Search results for " Farm"

DNA binding and biological activity of Cu(II) and Zn(II) complexes of a 2,5-diphenyl[1,3,4]oxadiazole macrocycle ligand.

2011

Apollon gene silencing induces apoptosis in breast cancer cells via p53 stabilisation and caspase-3 activation

2009

We analysed the effects of small interfering RNA (siRNA)-mediated silencing of Apollon, a member of the inhibitors of apoptosis protein family, on the proliferative potential and ability of human breast cancer cell lines to undergo apoptosis. In wild-type p53 ZR75.1 cells, Apollon knockdown resulted in a marked, time-dependent decline of cell growth and an increased rate of apoptosis, which was associated with p53 stabilisation and activation of the mitochondrial-dependent apoptotic pathway. Pre-incubation of cells with a p53-specific siRNA resulted in a partial rescue of cell growth inhibition, as well as in a marked reduction of the apoptotic response, indicating p53 as a major player in …

SMOOTHLY SHIFTING FLUORESCENT WINDOW: TUNABLE “OFF-ON-OFF”MICELLAR BIOSENSORS FOR pH

2009

Motor Transitions' Peculiarity of Heterozygous DAT Rats When Offspring of an Unconventional KOxWT Mating.

2020

Abstract Causal factors of psychiatric diseases are unclear, due to gene × environment interactions. Evaluation of consequences, after a dopamine-transporter (DAT) gene knock-out (DAT-KO), has enhanced our understanding into the pathological dynamics of several brain disorders, such as Attention-Deficit/Hyperactivity and Bipolar-Affective disorders. Recently, our attention has shifted to DAT hypo-functional (heterozygous, HET) rodents: HET dams display less maternal care and HET females display marked hypo-locomotion if cared by HET dams (Mariano et al., 2019). We assessed phenotypes of male DAT-heterozygous rats as a function of their parents: we compared “maternal” origin (MAT-HET, obtain…

Demanda asistencial y análisis del coste farmacológico de la patología dermatológica en un centro de salud

2014

Aproximadamente del 15 al 20 % de las consultas médicas son por procesos cutáneos, y más del 85 % de las prescripciones de especialidades dermatológicas están realizadas por médicos no dermatólogos. Entre el 10 y el 20 % de los pacientes que acuden a la consulta del médico de familia presentan algún tipo de patología en la piel, y esto es reflejo de su alta prevalencia en la comunidad. La mayoría de las enfermedades de la piel diagnosticadas por los médicos de atención primaria son de etiología infecciosa o inflamatoria. Además existe una creciente demanda de visitas y tratamientos por motivos estéticos (acrocordones, angiomas capilares, quistes miliares ...) que pueden limitar el acceso a …

Environmental Enrichment During Adolescence Mitigates Cognitive Deficits and Alcohol Vulnerability due to Continuous and Intermittent Perinatal Alcoh…

2020

Perinatal alcohol exposure affects ontogenic neurodevelopment, causing physical and functional long-term abnormalities with limited treatment options. This study investigated long-term consequences of continuous and intermittent maternal alcohol drinking on behavioral readouts of cognitive function and alcohol vulnerability in the offspring. The effects of environmental enrichment (EE) during adolescence were also evaluated. Female rats underwent continuous alcohol drinking (CAD)—or intermittent alcohol drinking paradigm (IAD), along pregestation, gestation, and lactation periods—equivalent to the whole gestational period in humans. Male offspring were reared in standard conditions or EE un…

EMERGING ROLE OF ULTRASOUND IMAGING ASSOCIATED TO CLINICAL NEUROPHYSIOLOGY AS A SUPPLEMENTARY DIAGNOSTICS TECHNIQUE OF PERIPHERAL NERVES PATHOLOGIES:…

2013

PURPOSE: to show the relevance of peripheral nerve ultrasound imaging as a supplementary technique in a clinical neurophysiological evaluation. METHODS: peripheral nerves ultrasound (US) examination was performed in patients with a medical history of nerve entrapment and/or post-traumatic neuropathy but with clinical or neurophysiological unusual findings. A 10-18 MHz transducer was used. RESULTS: we recruited 50 patients with clinical evidence of peripheral neuropathies (25M, 25F): 24 median nerves, 1 anterior interosseous, 14 ulnar, 3 brachial plexus, 2 posterior interosseus, 1 superficial radial, 1 superficial peroneal and 4 common peroneal nerves. Overall 20/50 patients had a history of…

Buccal Thin Films as Potent Permeation Enhancers for Cytisine Transbuccal Delivery

2022

Cytisine (CYT) is a powerful anti-smoking compound which could greatly benefit from transbuccal delivery because of both its unfavorable pharmacokinetics after oral administration and its intrinsic ability to permeate the buccal mucosa. This work aims to design CYT-loaded buccal thin films suitable for transbuccal drug delivery due to its capability of promoting the interaction between CYT and the buccal membrane. The solvent casting method was employed to prepare several thin films combining various excipients such as matrixing polymers, mucoadhesion agents, plasticizers and other compounds as humectants and sweeteners, component ratios and solvents. A total of 36 compositions was prepared…

A robust reprogramming strategy for generating hepatocyte-like cells usable in pharmaco-toxicological studies.

2023

Abstract Background High-throughput pharmaco-toxicological testing frequently relies on the use of established liver-derived cell lines, such as HepG2 cells. However, these cells often display limited hepatic phenotype and features of neoplastic transformation that may bias the interpretation of the results. Alternate models based on primary cultures or differentiated pluripotent stem cells are costly to handle and difficult to implement in high-throughput screening platforms. Thus, cells without malignant traits, optimal differentiation pattern, producible in large and homogeneous amounts and with patient-specific phenotypes would be desirable. Methods We have designed and implemented a no…

THE ROLE OF THE CLINICAL PHARMACIST IN THE MANAGEMENT OF ANTIRETROVIRAL THERAPY

2020

Over the last few years, the Hospital Pharmacy has developed in an excellent way in Europe, proposing in many countries the clinical pharmacist as a new professional figure, and adapting its responsibilities to national health systems. The purpose of this study is to evaluate and promote the prescriptive appropriateness through the close collaboration between the pharmacist and the medical team. The aspect of the dispensation of antiretroviral therapy was deepened by providing useful information to the patient on the possible undesired effects and interactions due to the polypharmacy, on the correct way of storing the drugs, and on the importance of the regularity of the polypharmacy intake…