Search results for " Experimental"
showing 10 items of 3530 documents
Targeting of the transcription factor STAT4 by antisense phosphorothioate oligonucleotides suppresses collagen-induced arthritis
2007
Abstract The transcription factor STAT4 mediates signals of various proinflammatory cytokines, such as IL-12, IL-15, and IL-23, that initiate and stabilize Th1 cytokine production. Although Th1 cytokine production has been suggested to play a major pathogenic role in rheumatoid arthritis, the role of STAT4 in this disease is poorly understood. In this study, we demonstrate a key functional role of STAT4 in murine collagen-induced arthritis (CIA). In initial studies we found that STAT4 expression is strongly induced in CD4+ T cells and to a lesser extent in CD11b+ APCs during CIA. To analyze the role of STAT4 for arthritis manifestation, we next investigated the outcome of interfering with S…
H2-M polymorphism in mice susceptible to collagen-induced arthritis involves the peptide binding groove.
1996
The ability to develop type II collagen (CII)-induced arthritis (CIA) in mice is associated with the major histocompatibilityI-A gene and with as yet poorly defined regulatory molecules of the major histocompatibility complex (MHC) class II antigen processing and presentation pathway. H2-M molecules are thought to be involved in the loading of antigenic peptides into the MHC class II binding cleft. We sequencedH2-Ma, H2-Mb1, andH2-Mb2 genes from CIA-susceptible and-resistant mouse strains and identified four differentMa andMb2 alleles and three differentMb1 alleles defined by polymorphic residues within the predicted peptide binding groove. Most CIA-resistant mouse strains share commonMa, M…
Functional characterization of osteosarcoma cell lines provides representative models to study the human disease
2011
Cancer cell lines represent in vitro models for studying malignancies, general cell biology, drug discovery and more. Whether they can be considered as exact representative models of the parental tumors remains uncertain given the acquisition of additional ex vivo changes of the cells and the lack of tissue architecture and stroma. Previously, within the EuroBoNeT consortium, we characterized a collection of bone sarcoma cell lines on genomic and proteomic level. Here, we address the phenotypical and functional characterization of the unique set of osteosarcoma cell lines (n=19) in vitro and in vivo. For functional analysis of differentiation capacity, cells were stimulated towards osteobla…
Non-conventional forms of HLA-B27 are expressed in spondyloarthritis joints and gut tissue
2016
Objectives Human leukocyte antigen (HLA)-B27 (B27) is the strongest genetic factor associated with development of Ankylosing Spondylitis and other spondyloarthropathies (SpA), yet the role it plays in disease pathogenesis remains unclear. We investigated the expression of potentially pathogenic non-conventional heavy chain forms (NC) of B27 in synovial and intestinal tissues obtained from SpA patients. We also determined the presence of NC-B27 in joints, lymphoid and gastrointestinal tissue from B27 transgenic (TG1) rats with M.tuberculosis-induced SpA. Methods Expression of NC-B27 in human SpA joints and gut and in (21-3 × 283-2)F1 HLA-B27/Huβ2m rat tissue was determined by immunohistochem…
Treatment with a CO-releasing molecule (CORM-3) reduces joint inflammation and erosion in murine collagen-induced arthritis.
2008
Contains fulltext : 70589.pdf (Publisher’s version ) (Closed access) OBJECTIVE: CO-releasing molecules (CO-RMs) are a novel class of anti-inflammatory agents. We have examined the possible therapeutic effects of CORM-3 in collagen-induced arthritis (CIA). METHODS: Arthritis was induced in DBA-1/J mice by type II collagen. Animals were treated with CORM-3 (5 and 10 mg/kg/day, intraperitoneally) or the inactive compound iCORM-3 (10 mg/kg/day, intraperitoneally) unable to release CO, from days 22 to 31. Production of anti-type II collagen antibodies, cytokines and cartilage olimeric matrix protein (COMP) was evaluated by enzyme-linked immunosorbent assay, and prostaglandin E(2) (PGE(2)) by rad…
In Vitro Study on Bone Heating during Drilling of the Implant Site: Material, Design and Wear of the Surgical Drill
2020
Objective: An in vitro study was made to compare mean thermal variation according to the material, design and wear of the surgical drills used during dental implant site preparation. Material and methods: Three study groups (stainless steel drills with straight blades
Emotions of music listening in Finland and in India : Comparison of an individualistic and a collectivistic culture
2020
Music is appreciated for emotional reasons across cultures, but knowledge on the cross-cultural similarities and differences of music-evoked emotions is still sparse. The current study compared music-evoked emotions in Finland and in India, contextualizing them within the perceived psychological functionality of music in an individualistic versus collectivistic culture. Participants ( N = 230) answered an online survey on music-evoked emotions and related personal meanings. A mixed-method approach using factor analysis and qualitative content analysis was used to identify the concepts for cross-cultural comparison. Results show that both cultures value music for positive emotional experien…
Brechas de las políticas públicas y la intervención profesional: Familismo y cuidados en salud mental
2021
La atención en salud mental ha sufrido grandes cambios epistemológicos y prácticos, representando un largo y complejo proceso histórico y cultural. En la actualidad, el aumento de la prevalencia de los trastornos mentales y la limitada disponibilidad de recursos hace que la atención a la salud mental recaiga sobre la familia. Esta se ha constituido como principal proveedor de atención y apoyo continuo, asumiendo así la carga derivada del cuidado, especialmente la persona que ejerce el rol de cuidadora principal. A través del compendio de publicaciones científicas, la finalidad de esta tesis doctoral es conocer y explicar de manera crítica e integral los desafíos y barreras que envuelven el …
Narrative Tensions in Strained Junior Elite Performers’ Experiences of Becoming Elite Performers
2021
Contextualized within narrative theory and the field of talent identification and development systems (TIDS), this interview study examined strained junior elite performers’ experiences of becoming elite performers while participating in prestigious national TIDS. The study explored how junior elite performers perceive and negotiate their personal narratives of becoming within a cultural master narrative of being. The focus is on how the quality of person-environment interaction, characterized by narrative alignment or tensions, relates to perceptions of identity, agency, and physical and mental health. We purposefully recruited eight participants (Mage = 17.31, SD = 0.9) from a previously …
Peripersonal Visuospatial Abilities in Williams Syndrome Analyzed by a Table Radial Arm Maze Task
2020
Williams syndrome (WS) is a genetic deletion syndrome characterized by severe visuospatial deficits affecting spatial exploration and navigation abilities in extra-personal space.To date, little is known about spatial elaboration and reaching abilities in the peripersonal space in individuals with WS. The present study is aimed at evaluating the visuospatial abilities in individuals with WS and comparing their performances with those of mental age-matched typically developing (TD) children by using a highly sensitive ecological version of the Radial Arm Maze (table RAM). We evaluated 15 individuals with WS and 15 TD children in two different table RAM paradigms: the free-choice paradigm, ma…