Search results for " Fibroblast"
showing 10 items of 140 documents
Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crysta…
2022
Innovative engineering design for biologically active hydroxyapatites requires enhancing both mechanical and physical properties, along with biocompatibility, by doping with appropriate chemical elements. Herein, the purpose of this investigation was to evaluate and elucidate the model of naturally occurring hydroxyapatite and the effects of doped trace elements on the function of normal human fibroblasts, representing the main cells of connective tissues. The substrates applied (geological apatites with hexagonal prismatic crystal habit originated from Slyudyanka, Lake Baikal, Russia (GAp) and from Imilchil, The Atlas Mountains, Morocco (YAp)) were prepared from mineral natural apatite wit…
Comparative cytotoxicity evaluation of eight root canal sealers
2017
Background The aim of the present study is to evaluate and compare the cytotoxic effects of eight root canal sealers (BioRoot RCS, TotalFill BC Sealer, MTA Fillapex, Sealapex, AH Plus, EasySeal, Pulp Canal Sealer, N2) on immortalized human gingival fibroblasts over a period of 24, 48 and 72 hours. Material and Methods Immortalized human gingival fibroblast-1 HGF-1 (ATCC CRL-2014) were incubated. Root canal sealers were then placed into sterile, cylindrical Teflon moulds. The extraction was made eluting the sealers in cell culture medium. Cells (1 × 104) were seeded in each well of a 96-well plate and incubated for 24 h at 37°C. Cultures were then exposed to 100 μL of the extracts medium. Th…
The effect of light curing units and modes on cytotoxicity of resin-core systems
2010
Objective: The aim of this study was to compare the cytotoxic effects of various resin-based core materials that were cured with three light curing units (LCUs) in different modes on L?929 mouse fibroblast cells over 24 h and 72 h periods. Study design: Eighty-four cylindrical discs (2 mm in thickness and 6 mm in diameter) of each material (Rebilda, Voco; Build-It FR, Pentron; Clearfil DC Core, Kuraray and Bis-core, Bisco) were cured by QTH LCU (soft-up and high-power modes), LED LCU (exponential and standard modes) and PAC LCU (normal and ramp-curing modes). Then the samples were aged for 24 and 72 hours in Dulbecco?s Modified Eagle Medium/Ham?s F12 (DMEM/F12). After each ageing interval, …
The cytotoxicity of resin composites cured with three light curing units at different curing distances.
2011
Objective: The purpose of this study was to compare the effect of light curing distance on the cytotoxicity of five resin composites cured with three high-power light curing units. Study design: Seven cylindrical discs of each material (Grandio ®, Voco; Filtek ? Z250, 3M ESPE; Clearfil ? AP-X, Kuraray Co. Ltd.; Aelite ? LS, Bisco Inc. and Simile ®, Pentron) were cured. For curing, soft-up mode of quartz-tungsten-halogen, exponential mode of light emitting diode for 20 s, and ramp-curing mode of plasma arc light curing units for 6 s were used. The curing tip distances were determined as 2 and 9 mm and controlled via the use of metal rings. After ageing the samples for 24 and 72 hours in Dulb…
Aneuploid IMR90 cells induced by depletion of pRB, DNMT1 and MAD2 show a common gene expression signature
2019
Chromosome segregation defects lead to aneuploidy which is a major feature of solid tumors. How diploid cells face chromosome mis-segregation and how aneuploidy is tolerated in tumor cells are not completely defined yet. Thus, an important goal of cancer genetics is to identify gene networks that underlie aneuploidy and are involved in its tolerance. To this aim, we induced aneuploidy in IMR90 human primary cells by depleting pRB, DNMT1 and MAD2 and analyzed their gene expression profiles by microarray analysis. Bioinformatic analysis revealed a common gene expression profile of IMR90 cells that became aneuploid. Gene Set Enrichment Analysis (GSEA) also revealed gene-sets/pathways that are …
Mistranslation Drives Alterations in Protein Levels and the Effects of a Synonymous Variant at the Fibroblast Growth Factor 21 Locus.
2021
This article also appears in: Health, Medical, and Life Sciences Virtual Issue for Advanced Science.
The Co‐mutational Spectrum Determines the Therapeutic Response in Murine FGFR2 Fusion‐Driven Cholangiocarcinoma
2021
Background and aims Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer and a highly lethal malignancy. Chemotherapeutic options are limited, but a considerable subset of patients harbors genetic lesions for which targeted agents exist. Fibroblast growth factor receptor 2 (FGFR2) fusions belong to the most frequent and therapeutically relevant alterations in ICC, and the first FGFR inhibitor was recently approved for the treatment of patients with progressed, fusion-positive ICC. Response rates of up to 35% indicate that FGFR-targeted therapies are beneficial in many but not all patients. Thus far, no established biomarkers exist that predict resistance or r…
A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology
2015
Bone morphogenetic proteins (BMPs), members of the TGF-β superfamily, have numerous biological activities including control of growth, differentiation, and vascular development. Using an in vitro co-culture endothelial cord formation assay, we investigated the role of a BMP7 variant (BMP7v) in VEGF, bFGF, and tumor-driven angiogenesis. BMP7v treatment led to disruption of neo-endothelial cord formation and regression of existing VEGF and bFGF cords in vitro. Using a series of tumor cell models capable of driving angiogenesis in vitro, BMP7v treatment completely blocked cord formation. Pre-treatment of endothelial cells with BMP7v significantly reduced their cord forming ability, indicating …
Effects of mechanical stimulation on cell cycle duration in rat gingival fibroblast progenitor cells
2001
The aim of this investigation was to estimate cell cycle duration in rat gingival fibroblast progenitor cells in steady-state control and during sustained mechanical stimulation. Elastics (0.15 mm thick) were inserted between maxillary M1 and M2 of 8 wk-old male rats which were labelled with H3-TdR and killed in groups of 6-7 animals together with equal-sized groups of labelled control animals at intervals between 1-168 h. Autoradiographs of consecutive mesio-distal sections were used to determine grain counts for H3-TdR-labelled cells in the connective tissue of the gingival papilla between M2 and M3. Median cell cycle times (MCC) were estimated from plots of mean and median grain counts a…
A novel mutation in FGFR-3 disrupts a putative N-glycosylation site and results in hypochondroplasia
2000
Winterpacht, Andreas, Katja Hilbert, Christiane Stelzer, Thorsten Schweikardt, Heinz Decker, Hugo Segerer, Jürgen Spranger, and Bernhard Zabel. A novel mutation in FGFR-3 disrupts a putative N-glycosylation site and results in hypochondroplasia. Physiol. Genomics 2: 9–12, 2000.—Fibroblast growth factor receptor 3 (FGFR3) is a glycoprotein that belongs to the family of tyrosine kinase receptors. Specific mutations in the FGFR3 gene are associated with autosomal dominant human skeletal disorders such as hypochondroplasia, achondroplasia, and thanatophoric dysplasia. Hypochondroplasia (HCH), the mildest form of this group of short-limbed dwarfism disorders, results in ∼60% of cases from a mut…