Search results for " DOM"
showing 10 items of 2750 documents
Asthmatic changes in mice lacking T-bet are mediated by IL-13
2005
Mice with a targeted deletion of the T-bet gene exhibit spontaneous airway hyperresponsiveness (AHR), airway inflammation, enhanced recovery of T(h)2 cytokines from bronchoalveolar lavage fluid, sub-epithelial collagen deposition and myofibroblast transformation. Here we analyze the mechanisms responsible for the chronic airway remodeling observed in these mice. CD4+ T cells isolated from the lung of T-bet-deficient mice were spontaneously activated CD44(high)CD69(high) memory T cells, with a typical T(h)2 cytokine profile. Neutralization of IL-13 but not IL-4 resulted in amelioration of AHR in airways of mice lacking T-bet. IL-13 blockade also led to reduced eosinophilia and decreased vime…
The receptor NLRP3 is a transcriptional regulator of TH2 differentiation.
2015
The receptor NLRP3 is involved in the formation of the NLRP3 inflammasome that activates caspase-1 and mediates the release of interleukin 1β (IL-1β) and IL-18. Whether NLRP3 can shape immunological function independently of inflammasomes is unclear. We found that NLRP3 expression in CD4(+) T cells specifically supported a T helper type 2 (TH2) transcriptional program in a cell-intrinsic manner. NLRP3, but not the inflammasome adaptor ASC or caspase-1, positively regulated a TH2 program. In TH2 cells, NLRP3 bound the Il4 promoter and transactivated it in conjunction with the transcription factor IRF4. Nlrp3-deficient TH2 cells supported melanoma tumor growth in an IL-4-dependent manner and …
The Transcription Factor T-bet Regulates Mucosal T Cell Activation in Experimental Colitis and Crohn's Disease
2002
The balance between pro and antiinflammatory cytokines secreted by T cells regulates both the initiation and perpetuation of inflammatory bowel diseases (IBD). In particular, the balance between interferon (IFN)-gamma/interleukin (IL)-4 and transforming growth factor (TGF)-beta activity controls chronic intestinal inflammation. However, the molecular pathways that evoke these responses are not well understood. Here, we describe a critical role for the transcription factor T-bet in controlling the mucosal cytokine balance and clinical disease. We studied the expression and function of T-bet in patients with IBD and in mucosal T cells in various T helper (Th)1- and Th2-mediated animal models …
Docosahexaenoic acid modulates the expression of T-bet and GATA-3 transcription factors, independently of PPARα, through suppression of MAP kinase ac…
2009
The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, respectively, T(H)1 and T(H)2 phenotype. The T-cells from PPARalpha(null) mice secreted higher IFN-gamma and lower IL-4 concentrations than WT T-cells. Furthermore, the deletion of PPARalpha gene in T-cells resulted in the upregulation of T-bet and downregulation of GATA-3 both at mRNA and protein levels. DHA exerted not only an inhibitory effect on T-cell proliferation, but also…
IL-28A Is a Key Regulator of T-Cell–Mediated Liver Injury via the T-Box Transcription Factor T-Bet
2006
Background & Aims: T-cell–mediated fulminant hepatitis is a potentially life-threatening event for which the underlying pathogenic mechanisms are not fully understood. Here, we demonstrate a key regulatory role of IL-28A in T-cell–mediated hepatitis. Methods: We cloned the murine IL-28A gene by reverse-transcription polymerase chain reaction, assessed the effects of recombinant IL-28A, and generated IL-28A–transgenic mice. Results: IL-28A induced TH1 cytokine production by CD4+ T lymphocytes in a T-bet–dependent manner and was up-regulated in a murine model of T-cell–mediated hepatitis upon Con A administration. In vivo, CD4+ T cells from newly created IL-28A–transgenic animals revealed an …
ESPERIENZE SFAVOREVOLI INFANTILI E VIOLENZA DOMESTICA IN ETÀ ADULTA
2014
La letteratura internazionale ha identificato relazioni interpersonali conflittuali/disturbate in età adulta come sequele a lungo termine di situazioni traumatiche precoci(Bifulco& Thomas, 2013; Valdez et al., 2013). Una storia di abusi infantili(emotivi, fisici, sessuali…) può aumentare il rischio di incorrere in altre forme di maltrattamento in età adulta, inclusa la violenza domestica (IPV-Intimate Partner Violence) (Widom et al., 2008; Kim et al, 2009; Alexander, 2009).Risulta pertanto importante l’approfondimento delle condizioni di sviluppo sottostanti allo strutturarsi di tali problematiche. La Childhood Experience of Care and Abuse(CECA)Interview (Bifulcoet al., 1994; Giannone, Schi…
Assessing Hydrus-2D model to investigate the effects of different on-farm irrigation strategies on potato crop under subsurface drip irrigation
2019
The objective of this paper was to assess the performance of Hydrus-2D model to simulate the effects of different on-farm irrigation strategies applied on potato crop. The ability of the model to simulate the stress coefficient (Ks), obtained as the ratio between actual and maximum transpiration, and to define the productive function of potato crop under the semi-arid conditions of central Tunisia were also evaluated. Experiments were carried out on potato crop under full (FI) and deficit irrigation (DI) and two different water qualities supplied by means of a subsurface drip irrigation system. Results evidenced that the model, despite some discrepancies locally observed, can fairly accurat…
Apoptosis in marine sponges: a biomarker for environmental stress (cadmium and bacteria)
1998
The marine demosponge Suberites domuncula is abundantly present on muddy sand bottoms, both in the open sea and in harbors. In the present study it is shown that exposure of S. domuncula to cadmium (CdCl2) in concentrations ranging from 0.01 to 5.0 g ml−1 for up to 5 d results in apoptotic fragmentation of DNA. Kinetics experiments revealed that after 24 h a significant increase of DNA fragmentation already occurred. Besides cadmium a second stimulus was identified to also cause apoptosis in this species, namely exposure to heat-treated Escherichia coli. In order to support the finding that both cadmium and E. coli induce apoptosis in the sponge, expression of the apoptotic gene MA-3 was st…
The sponge silicatein-interacting protein silintaphin-2 blocks calcite formation of calcareous sponge spicules at the vaterite stage
2013
Ca-carbonate, the inorganic matrix of the spicules from the calcareous sponges, is formed as the result of an enzyme-catalyzed reaction with the carbonic anhydrase [CA] as a decisive component. The growth and the morphology of the spicules are genetically controlled, and are taxon-specific. In the present study it is shown that the silicatein-interacting protein silintaphin-2 is present at the surface of the siliceous spicules of the demosponge Suberites domuncula and prevents the association of calcareous crystals synthesized in vitro to these skeletal elements. Silintaphin-2 comprises a Ca2+-binding domain that is formed by a 22 amino acid-long peptide, N-DDDSQGEIQSDMAEEEDDDNVD-C. This ve…
The Neapolitan Yellow Tuff caldera offshore the Campi Flegrei: Stratal architecture and kinematic reconstruction during the last 15 ky
2014
In this study we integrate high-resolution swath bathymetry, single channel reflection seismic data and gravity core data, to provide new insights into the shallow structure and latest Quaternary to Holocene evolution of the submerged sector of the Neapolitan Yellow Tuff (NYT) caldera (Campi Flegrei) in the Pozzuoli Bay. The new data allow for a reconstruction of the offshore geometry of the NYT caldera collapse-ring fault system, along with the style and timing of deformation of the inner caldera resurgence.Our interpretation shows that the NYT eruption (~. 15. ka BP) was associated with a caldera collapse bounded by an inward-dipping ring fault system. The ring fault system consists in a …