Search results for " DOM"
showing 10 items of 2750 documents
Identification of the essential protein domains for Mib2 function during the development of the Drosophila larval musculature and adult flight muscles
2016
The proper differentiation and maintenance of myofibers is fundamental to a functional musculature. Disruption of numerous mostly structural factors leads to perturbations of these processes. Among the limited number of known regulatory factors for these processes is Mind bomb2 (Mib2), a muscle-associated E3 ubiquitin ligase, which was previously established to be required for maintaining the integrity of larval muscles. In this study, we have examined the mechanistic aspects of Mib2 function by performing a detailed functional dissection of the Mib2 protein. We show that the ankyrin repeats, in its entirety, and the hitherto uncharacterized Mib-specific domains (MIB), are important for the…
Oligodendrocyte-specific FADD deletion protects mice from autoimmune-mediated demyelination.
2010
Abstract Apoptosis of oligodendrocytes (ODCs), the myelin-producing glial cells in the CNS, plays a central role in demyelinating diseases such as multiple sclerosis and experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. To investigate the mechanism behind ODC apoptosis in EAE, we made use of conditional knockout mice lacking the adaptor protein FADD specifically in ODCs (FADDODC-KO). FADD mediates apoptosis by coupling death receptors with downstream caspase activation. In line with this, ODCs from FADDODC-KO mice were completely resistant to death receptor-induced apoptosis in vitro. In the EAE model, FADDODC-KO mice followed an ameliorated clinical di…
Dual Topology of the Hepatitis B Virus Large Envelope Protein
2001
The large (L) envelope protein of the hepatitis B virus (HBV) has the peculiar capacity to form two transmembrane topologies via an as yet uncharacterized process of partial post-translational translocation of its pre-S domain across membranes. In view of a current model that predicts an HBV-specific channel generated during virion envelope assembly to enable pre-S translocation, we have examined parameters influencing L topogenesis by using protease protection analysis of wild-type and mutant L proteins synthesized in transfected cells. We demonstrate that contrary to expectation, all determinants, thought to be responsible for channel formation, are dispensable for pre-S reorientation. In…
γ2-Adaptin, a Ubiquitin-interacting Adaptor, Is a Substrate to Coupled Ubiquitination by the Ubiquitin Ligase Nedd4 and Functions in the Endosomal Pa…
2008
gamma2-Adaptin is a putative member of the clathrin adaptor protein family with unknown physiological function. We previously reported that gamma2-adaptin acts as a ubiquitin receptor by virtue of its ubiquitin-interacting motif. Here we demonstrate that this motif mediates a specific physical interaction with the ubiquitin ligase Nedd4 and promotes ubiquitination of gamma2-adaptin. By mapping regions of Nedd4 involved in binding to gamma2-adaptin, we identified its C2 domain to be essential, whereas the WW and HECT domains are dispensable. Consistent with this, we uncovered that the C2 domain of Nedd4 is ubiquitinated itself and as such is recruited by the ubiquitin-interacting motif of ga…
Towards the observation of Territorial Energy Systems : a geographical design approach for energy territorialisation
2014
Having an effect on a system calls for a thorough knowledge of it. The main goal of this research is toprovide a general framework for the interpretation of geographic information, as well as a methodologicalframework to understand the interrelations between territory and energy in the context of a territorialobservatory. A literature review of energy planning on the one hand and spatial planning on the other revealssimilar developments in the two fields, in particular in terms of decentralisation and environmental concerns.The change of geographical scale chosen for the analysis brings new possibilities for public intervention. Inthis context, therefore, local authorities have a key role t…
Robot Shift from Industrial Production to Social Reproduction
2015
This chapter analyses people’s attitudes towards the use of robots in the different domains of life and, specifically, in the domain of social reproduction. The analysis is based on Eurobarometer 382 “Public Attitudes towards Robots” data (N = 26,751), which was carried out among EU citizens aged 15 and over in 27 member states in 2012. The results of the study show that on average European perceptions of robots are positive and permissive. The life domains in which robots have already been used for a long time (e.g. space exploration, manufacturing, military and security business, search and rescue work) turn out to be the most popular areas for the further penetration of robots. The least…
Broken rotor bars detection via Park's vector approach based on ANFIS
2014
Many attempts have been made on fault diagnosis of induction motors based on frequency and time domain analysis of stator current. In this paper, first the Park's vector transformation and frequency analysis for fault detection of induction motors are introduced. Then a smart approach using Adaptive Neuro Fuzzy Inference System (ANFIS) is proposed. This approach uses the time domain features derived from the Park's vector transformation of stator current. By the proposed method, a partial break including 5 mm crack on a bar, one broken bar and two broken bars using experimental data are investigated. It will be shown that features derived from Park's vector compared to features obtained fro…
Specifying Patterns for Mobile Application Domain Using General Architectural Components
2005
Software companies adopt patterns as a means to improve architecture and design practices. During recent years, the application of patterns has extended from general software applications to specific problem domains. In a new domain, suitable patterns fitting to the essential design problems in the new context need to be identified. In this paper, we introduce a general architectural model of mobile applications, which can be used to identify and organise essential patterns in mobile-application design process. This model is employed to construct a high-level architecture of a particular application. For each component of the architecture, the model may suggest candidate patterns that can b…
Bending-Shear interaction domains for externally prestressed concrete girders
2013
In prestressed concrete structures, the evaluation of the safety level is generally carried out by separating the bending moment strength and the shear force capacity. Actually interaction between bending moment (M) and shear force (V) can have significant consequences on evaluations in service life, especially when the ultimate limit state (ULS) is considered. In this paper, the M-V interaction is addressed for prestressed concrete girders, in the cases of both bonded and unbonded prestressing tendons. It can be demonstrated, by drawing the interaction domains (M-V), that a significant reduction of the safety level has to be considered when shear force is evaluated together with bending mo…
A frequency compensation algorithm of four-wheel coherence random road
2013
Published version of an article in the journal: Mathematical Problems in Engineering. Also available from the publisher at: http://dx.doi.org/10.1155/2013/986584 Open access The road surface roughness is the main source of kinematic excitation of a moving vehicle, which has an important influence on vehicle performance. In recent decades, random road models have been widely studied, and a four-wheel random road time domain model is usually generated based on the correlation of the four-wheel input, in which a coherence function is used to describe the coherence of the road input between the left and right wheels usually. However, during our research, there are some conditions that show that…