Search results for " EXTENSION"
showing 10 items of 272 documents
Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi.
2016
Cold acclimation is a critical physiological adaptation for coping with seasonal cold. By increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. In insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic analyses in Drosophila virilis and D. montana revealed candidate genes for cold acclimation by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate synthase (Inos), in cold tolerance in D. montana using an RNAi approach. D. montana has a circumpolar distribution and overwinters…
Generation of a DNA microarray for determination of E6 natural variants of human papillomavirus type 16.
2003
Infection with high-risk types of human papillomavirus (HPV) is necessary for the development of cervical cancer. However, the majority of the HPV infections are efficiently cleared by the immune system and only a minority persist and induce the development of malignant lesions. Several studies provided evidence that intratype genetic variations are implicated in determining the clinical outcome of HPV infections. In this study, we describe a DNA chip based on arrayed primer extension (APEX) for the analysis of the natural variants of HPV16, the most frequently detected type in cervical cancer world-wide. We show that HPV16 E6 variants are detected efficiently by APEX. In addition, APEX is …
Comparative analysis of short tandem repeats and single nucleotide polymorphisms on the Y-chromosome in Germans, Chinese and Thais.
2003
We have typed genomic DNA samples from 95 individuals from Western Germany, 78 individuals from Bangkok/Thailand and 56 individuals from Chengdu/China for 11 Y-chromosomal diallelic polymorphisms and eight short tandem repeat (STR) systems. For single nucleotide polymorphism (SNP) analysis, a rapid method was applied using the single base extension technology (minisequencing) in combination with capillary electrophoresis. PCR products for SRY-8299, Tat, SRY2627, 92R7, SRY1532, M9, M13, M17/M19 and M20 were pooled and used as templates for the commercially available SNaPshot kit. In addition to these ten SNPs we also tested the Y-chromosomal diallelic Alu repeat insertion DYS287 (YAP) by aga…
A multiplex assay with 52 single nucleotide polymorphisms for human identification.
2006
A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We established a highly sensitive and reproducible SNP-typing method with amplification of all 52 DNA fragments in one PCR reaction followed by detection of the SNPs with two single base extension reactions analysed using CE. The amplicons ranged from 59 to 115 bp in length. Complete SNP profiles were obtained from 500 pg DNA. The 52 loci were efficiently amplified from degraded samples where previously on…
Extensions of cocycles for hyperfinite actions and applications
1997
Given a countable, hyperfinite, ergodic and measure-preserving equivalence relationR on a standard probability space (X, ℬ, μ) and an elementW of the normalizerN (R) ofR, we investigate the problem of extendingR-cocycles to\(\bar R\), where\(\bar R\) is the relation generated byR andW. As an application, we obtain that for a Bernoulli automorphism the smallest family of natural factors in sense of [6] consists of all factors. Given an automorphism which is embeddable in a measurable flow and a compact, metric group, we show that for a typical cocycle we cannot lift the whole flow to the centralizer of the corresponding group extension.
Zu einem Satz von Isaacs �ber das Casus-Irreducibilis Ph�nomen
2000
Let \(\Omega \) be a field (of characteristic 0). A prime p is called “bose” (naughty) if \(\Omega \) contains all p-th roots of unity. In this paper the theorem is proved: Let K be an admissible subfield of \(\Omega \) (i.e. for each prime p K contains all p-th roots of unity lying in \(\Omega \)), a an algebraic element of \(\Omega /K\) which is contained in a repeated radical extension of K lying in \(\Omega \). Furthermore let the normal hull L of a over K be contained in \(\Omega \). Then all prime divisors of \(\mid L : K \mid \) are naughty (and L is a repeated radical extension of K with naughty prime exponents). This result generalises a theorem of Isaacs [1] who treats the case \(…
Approximation of functions over manifolds : A Moving Least-Squares approach
2021
We present an algorithm for approximating a function defined over a $d$-dimensional manifold utilizing only noisy function values at locations sampled from the manifold with noise. To produce the approximation we do not require any knowledge regarding the manifold other than its dimension $d$. We use the Manifold Moving Least-Squares approach of (Sober and Levin 2016) to reconstruct the atlas of charts and the approximation is built on-top of those charts. The resulting approximant is shown to be a function defined over a neighborhood of a manifold, approximating the originally sampled manifold. In other words, given a new point, located near the manifold, the approximation can be evaluated…
On conditional probabilities and their canonical extensions to Boolean algebras of compound conditionals
2023
In this paper we investigate canonical extensions of conditional probabilities to Boolean algebras of conditionals. Before entering into the probabilistic setting, we first prove that the lattice order relation of every Boolean algebra of conditionals can be characterized in terms of the well-known order relation given by Goodman and Nguyen. Then, as an interesting methodological tool, we show that canonical extensions behave well with respect to conditional subalgebras. As a consequence, we prove that a canonical extension and its original conditional probability agree on basic conditionals. Moreover, we verify that the probability of conjunctions and disjunctions of conditionals in a rece…
Assessing farming eco-efficiency: a Data Envelopment Analysis approach.
2010
This paper assesses farming eco-efficiency using Data Envelopment Analysis (DEA) techniques. Eco-efficiency scores at both farm and environmental pressure-specific levels are computed for a sample of Spanish farmers operating in the rain-fed agricultural system of Campos County. The determinants of eco-efficiency are then studied using truncated regression and bootstrapping techniques. We contribute to previous literature in this field of research by including information on slacks in the assessment of the potential environmental pressure reductions in a DEA framework. Our results reveal that farmers are quite eco-inefficient, with very few differences emerging among specific environmental …
Brand Extension: The Moderating Role of the Category to which the Extension Belongs to Be Added
2008
This article examines an evaluation of brand extension from a consumption perspective. In this perspective, the most relevant entity becomes the product, and the choice vector becomes the consumption context. In an experimental design applied to foodstuff, this article reports a study that manipulates the brand range. The study confirms the importance of the consumption context to evaluate brand extension. Also, the results show that the effects of the context fit and the typicality are more significant when the various products of the brand are sensitive to the consumption context. On the other hand, the effects of the association fit and typicality are also more significant when the produ…