Search results for " E.P.E."
showing 10 items of 5612 documents
Baculovirus-mediated immediate-early gene expression and nuclear reorganization in human cells
2007
Baculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), has the ability to transduce mammalian cell lines without replication. The general objective of this study was to detect the transcription and expression of viral immediate-early genes in human cells and to examine the interactions between viral components and subnuclear structures. Viral capsids were seen in large, discrete foci in nuclei of both dividing and non-dividing human cells. Concurrently, the transcription of viral immediate-early transregulator genes (ie-1, ie-2) and translation of IE-2 protein were detected. Quantitative microscopy imaging and analysis showed that virus transduction altered the size of …
The human autoantigen La/SS-B accelerates herpes simplex virus type 1 replication in transfected mouse 3T3 cells.
1998
SUMMARY Permanently transfected mouse cell lines which expressed different levels of the human autoantigen La/SS-B were infected with different strains of herpes simplex virus type 1, including the strains ANG, HSZP, 17syn+ and HFEM. During infection the localization of the human La protein was followed using an anti-La MoAb, which recognized only the human La protein but did not cross-react with either the endogenous mouse La protein or any viral encoded protein. After infection La protein was transported from the nucleus to the cytoplasm. The time course of translocation was dependent on the amount of human La protein expressed in the respective cell line. Moreover, acceleration of viral …
Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme
1997
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…
Event-related potentials to task-irrelevant changes in facial expressions
2009
Abstract Background Numerous previous experiments have used oddball paradigm to study change detection. This paradigm is applied here to study change detection of facial expressions in a context which demands abstraction of the emotional expression-related facial features among other changing facial features. Methods Event-related potentials (ERPs) were recorded in adult humans engaged in a demanding auditory task. In an oddball paradigm, repeated pictures of faces with a neutral expression ('standard', p = .9) were rarely replaced by pictures with a fearful ('fearful deviant', p = .05) or happy ('happy deviant', p = .05) expression. Importantly, facial identities changed from picture to pi…
El trabajo cedido en el sistema de empleo español. Una aproximación sociológica desde las racionalidades y vivencias de los trabajadores cedidos
2020
Desde los años 80 han proliferado nuevas formas atípicas de empleo en el Estado Español. En este contexto, se legaliza el trabajo cedido en 1994. Según los últimos datos del Anuario de Estadísticas Laborales, el número de trabajadores cedidos es de 708.600 en 2017, habiendo alcanzado la cifra de 1.678.668 en 2009. En el ámbito académico español se ha examinado el trabajo cedido desde una perspectiva jurídica y económica. En menor medida encontramos algún estudio sobre la precariedad de las personas que tienen este estatus. No obstante, en el ámbito europeo encontramos varios estudios que analizan sus características, trayectorias laborales y condiciones de empleo. Glaymann(2005:20) plantea …
Preparing an emotionally expressive vocal performance
2018
This paper explores the processes, strategies, and methods used when preparing an expressive vocal performance from the point-of-view of the artist. The study tracked the development and performance preparation of 13 university students studying classical voice performance or music education. Participants were asked to choose one unfamiliar piece from their repertoire and complete three surveys administered at the beginning, middle, and end of their study term. Each survey was geared toward the participants’ level of preparation and pertained to their approaches for learning new repertoire, the application of constructive criticism from peers and instructors, and experiences during and afte…
«Telemaco»: una ricerca sperimentale in prospettiva Student Voice
2017
La ricerca «Telemaco» è stata svolta per promuovere una partecipazione attiva e responsabile degli studenti universitari alla didattica universitaria. A tal fine, si è ritenuto opportuno preparare tale partecipazione mediante l’applicazione di due metodi d’intervento con cui migliorare il loro senso di autoefficacia, la loro capacità di gestire l’ansia da prestazione e l’immagine del loro «sé» attuale: la scrittura espressiva e il tutoraggio tra pari. I risultati dimostrano l’efficacia di questi due metodi sulle tre competenze prese in esame. Valorizzare la voce scritta e orale degli studenti forma in loro una visione più democratica dell’insegnamento universitario, attiva processi virtuosi…
Who Controls Who? Embodied Control Within Human–Technology Choreographies
2017
In this article we explore issues of embodied control that relate to current and future technologies in which body movements function as an instrument of control. Instead of just seeing ourselves in control, it is time to consider how these technologies actually control our moving bodies and transform our lived spaces. By shifting the focus from devices to choreographies among devices, we perform a theoretical analysis of the multidimensional aspects that reside within embodied interaction with technology. We suggest that it is beneficial to acknowledge and reformulate the phenomena of embodied control that go beyond the instrumental user-to-device control scheme. Drawing upon the phenomeno…
Marketing y responsabilidad. Una aproximación ética desde René Girard
2016
La presente tesis tiene como objeto analizar la ética aplicada al marketing desde la perspectiva de la responsabilidad bajo el presupuesto de la hermenéutica crítica. La ética del marketing supone el descubrimiento y análisis de los principios, valores y fundamentos éticos en el campo del marketing y su función dentro de las organizaciones, así como las responsabilidades del mismo en sus ámbitos de acción e influencia. Para ello, hemos respondido en la primera parte, a qué es el marketing, por cuanto una ética del mismo vendrá necesariamente determinada por su concepción y hemos determinado el deseo, la identidad y la confianza como elementos centrales para el marketing del siglo XXI. La se…
Donación y deliberación: El lugar de la caridad en la ética empresarial
2010
El artículo analiza algunas aportaciones de la encíclica Caritas in veritate (CiV) a la ética social contemporánea. Una aportación de gran valor hermenéutico porque integra tres categorías centrales en la ética contemporánea: globalización, deliberación y donación. Dedica especial atención al concepto de caridad en la primera y tercera parte del trabajo, primero analizando la presencia de este concepto en la ética social reciente en términos de des-privatización, después muestra la emergencia de la caridad en la ética empresarial. Entre las aportaciones que realiza la encíclica a la ética empresarial nos hemos detenido en las que afectan a las formas de gestión organizativa y que hemos llam…