Search results for " Isola"
showing 10 items of 482 documents
Isolation of Central Nervous System (CNS) Infiltrating Cells
2014
Leukocyte infiltration of the central nervous system (CNS) occurs under certain pathogenic conditions and most often results in severe disorders. Therefore, the isolation and analysis of such infiltrating cell populations is necessary for elucidating the underlying pathogenic mechanisms. Here we describe a simple and straightforward protocol for cell isolation from the inflamed CNS, which combines mechanical dissociation and enzymatic degradation of the tissue. Additionally, purification by Percoll gradient centrifugation provides a great yield of the infiltrating material. The isolated cells can be further used for downstream applications such as cell sorting, cellular or molecular analysi…
Analysis and review of the perioperative management of COVID-19 patients in thoracic surgery.
2021
Resumen Debido a la grave situacion de pandemia que vivimos causada por el COVID-19, la Seccion de Anestesia Cardiaca, Vascular y Toracica de la SEDAR pretende dar informacion de los aspectos mas relevantes a considerar en pacientes con sospecha o infeccion confirmada por coronavirus que van a ser sometidos a cirugia toracica. Creemos que es esencial mantener una optima transmision de informacion para llevar a cabo maniobras seguras, tanto para los pacientes como para el personal sanitario. La estrategia de manejo debe basarse en una organizacion exquisita del trabajo, definiendo quien, como y cuando va a realizar cada tarea y procedimiento. Las recomendaciones que se proponen, tras valorar…
Treatment choices and neuropsychological symptoms of a large cohort of early MS
2018
ObjectiveTo assess clinical characteristics, distribution of disease-modifying treatments (DMTs), and neuropsychological symptoms in a large cohort of patients with early-stage MS.MethodsThe German National MS Cohort is a multicenter prospective longitudinal cohort study that has recruited DMT-naive patients with clinically isolated syndrome (CIS) and relapsing-remitting MS (RRMS) since 2010. We evaluated their baseline characteristics and the prevalence of neuropsychological symptoms.ResultsOf 1,124 patients, with a 2.2:1 female-to-male ratio and median age at onset of 31.71 years (interquartile range [IQR]: 26.06–40.33), 44.6% and 55.3% had CIS and RRMS, respectively. The median Expanded …
"It's like being stuck on an unsafe and unpredictable rollercoaster": Experiencing substance use problems in a partner.
2020
Living with a partner with substance abuse problems may induce strains in an individual’s everyday life, including poor health, disrupted family life, and social isolation; this may lead to dropping out of education or work, a lack of safety and support, and facing various dilemmas and stigma.Aim:The purpose of this study was to explore these partners’ everyday life experiences, including their parental roles.Method:A qualitative design comprising qualitative interviews with ten partners and ex-partners was performed, and a thematic analysis was used.Results:The findings demonstrated that sharing their lives, including parenthood, with a partner with substance use problems affected every as…
„Eine Medizin der menschlichen Beziehungen” revisited - Aktuelle neurobiologische und psychosomatische Entwicklungen
2007
In his programmatic paper published 1989 in this journal, Herbert Weiner held the view that psychosomatic medicine has become a medicine of human relations. The aim of this review is TO reflect if current scientific evidence supports his view. Evidence from animal studies suggests that early infant-mother separation or neglect not only has a lasting influence on attachment behaviour, but also on brain development and the ability for stress regulation mediated by environmental as well as genetic factors. Neuronal networks processing affective components of physical pain play a role in signalling painful emotional experiences due to social isolation and separation. A consistent finding across…
The costs of friendship: severe mental illness, poverty and social isolation
2016
Background: The relationship between severe mental illness, poverty and social isolation has been explored in a number of studies.Aim: The purpose of the study was to explore the relationship betwe ...
Disconnected Lives: Trends in Time Spent Alone in Finland
2020
AbstractDiscussions about social isolation have been extensive over the past few decades. A less sociable nature of social ties has been identified in Western societies. The phenomenon has been associated with demographic changes such as aging and living alone as well as changes in the use of new technologies. In this study we employ representative Finnish Time Use Surveys from three decades, 1987–1988 (n = 1887), 1999–2000 (n = 2673) and 2009–2010 (n = 1887) to examine the trends in social isolation, measured as time spent alone. Our results showed that between 1987 and 2010 the time spent alone increased by 124 min per day. The increase was linear and occurred in nearly all population gro…