionLuciferasesPromoter Regions GeneticMolecular BiologyGenetic Therapynon-viral episomal plasmid DNA (pDNA) vector S/MAR element hydrodynamic injection.DNA MethylationMatrix Attachment RegionsMolecular biologyImmunohistochemistryLiveralpha 1-AntitrypsinDNA methylationMolecular MedicinePlasmids
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Intraspecific diversity of Lactobacillus curvatus, Lactobacillus plantarum, Lactobacillus sakei, and Leuconostoc mesenteroides associated with vacuum…

2006

The intraspecific diversity of Leuconostoc mesenteroides, Lactobacillus curvatus, Lactobacillus sakei, and Lactobacillus plantarum was analyzed by randomly amplified polymorphic DNA (RAPD) PCR with universal primers M13 and T3. The study included 100 reference strains and 210 isolates recovered from two vacuum-packed Spanish meat products, fiambre de magro adobado and morcilla, previously identified by rDNA-restriction fragment length polymorphism profiles. The RAPD-M13 profiles identified isolates at species level in L. plantarum and L. mesenteroides, while RAPD-T3 provided profiles in L. sakei. The combination of RAPD-M13 and RAPD-T3 fingerprints revealed a total of 17 profiles in L. mese…

Time FactorsVacuumFood spoilageFood ContaminationMicrobiologyMicrobiologylaw.inventionSpecies SpecificitylawFood Preservationparasitic diseasesAnimalsHumansPhylogenyPolymerase chain reactionbiologyFood Packagingfood and beveragesLactobacillaceaebiology.organism_classificationRandom Amplified Polymorphic DNA TechniqueLactobacillus sakeiRAPDMeat ProductsLactobacillusConsumer Product SafetyLeuconostoc mesenteroidesLeuconostocLactobacillus plantarumBacteriaLactobacillus plantarumFood Science
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Early kinetics of Torque Teno virus DNA load and BK polyomavirus viremia after kidney transplantation

2019

Torque teno virusPolyomavirus InfectionsTransplantationTorque teno virusbusiness.industryViremiaViral Loadmedicine.diseasemedicine.disease_causeKidney TransplantationVirologyBK virusTorque teno virus DNAKineticsTumor Virus InfectionsInfectious DiseasesBK VirusDNA ViralHumansMedicineKidney DiseasesViremiabusinessKidney transplantationTransplant Infectious Disease
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Dichrorampha dinarica, new species, a century of confusion in European lepidopterology (Lepidoptera: Tortricidae) resolved by combining morphology an…

2012

Dichrorampha dinarica, new species, is described from the Dinaric mountains (Macedonia, Bosnia and Herzegovina). The newspecies was formerly confused with D. ligulana (Herrich-Schäffer, 1851) from the Alps and D. rilana Drenowsky, 1909 fromthe Rila mountains (Bulgaria). Therefore, a re-description of D. rilana is provided based on topotypical specimens. The diag-nostic morphological characters of the involved species, supported by significant divergence of the mtDNA barcode (COI fragment of 658 bp), are discussed in detail.

TortricidaeMitochondrial DNAmedicine.medical_specialtyInsectaArthropodabiologyZoologyBiodiversityDichrorampha dinaricabiology.organism_classificationDNA barcodingLepidopteraLepidoptera genitaliaBotanyTortricidaemedicineAnimaliaAnimal Science and ZoologyTaxonomy (biology)EndemismLepidopterologyEcology Evolution Behavior and SystematicsTaxonomyZootaxa
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Cloning and expression of a type IX-like collagen in tissues of the ascidian Ciona intestinalis

2002

Collagens are highly preserved proteins in invertebrates and vertebrates. To identify the collagens in urochordates, the total RNA extracted from the pharynx of the ascidian Ciona intestinalis was hybridized with a heterologous probe specific for the echinoderm Paracentrotus lividus fibrillar type I-like larval collagen. Using this probe, two main bands (i.e. 6 and 2.8 kb mRNA) were observed on Northern blot hybridization. The cDNA library prepared from poly(A)+RNA extracted from pharyngeal tissue was screened and a cDNA that specifies a type IX-like collagen was identified. This molecule presents a conceptual open reading frame for a protein containing 734 amino acids. In particular, we sh…

Transcription GeneticAscidianMolecular Sequence DataBiophysicsIn situ hybridizationcDNA libraryBiochemistryCollagen Type IXMiceStructural BiologyComplementary DNAGeneticsAnimalsHumansCiona intestinalisTissue DistributionNorthern blotAmino Acid SequenceRNA MessengerCloning MolecularType IX-like collagenPeptide sequencePhylogenyGene LibraryMessenger RNAbiologyBase SequenceSequence Homology Amino AcidcDNA libraryRNAbiology.organism_classificationMolecular biologyCiona intestinalismRNA localizationSequence Alignment
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