Search results for " EXPERIMENTAL"
showing 10 items of 3530 documents
Heat Shock Protein Vaccines Against Cancer
1993
Vaccination of mice with heat shock proteins (HSPs) derived from a tumor makes the mice resistant to the tumor from which the HSP was obtained. This phenomenon has been demonstrated with three HSPs--gp96, hsp90, and hsp70. Vaccination with HSPs also elicits antigen-specific cytotoxic T lymphocytes (CTLs). The specific immunogenicity of HSPs derives apparently, not from the HSPs per se, but from the peptides bound to them. These observations provide the basis for a new generation of vaccines against cancer. The HSP-based cancer vaccines circumvent two of the most intractable hurdles to cancer immunotherapy. One of them is the possibility that human cancers, like cancers of experimental anima…
Comparative antitumor effect among GM-CSF, IL-12 and GM-CSF+IL-12 genetically modified tumor cell vaccines.
2013
Genetically modified cells have been shown to be one of the most effective cancer vaccine strategies. An evaluation is made of the efficacy of both preventive and therapeutic antitumor vaccines against murine melanoma, using C57BL/6 mice and irradiated B16 tumor cells expressing granulocyte and macrophage colony-stimulating factor (GM-CSF), interleukin-12 (IL-12) or both. Tumor was transplanted by the injection of wild-type B16 cells. Tumor growth and survival were measured to evaluate the efficacy of vaccination. Specific humoral response and immunoglobulin G (IgG) switch were evaluated measuring total IgG and IgG1 and IgG2a subtypes against tumor membrane proteins of B16 cells. In prevent…
Cannabinoid CB1 receptors regulate neuronal TNF-α effects in experimental autoimmune encephalomyelitis.
2011
Abstract Cannabinoid CB1 receptors (CB1Rs) regulate the neurodegenerative damage of experimental autoimmune encephalomyelitis (EAE) and of multiple sclerosis (MS). The mechanism by which CB1R stimulation exerts protective effects is still unclear. Here we show that pharmacological activation of CB1Rs dampens the tumor necrosis factor α (TNFα)-mediated potentiation of striatal spontaneous glutamate-mediated excitatory postsynaptic currents (EPSCs), which is believed to cogently contribute to the inflammation-induced neurodegenerative damage observed in EAE mice. Furthermore, mice lacking CB1Rs showed a more severe clinical course and, in parallel, exacerbated alterations of sEPSC duration af…
Composition as identity and plural Cantor's theorem
2016
In this paper, I argue that the thesis of Composition as Identity blocks the plural version of Cantor’s Theorem, and that this in turn has implications for our use of Cantor’s theorem in metaphysics. As an example, I show how this result blocks a recent argument by Hawthorne and Uzquiano, and might be turned around to become an abductive argument for Composition as Identity
Analisi Sperimentale del Comportamento Ciclico di Elementi in Calcestruzzo a Bassa Resistenza Confinati con FRCM
2013
Vengono riportati i principali risultati di una campagna sperimentale finalizzata alla caratterizzazione della risposta sotto carichi assiali ciclici di colonne di calcestruzzo a bassa resistenza con sezione circolare, quadrata e rettangolare, rinforzate con fibre di carbonio immerse in matrice cementizia (CFRCM). Sono state condotte prove di compressione assiale su 30 campioni di altezza 600 mm, 10 a sezione cilindrica di diametro 300 mm, 10 quadrata di lato 200 mm, e 10 rettangolare di lato 200x400 mm. Le prove sono finalizzate a indagare gli effetti del carico ciclico, della forma della sezione, del raggio di curvatura degli spigoli e del numero di strati di tessuto di rinforzo sul legam…
Stimulation of peroxisomal palmitoyl-CoA oxidase activity by ciprofibrate in hepatic cell lines: comparative studies in Fao, MH1C1 and HepG2 cells.
1993
The response of two rat cell lines, Fao and MH1C1, and one human cell line, HepG2, to the peroxisome proliferator ciprofibrate, was studied. Using a fluorometric assay for palmitoyl-CoA oxidase, the dose- and time-dependent increase of this enzymatic activity was determined. From the lowest concentration (100 microM) stimulation is evident in the two rat cell lines. In the Fao line, the activity was stimulated reaching a seven-fold increase over the control level at 250 microM after 72 h of treatment. In the MH1C1 line, the maximum stimulation, four- to five-fold, was obtained at 250 and 500 microM after 72 h. In the HepG2 cell line, activity increased two-fold at 250 microM after 72 h reac…
Loss of response of carnitine palmitoyltransferase I to okadaic acid in transformed hepatic cells
1998
The specific activity of carnitine palmitoyltransferase I (CPT-I) was similar in mitochondria isolated from rat Fao and human HepG2 hepatoma cells and from rat hepatocytes, but almost twofold higher in permeabilized hepatoma cells than in permeabilized hepatocytes. Short-term exposure to okadaic acid induced a ca. 80% stimulation of CPT-I in hepatocytes, whereas no significant response of the enzyme from hepatoma cells was evident. Thus, the high CPT-I activity displayed by hepatoma cells may be reached by hepatocytes upon challenge to okadaic acid. Reconstitution experiments with purified mitochondrial and cytoskeletal fractions showed that the cytoskeleton of hepatocytes produced a more r…
DEVELOPMENT OF CAREER MANAGEMENT SKILLS OF NEW PODOLOGISTS FOR WORK IN A MULTICULTURAL ENVIRONMENT
2017
Because of the affections of globalization process and because of the changes in social-political situation in world, multicultural environment in Latvia is also changing. Latvia becomes more and more attractive for foreign citizens from Europe the same as for citizens from elsewhere, that choose to settle down here permanently or only for a period of time. That is considerable challenge for education and healthcare.
 Workers in field of medicine, and podologs as well, must guide their professional carreer development in mutable multicultural environment. That is why needs of joung specialists are changing too-with knowledge, skills and competencies necessary in proffession, specialist…
The CO-releasing molecule CORM-3 protects against articular degradation in the K/BxN serum transfer arthritis model.
2010
Contains fulltext : 89015.pdf (Publisher’s version ) (Closed access) Carbon monoxide-releasing molecules can counteract inflammatory responses. The aim of this study was to investigate whether tricarbonylchloro(glycinate)ruthenium (II) (CORM-3) is able to control the effector phase of experimental arthritis. Arthritis was induced in C57Black-6 mice by an intraperitoneal injection of serum from arthritic K/BxN mice. CORM-3 was administered intraperitoneally at 10 mg/kg/day (5 mg/kg twice a day) from days 0 to 10 and animals were sacrificed on day 11. Serum levels of osteocalcin and prostanoids were measured by enzyme-linked immunosorbent assay and radioimmunoassay. Gene expression was determ…
Influence of age on osteoarthritis progression after anterior cruciate ligament transection in rats.
2013
Abstract The anterior cruciate ligament transection (ACLT) model of osteoarthritis (OA) in young rats is widely used to study the pathogenesis of OA and possible treatment approaches. As aging is a key factor in the progression of this condition, it is hypothesized that animals may vary in their responses to ACLT according to their age. The histopathological features of young (2 month-old) and middle-aged (12 month-old) rats in the presence or absence of ACLT were compared. The results indicated that moderate degradative changes can be detected in the knee joints of sham-operated middle-aged rats compared with young animals. After ACLT, cartilage degradation was significantly higher in midd…