Search results for " LAB"
showing 10 items of 2393 documents
Cellular effects of bacterial N-3-Oxo-dodecanoyl-L-Homoserine lactone on the sponge Suberites domuncula (Olivi, 1792): insights into an intimate inte…
2014
International audience; Sponges and bacteria have lived together in complex consortia for 700 million years. As filter feeders, sponges prey on bacteria. Nevertheless, some bacteria are associated with sponges in symbiotic relationships. To enable this association, sponges and bacteria are likely to have developed molecular communication systems. These may include molecules such as N-acyl-L-homoserine lactones, produced by Gram-negative bacteria also within sponges. In this study, we examined the role of N-3-oxododecanoyl-L-homoserine lactone (3-oxo-C12-HSL) on the expression of immune and apoptotic genes of the host sponge Suberites domuncula. This molecule seemed to inhibit the sponge inn…
Identification of Trans-Golgi Network Proteins in Arabidopsis thaliana Root Tissue
2014
Knowledge of protein subcellular localization assists in the elucidation of protein function and understanding of different biological mechanisms that occur at discrete subcellular niches. Organelle-centric proteomics enables localization of thousands of proteins simultaneously. Although such techniques have successfully allowed organelle protein catalogues to be achieved, they rely on the purification or significant enrichment of the organelle of interest, which is not achievable for many organelles. Incomplete separation of organelles leads to false discoveries, with erroneous assignments. Proteomics methods that measure the distribution patterns of specific organelle markers along densit…
Biomedical applications of ion mobility-enhanced data-independent acquisition-based label-free quantitative proteomics.
2014
Mass spectrometry-based proteomics greatly benefited from recent improvements in instrument performance and the development of bioinformatics solutions facilitating the high-throughput quantification of proteins in complex biological samples. In addition to quantification approaches using stable isotope labeling, label-free quantification has emerged as the method of choice for many laboratories. Over the last years, data-independent acquisition approaches have gained increasing popularity. The integration of ion mobility separation into commercial instruments enabled researchers to achieve deep proteome coverage from limiting sample amounts. Additionally, ion mobility provides a new dimens…
Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
2012
The study of host-parasite interactions has increased considerably in the last decades, with many studies focusing on the identification of parasite molecules (i.e. surface or excretory/secretory proteins (ESP)) as potential targets for new specific treatments and/or diagnostic tools. In parallel, in the last few years there have been significant advances in the field of extracellular vesicles research. Among these vesicles, exosomes of endocytic origin, with a characteristic size ranging from 30-100 nm, carry several atypical secreted proteins in different organisms, including parasitic protozoa. Here, we present experimental evidence for the existence of exosome-like vesicles in parasitic…
General Statistical Framework for Quantitative Proteomics by Stable Isotope Labeling
2014
Pedro J. Navarro et al.
CiliaCarta: An integrated and validated compendium of ciliary genes
2019
The cilium is an essential organelle at the surface of mammalian cells whose dysfunction causes a wide range of genetic diseases collectively called ciliopathies. The current rate at which new ciliopathy genes are identified suggests that many ciliary components remain undiscovered. We generated and rigorously analyzed genomic, proteomic, transcriptomic and evolutionary data and systematically integrated these using Bayesian statistics into a predictive score for ciliary function. This resulted in 285 candidate ciliary genes. We generated independent experimental evidence of ciliary associations for 24 out of 36 analyzed candidate proteins using multiple cell and animal model systems (mouse…
The substrate degradome of meprin metalloproteases reveals an unexpected proteolytic link between meprin β and ADAM10
2012
The in vivo roles of meprin metalloproteases in pathophysiological conditions remain elusive. Substrates define protease roles. Therefore, to identify natural substrates for human meprin α and β we employed TAILS (terminal amine isotopic labeling of substrates), a proteomics approach that enriches for N-terminal peptides of proteins and cleavage fragments. Of the 151 new extracellular substrates we identified, it was notable that ADAM10 (a disintegrin and metalloprotease domain-containing protein 10)—the constitutive α-secretase—is activated by meprin β through cleavage of the propeptide. To validate this cleavage event, we expressed recombinant proADAM10 and after preincubation with meprin…
Teaching mechanical oscillations using an integrate curriculum
1997
Abstract A set of teaching materials dealing with harmonic oscillations was developed as part of a research project investigating the influence of different pedagogical tools on physics learning. The materials incorporate simulation software and laboratory activities associated with Teacher and Student Units. The project involved research groups at seven Italian universities and the materials have been trialled in high schools nation‐wide. This paper describes the experimental protocol for the use of these materials in pilot classes and the evaluation of student learning and teacher training results. The intervention was found to improve physics education at school praxis level and to promo…
Measurement of the Charge-Averaged Elastic Lepton-Proton Scattering Cross Section by the OLYMPUS Experiment
2020
Physical review letters 126(16), 162501 (1-6) (2021). doi:10.1103/PhysRevLett.126.162501
Pràctiques de recursos humans, clima laboral i resultats de gestió. Anàlisi del cas "Verdifresh"
2012
La crisi econòmica mundial obliga cada empresa a lliurar una batalla, a vegades amb si mateixa, per incrementar la seua competitivitat. Cada vegada més, les organitzacions són conscients de la necessitat de comptar amb treballadors alineats amb la seua estratègia. Per aconseguir-ho, algunes empreses s'esforcen a humanitzar les relacions laborals i redissenyar les seues pràctiques de gestió de recursos humans (RH). En aquest estudi s'analitza el cas de l'empresa Verdifresh. Les seues pràctiques de RH s'han dissenyat amb l'objectiu de fidelitzar els treballadors i millorar la seua percepció de les condicions laborals. En aquest treball s'analitzen aquest tipus de relacions i es descriuen les …