Search results for " Farm"

showing 10 items of 2009 documents

Development of a simple, biocompatible and cost-effective Inulin-Diethylenetriamine based siRNA delivery system

2015

Small interfering RNAs (siRNAs) have the potential to be of therapeutic value for many human diseases. So far, however, a serious obstacle to their therapeutic use is represented by the absence of appropriate delivery systems able to protect them from degradation and to allow an efficient cellular uptake. In this work we developed a siRNA delivery system based on inulin (Inu), an abundant and natural polysaccharide. Inu was functionalized via the conjugation with diethylenetriamine (DETA) residues to form the complex Inu-DETA. We studied the size, surface charge and the shape of the Inu-DETA/siRNA complexes; additionally, the cytotoxicity, the silencing efficacy and the cell uptake-mechanis…

3003Small interfering RNAJHH6CellPharmaceutical ScienceEndocytosisCell LineIn vivoCell Line TumormedicinePolyaminesGene silencingHumansMicropinocytosisRNA Small InterferingCytotoxicityChemistry16HBEInulinEndocytosisDiethylenetriamine (DETA)Cell biologyInu-DETA copolymermedicine.anatomical_structureBiochemistryCytoplasmSettore CHIM/09 - Farmaceutico Tecnologico ApplicativosiRNA16HBE; Diethylenetriamine (DETA); Inu-DETA copolymer; Inulin; JHH6; siRNA; 3003E2F1 Transcription Factor
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PEG 400/Cerium Ammonium Nitrate Combined with Microwave-Assisted Synthesis for Rapid Access to Beta-Amino Ketones. An Easy-to-Use Protocol for Discov…

2018

Compound libraries are important requirement in target-based drug discovery. In the present work, a small focused compound library based on β-aminoketone scaffold has been prepared combining microwave-assisted organic synthesis (MAOS) with polymer-assisted solution phase synthesis (PASPS) and replacing reaction workup standard purification procedures with solid phase extraction (SPE). Specifically, the effects of solvent, such as dioxane, dimethylformamide (DMF), polyethylene glycol 400 (PEG 400), temperature, irradiation time, stoichiometric ratio of reagents, and catalysts (HCl, acetic acid, cerium ammonium nitrate (CAN)) were investigated to maximize both conversion and yield. The optimi…

3003Transcription FactorPharmaceutical ScienceNitratePolyethylene Glycol01 natural sciencesPolyethylene GlycolsPolymer-assisted solution phase synthesiAnalytical Chemistrychemistry.chemical_compoundDrug DiscoveryMannich reactionSolid phase extractionMicrowavesβ-aminoketonesCeriumKetonesKetoneDNA-Binding ProteinsSolventCeriumChemistry (miscellaneous)Molecular MedicineDimethylformamideMicrowave-assisted organic synthesiMannich reaction; β-aminoketones; microwave-assisted organic synthesis; polymer-assisted solution phase synthesis; solid phase extraction; drug discoveryDNA-Binding ProteinBacterial Proteinchemistry.chemical_elementPolyethylene glycol010402 general chemistryArticlelcsh:QD241-441Bacterial Proteinslcsh:Organic chemistryΒ-aminoketonePhysical and Theoretical ChemistrySolid phase extractionpolymer-assisted solution phase synthesisPEG 400Nitrates010405 organic chemistryDrug Discovery3003 Pharmaceutical ScienceOrganic ChemistrySettore CHIM/08 - Chimica FarmaceuticaCombinatorial chemistry0104 chemical scienceschemistryYield (chemistry)microwave-assisted organic synthesisOrganic synthesisMicrowaveTranscription FactorsMolecules
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Gold nanostar–polymer hybrids for siRNA delivery: Polymer design towards colloidal stability and in vitro studies on breast cancer cells

2017

To overcome the low bioavailability of siRNA (small interfering RNA) and to improve their transfection efficiency, the use of non-viral delivery carriers is today a feasible approach to transform the discovery of these incredibly potent and versatile drugs into clinical practice. Polymer-modified gold nanoconstructs (AuNCs) are currently viewed as efficient and safe intracellular delivery carriers for siRNA, as they have the possibility to conjugate the ability to stably entrap and deliver siRNAs inside cells with the advantages of gold nanoparticles, which can act as theranostic agents and radiotherapy enhancers through laser-induced hyperthermia. In this study, AuNCs were prepared by coat…

3003siRNA deliverySmall interfering RNAPolymersMetal NanoparticlesPharmaceutical ScienceGold Colloid02 engineering and technologyPolyethylene Glycol01 natural sciencesPolyethylene GlycolsGold Colloidchemistry.chemical_compoundDrug Delivery SystemsMCF-7 CellDrug StabilityCoatingRNA Small InterferingPolymerDrug Carrierchemistry.chemical_classificationDrug CarriersTumorLipoic acidGold nanostarPolymer021001 nanoscience & nanotechnologyColloidal goldMCF-7 Cells0210 nano-technologyDrug carrierHydrophobic and Hydrophilic InteractionsBreast NeoplasmHumanBiological AvailabilityReproducibility of ResultBreast NeoplasmsNanotechnologyPolyethylene glycolengineering.materialSmall InterferingTransfection010402 general chemistryCell LineHydrophobic and Hydrophilic InteractionMetal NanoparticleCell Line TumorAmphiphileHumansGene SilencingParticle SizeGold nanostarsReproducibility of ResultsGold nanostars; Lipoic acid; MCF-7; PEG; PHEA; siRNA delivery; Biological Availability; Breast Neoplasms; Cell Line; Tumor; Drug Carriers; Drug Delivery Systems; Drug Stability; Gene Silencing; Gold; Gold Colloid; Humans; Hydrophobic and Hydrophilic Interactions; MCF-7 Cells; Metal Nanoparticles; Particle Size; Polyethylene Glycols; Polymers; RNA; Small Interfering; Reproducibility of Results; Transfection; 3003PHEAPEG0104 chemical scienceschemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoengineeringRNAGoldMCF-7Drug Delivery SystemInternational Journal of Pharmaceutics
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Medición de la tensión arterial: método auscultatorio

2010

A través de este método se mide la tensión arterial a partir de la ausculatción distal a la arteria comprimida por el esfigmomanómetro (con frecuencia la arteria humeral o braquial).

3213.11 Ciències MédiquesExploración clínicofuncionalcardiopatíasauscultacióntensión arterialFisioterapia3213.11- Farmacia Medicina
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Realización de un electrocardiograma

2010

Procedimiento a realizar para obtener un registro electrocardiográfico. Preparación del paciente y del electrocardiógrafo.

3213.11 Ciències MédiquesExploración clínicofuncionalcardiopatíaselectrocardiografía básicaFisioterapia3213.11- Farmacia Medicina
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Prueba de esfuerzo: protocolo Bruce

2010

La prueba de esfuerzo continúa siendo una de las pruebas complementarias de mayor utilidad para el diagnóstico, pronóstico y protocolización del tratamiento de los pacientes con cardiopatía. Consiste en la realización de un esfuerzo bajo control médico, para valorar la respuesta cardiovascular a ese esfuerzo y poner evidencia alteraciones que no se manifiestan en reposo.

3213.11 Ciències MédiquesExploración clínicofuncionalcardiopatíasprueba de esfuerzoergometríaprotocolo BruceFisioterapia3213.11- Farmacia Medicina
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Medición de la tensión arterial: método palpatorio

2010

Mediante este método se mide la tensión arterial a partir de la palpación del pulso de una arteria distal al punto de compresión por el esfigmomanómetro (con frecuencia la arteria radial).

3213.11 Ciències MédiquesExploración clínicofuncionalcardiopatíastensión arterialFisioterapia3213.11- Farmacia Medicina
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Medición de la tensión arterial: método oscilométrico

2010

El método oscilómetrico (Oscilometría de Pachon o de Recklinghausen) recoge directamente las oscilaciones de las paredes arteriales en el mismo lugar de la compresión. Al igual que para los métodos auscultatorio y palpatorio, utiliza un esfigmomanómetro, y por lo tanto, se debe comprobar la anchura del manguito y su correcta colocación en el brazo.

3213.11 Ciències MédiquesExploración clínicofuncionalcardiopatíastensión arterialFisioterapia3213.11- Farmacia Medicina
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Fisioterapia en la fase de convalecencia: fase de esfuerzo

2010

Fisioterapia en la rehabilitación cardíaca: fase de convalecencia o fase II. En la sesión de entrenamiento, la fase de esfuerzo o endurecimiento pretende aumentar la capacidad funcional, la capacidad máxima de captación de oxígeno y la capacidad aeróbica máxima del paciente.

3213.11 Ciències MédiquesFisioterapia3213.11- Farmacia MedicinaFisioterapiarehabilitación cardiacacardiópatasejercicio físicofrecuencia cardíaca de entrenamientocasos clínicos
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Fisioterapia en la fase de convalecencia: preparación

2010

Fisioterapia en la rehabilitación cardíaca: fase de convalecencia o fase II. Preparación del espacio y del paciente para realizar la sesión de entrenamiento.

3213.11 Ciències MédiquesFisioterapia3213.11- Farmacia MedicinaFisioterapiarehabilitación cardiacacardiópatasejercicio físicofrecuencia cardíaca de entrenamientocasos clínicos
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