Search results for " Intergenic"

showing 3 items of 63 documents

Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish.

2020

ABSTRACT Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about PGCs after their migration. We studied this post-migratory stage using microscopy and sequencing techniques, and found that many PGC-specific genes, including genes known to induce PGC fate in the mouse, are only activated several days after migration. At this same time point, PGC nuclei become extremely gyrated, displaying general broad opening of chromatin and high levels of intergenic transcription. This is accompanied by changes in nuage morphology, expression of large loci (PGC-expressed non-coding RNA loci, PERLs) that ar…

endocrine systemRNA UntranslatedTranscription GeneticZygotePiwi-interacting RNApiRNABiology03 medical and health sciences0302 clinical medicineGyrationTranscription (biology)Primordial germ cellmedicineAnimalsRNA Small InterferingMolecular BiologyZebrafishGeneZebrafish030304 developmental biologyCell NucleusNuage0303 health sciencesGonadal ridgeurogenital systemNuclear morphologyGene Expression Regulation DevelopmentalDNA-Directed RNA PolymerasesZygotic activationZebrafish Proteinsbiology.organism_classificationChromatinCell biologyUp-Regulationmedicine.anatomical_structureGerm CellsGenetic Loci207FertilizationMutationIntergenic transcriptionDNA Transposable ElementsDNA Intergenic030217 neurology & neurosurgeryGerm cellBiogenesisDevelopmental BiologyResearch ArticleDevelopment (Cambridge, England)
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Treated waste water reuse impact on soil microbial community structure as assessed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) fingerpr…

2007

International audience

microbe[SDV] Life Sciences [q-bio][SDE] Environmental Sciences[SDV]Life Sciences [q-bio]Automated Ribosomal Intergenic Spacer AnalysisARISA[SDE]Environmental Scienceswaste water reusefingerprint techniqueComputingMilieux_MISCELLANEOUSsoil
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Genetic relationship between clinical and environmental Vibrio cholerae isolates in Tanzania: A comparison using repetitive extragenic palindromic (R…

2015

The bacterium causing cholera, Vibrio cholerae, is a marine organism and coastal waters are important reservoirs of the organism. There are more than 200 serogroups of V. cholerae, of which serogroups O1 and O139 are known to be the causative agent of the cholera. The main virulent factor in V. cholerae is cholera toxin gene (ctx) that is found from the epidemic O1 and O139 strains, but may also be found in some strains other than O1 and O139 (non-O1 and non-O139). In this study, 48 V. cholerae strains isolated from three estuaries of Tanzania and 20 stool isolates were characterized in terms of their serogroups and possession of ctx gene and then compared using two PCR based fingerprinting…

ta1172VirulencePlant Sciencemedicine.disease_causeMicrobiologyMicrobiologyIntergenic regionestuaries of TanzaniamedicineGeneticsbiologyenterobacterial repetitive intergenic consensus (ERIC)-PCRGenetic heterogeneityCholera toxinta1183Outbreakta3142vibrio choleraebiology.organism_classificationmedicine.diseasebacterial infections and mycosesCholeraInfectious Diseasesrepetitive extragenic palindromic (REP)-PCRVibrio choleraeBacteria
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