Search results for " DOM"
showing 10 items of 2750 documents
What monomeric nucleotide binding domains can teach us about dimeric ABC proteins
2020
The classic conceptualization of ATP binding cassette (ABC) transporter function is an ATP-dependent conformational change coupled to transport of a substrate across a biological membrane via the transmembrane domains (TMDs). The binding of two ATP molecules within the transporter's two nucleotide binding domains (NBDs) induces their dimerization. Despite retaining the ability to bind nucleotides, isolated NBDs frequently fail to dimerize. ABC proteins without a TMD, for example ABCE and ABCF, have NBDs tethered via elaborate linkers, further supporting that NBD dimerization does not readily occur for isolated NBDs. Intriguingly, even in full-length transporters, the NBD-dimerized, outward-…
Decipher the mechanisms of protein conformational changes induced by nucleotide binding through free-energy landscape analysis: ATP binding to Hsp70.
2013
ATP regulates the function of many proteins in the cell by transducing its binding and hydrolysis energies into protein conformational changes by mechanisms which are challenging to identify at the atomic scale. Based on molecular dynamics (MD) simulations, a method is proposed to analyze the structural changes induced by ATP binding to a protein by computing the effective free-energy landscape (FEL) of a subset of its coordinates along its amino-acid sequence. The method is applied to characterize the mechanism by which the binding of ATP to the nucleotide-binding domain (NBD) of Hsp70 propagates a signal to its substrate-binding domain (SBD). Unbiased MD simulations were performed for Hsp…
Interaction ofEscherichia colihemolysin with biological membranes
2001
Escherichia coli hemolysin (HlyA) is a membrane-permeabilizing protein belonging to the family of RTX-toxins. Lytic activity depends on binding of Ca2(+) to the C-terminus of the molecule. The N-terminus of HlyA harbors hydrophobic sequences that are believed to constitute the membrane-inserting domain. In this study, 13 HlyA cysteine-replacement mutants were constructed and labeled with the polarity-sensitive fluorescent probe 6-bromoacetyl-2-dimethylaminonaphthalene (badan). The fluorescence emission of the label was examined in soluble and membrane-bound toxin. Binding effected a major blue shift in the emission of six residues within the N-terminal hydrophobic domain, indicating inserti…
Human Inducible Hsp70: Structures, Dynamics, and Interdomain Communication from All-Atom Molecular Dynamics Simulations
2015
The 70 kDa human heat shock protein is a major molecular chaperone involved in de novo folding of proteins in vivo and refolding of proteins under stress conditions. Hsp70 is related to several "misfolding diseases" and other major pathologies, such as cancer, and is a target for new therapies. Hsp70 is comprised of two main domains: an N-terminal nucleotide binding domain (NBD) and a C-terminal substrate protein binding domain (SBD). The chaperone function of Hsp70 is based on an allosteric mechanism. Binding of ATP in NBD decreases the affinity of the substrate for SBD, and hydrolysis of ATP is promoted by binding of polypeptide segments in the SBD. No complete structure of human Hsp70 is…
Consumo y medio ambiente en el País Valenciano (1980-2000)
2006
At the end of the 1970s, after more than a decade of intense economic development, some marks of the mass consumption model were engraved in the Valencian society: a commercialisation system increasingly dominated by large corporations which trade in worldwide markets and use massive advertising; an animal-protein based diet, with products imported from far-away countries, with a strong presence of highly processed and packaged food and beverage; mobility based on cars; preference for owned housing, sub-urbanisation tendency, and growing energy use; «use-and-dump» culture defined by short life of products and high production of waste. This consumption model is highly homogenous and indicate…
Demographic change, tourism expenditure and life cycle behavior
2015
Abstract The paper explores how demographic changes in human populations may impact on tourism decision-making of households over their life cycle. By means of hurdle models, the study focuses respectively on the decision to travel domestically or abroad and investigates if statistically significant differences exist as to the impact of age and cohort on the decision to travel and tourism expenditure. Using a vast dataset on Italian Household Expenditures over the period 1997–2007 the empirical results show that demographic aspects do have a strong effect on the tourism behaviour of families and that cohort and age effects act differently on the decision of households to make a trip and on …
Drivers of greenhouse gas emissions in the Baltic States: A structural decomposition analysis
2014
Since the mid-1990s three Baltic States have significantly increased their per capita gross domestic product (GDP) and at the same time have managed to keep the CO2 equivalent (CO2e) emissions low. We used structural decomposition analysis to identify the drivers of change for CO2e emissions in these countries between 1995 and 2009, a period that includes the collapse of the Soviet Union, restructuring and economic growth and the great recession. The results show that final demand has been the main driving force for increasing emissions in the Baltic States and would have caused an 80%, 64% and 143% emission increase in Estonia, Latvia and Lithuania, respectively, all other factors kept con…
Stochastic equation of population dynamics with diffusion on a domain
2003
We consider Lotka-Volterra competition model with diffusion in a territorial domain with a stochastic perturbation which represents the random variations of environment conditions. We prove the existence, the uniqueness and the positivity of the solution. Moreover, the stochastic boundedness of the solution is analized.
Hilbert-Huang versus Morlet wavelet transformation on mismatch negativity of children in uninterrupted sound paradigm
2008
Background Compared to the waveform or spectrum analysis of event-related potentials (ERPs), time-frequency representation (TFR) has the advantage of revealing the ERPs time and frequency domain information simultaneously. As the human brain could be modeled as a complicated nonlinear system, it is interesting from the view of psychological knowledge to study the performance of the nonlinear and linear time-frequency representation methods for ERP research. In this study Hilbert-Huang transformation (HHT) and Morlet wavelet transformation (MWT) were performed on mismatch negativity (MMN) of children. Participants were 102 children aged 8–16 years. MMN was elicited in a passive oddball parad…
Guaranteed tuning of PID controllers for parametric uncertain systems
2004
This paper presents a methodology for tuning the controller of a parametric uncertain plant in order to guarantee the satisfaction of specifications. These specifications are defined by means of an interval reference model that represents the set of satisfactory time or frequency behaviors. The controller synthesis is then formulated as a set-inclusion problem in the frequency domain. Interval techniques for the solution of constraint satisfaction problems are applied to design fixed-structure controllers in a guaranteed manner. In particular, PID controllers are considered since they are widely used in industry.