Search results for " Fertilization"

Biomass of spring barley (Hordeum vulgare L.) in relation to fertilising

2016

EFFECTS OF DIFFERENT FERTILIZATION LEVELS ON IBERIS SEMPERFLORENS POT CULTURE

2013

The genus Iberis (family Brassicaceae) includes 30 species of annual and perennial herbs and subshrubs that grow in a wide area from western and central Europe to the Mediterranean Basin. IberissemperflorensL. is a perennial evergreen subshrub (30-60 cm high) endemic to southern Italy and Sicily. Due to its abundance of white flowers and extensive flowering period occurring during the winter months, the species has good potential for pot cultivation and outdoor ornamental use in Mediterranean-type climates. Reliable and efficient propagation methods for I. semperflorens nursery production have been determined. However, there are limited information on cultural techniques of this winter flow…

Evidence for an intra- and extraplastidic pre-chorismate pathway.

1982

Pea plants grown under different conditions of cultivation, and eight different plant species with variegated leaves were used to study the intracellular localization of shikimate oxidoreductase (EC 1.1.1.25), the marker enzyme of the pre-chorismate pathway. The two series of experiments indicated an intra-and an extraplastidic compartimentalization of the enzyme, and both enzyme activities are regulated differentially. While the extraplastidic activity is permanently demonstrable, the intraplastidic activity is subject to the plants' developmental state and also depends on both illumination and fertilization.

Cuantificación de la calidad embrionaria mediante el consumo de oxígeno durante el proceso rutinario de incubación y desarrollo in vitro

2015

A pesar de la aplicación intensiva de las TRA y de las cada vez mayores tasas de éxito, hay ciertos aspectos de estos tratamientos que continúan sin resolverse, siendo una limitación importante el hecho de que sólo entre el 10-20% de los embriones transferidos al útero sean capaces de dar lugar a un embarazo a término. Esta limitación está promoviendo el desarrollo de nuevas técnicas no invasivas para seleccionar embriones más eficazmente que mediante el mero criterio morfológico. Las evidencias apoyadas por distintos trabajos encontrados en la literatura hacen que consideremos algunos métodos no invasivos, como la actividad metabólica de ovocitos y embriones, como buenos predictores de la …

Dynamique de la communauté microbienne de sols tropicaux suite à une application répétée de fertilisants organiques et minéraux

2019

Organic waste products (OWP) with NPK mineral fertilization are known to have an impact on soil microbial communities. However, dynamic of these communities following a repeated OWP and NPK application has not been well described. In the present study, this dynamic was observed on soil sampled at the La Mare (La Réunion) field experiment, which was set up in 2013. This experiment includes control plot (without organic input, but mineral fertilization) and several plots receiving different types of OWP coupled with NPK fertilization. Physico-chemical parameters were measured on soils and OWPs (2013 to 2017). Soil microbial biomass (from 2013 to 2018) and the structure of microbial community …

The Trumorph℗® system: The new univ the morphology of living sperm

2015

Abstract Evaluation of sperm morphology is a fundamental component of semen analysis, but its real significance has been obscured by a plethora of techniques that involve fixation and staining procedures that induce artefacts. Here we describe Trumorph℗ ® , a new method for sperm morphology assesment that is based upon examination of wet preparations of living spermatozoa immobilized by a short 60 °C shock using negative phase contrast microscopy. We have observed samples from five animals of the following species: bull, boar, goat and rabbit. In every case, all the components of the sperm head and tail were perfectly defined, including the acrosome and midpiece (in all its length, includin…

APOPTOTIC ANALYSIS OF CUMULUS CELLS FOR THE SELECTION OF COMPETENT OOCYTES TO BE FERTILIZED BY INTRACYTOPLASMIC SPERM INJECTION (ICSI)

2015

Oocyte quality is one of the main factors for the success of in vitro fertilization protocols. Apoptosis is known to affect oocyte quality and may impair subsequent embryonic development and implantation. The aim of this study was to investigate the apoptosis rate of single and pooled cumulus cells of cumulus cell–oocyte complexes (COCs), as markers of oocyte quality, prior to intracytoplasmatic sperm injection (ICSI).We investigated the apoptosis rate by TUNEL assay (DNA fragmentation) and caspase-3 immunoassay of single and pooled cumulus cells of COCs. The results showed that DNA fragmentation in cumulus cells was remarkably lower in patients who achieved a pregnancy than in those who di…

Impact on ICSI outcomes of adding 24 h of in vitro culture before testicular sperm freezing: a retrospective study.

2015

To compare sperm parameters and intracytoplasmic sperm injection (ICSI) outcomes for testicular spermatozoa frozen on the day of the biopsy (DO) with those frozen after 24 h of in vitro culture (D1).In this retrospective study, from 1999 to 2012, forty-nine azoospermic patients were included to compare sperm (motility and viability) and outcomes (fertilization (FR), implantation (IR), pregnancy (PR) and delivery rates (DR)).The in vitro culture increased total motility (+2.8 %, p = 0.0161) but decreased viability (-8.3 %, p = 0.007). After 24 h of culture, the post-thaw changes in motility and viability were not significant. Twenty-six couples underwent ICSI: thirty-four ICSI were performed…

Human Oviductal Gonadotropin-Releasing Hormone: Possible Implications in Fertilization, Early Embryonic Development, and Implantation1

2000

The oviduct is host to gametes and early embryos at a critical point in their lives. It is clear that the interactions of gametes/early embryo with the maternal oviduct in an autocrine and paracrine manner provide a microenvironment that enhances fertilization, early embryonic development, and implantation. Moreover, there is considerable evidence that an extrahypothalamic GnRH may play a substantial role as a molecular autocrine/paracrine regulator in these events. Gametes and preimplantation embryos express GnRH and GnRH receptor at both messenger ribonucleic acid (mRNA) and protein levels. However, whether GnRH is produced by the human oviduct has not yet been demonstrated. We used RT-PC…

SOME COMPARATIVE REMARKS ON THE TRANSIENT CHANGE IN LACTIC ACID CONTENT IN SEA URCHIN EGGS FOLLOWING FERTILIZATION.

1964

Abstract During the first few minutes following fertilization a transient increase in the concentration of lactic acid occurs in the eggs of Arbacia lixula , whereas no change at all is observed in the eggs of Paracentrotus liuidus . In the eggs of Psammechinus miliaris there is a transient decrease, soon followed by a recovery so that the level of the unfertilized egg is again reached.