Search results for " LAB"
showing 10 items of 2393 documents
A new model of chronic colitis in SCID mice induced by adoptive transfer of CD62L+ CD4+ T cells: insights into the regulatory role of interleukin-6 o…
2003
<i>Objective:</i> The proinflammatory cytokine interleukin (IL)-6 is involved in various chronic inflammatory processes. IL-6 is a predominant cytokine produced by lamina propria T cells in Crohn’s disease and experimental colitis. This study was designed to examine the effect of a neutralizing IL-6-receptor (IL-6R) antibody on the programmed cell death of mucosal T cells in the CD62L+ CD4+ SCID transfer model of chronic experimental colitis in mice and to gain more insight into the pathogenesis of this transfer colitis model. <i>Methods:</i> For adoptive transfer, we isolated CD62L+ CD4+ double-positive T cells from wild-type BALB/c mice followed by intraperitoneal …
Optimized Protocol for the Detection of Multifunctional Epitope-Specific CD4+ T Cells Combining MHC-II Tetramer and Intracellular Cytokine Staining T…
2019
Analysis of multifunctional CD4+ T cells is fundamental for characterizing the immune responses to vaccination or infection. Major histocompatibility complex (MHC)/peptide tetramers represent a powerful technology for the detection of antigen-specific T cells by specific binding to their T-cell receptor, and their combination with functional assays is fundamental for characterizing the antigen-specific immune response. Here we optimized a protocol for the detection of multiple intracellular cytokines within epitope-specific CD4+ T cells identified by the MHC class II tetramer technology. The optimal procedure for assessing the functional activity of tetramer-binding CD4+ T cells was based o…
Transverse-momentum-dependent Multiplicities of Charged Hadrons in Muon-Deuteron Deep Inelastic Scattering
2017
A semi-inclusive measurement of charged hadron multiplicities in deep inelastic muon scattering off an isoscalar target was performed using data collected by the COMPASS Collaboration at CERN. The following kinematic domain is covered by the data: photon virtuality $Q^{2}>1$ (GeV/$c$)$^2$, invariant mass of the hadronic system $W > 5$ GeV/$c^2$, Bjorken scaling variable in the range $0.003 < x < 0.4$, fraction of the virtual photon energy carried by the hadron in the range $0.2 < z < 0.8$, square of the hadron transverse momentum with respect to the virtual photon direction in the range 0.02 (GeV/$c)^2 < P_{\rm{hT}}^{2} < 3$ (GeV/$c$)$^2$. The multiplicities are pres…
The Comprehensive Economic and Trade Agreement (CETA): What Kind of Space is Given to Fair Trade and Labour Rights?
2017
Il presente lavoro analizza da una prospettiva giuslavoristica il Comprehensive Economic and Trade Agreement (CETA), trattato internazionale di libero scambio, da poco sottoscritto da Unione Europea e Canada: il saggio si interroga se tale trattato possa contribuire o meno allo sviluppo del fair trade all'interno delle democrazie occidentali. Nel dettaglio l'articolo analizza le clausole del CETA alla luce dei core labour standards dell'ILO, con un focus sulle clausole arbitrali di risoluzione delle controversie (Investor-State Dispute Settlement), sulle forme di partecipazione della società civile e sui meccanismi di eccezione. Nelle conclusioni, nonostante la tendenziale omogeneità socio-…
A REVIEW OF COLOR MEASURMENTS IN THE TEXTILE INDUSTRY
2016
Color is an important factor in the evaluation of aesthetic appearance and functionality of many products, but especially of textile industry ones. In textiles production process, color can be assessed in different stages: the selection of raw materials, the incoming item tests, the preparation of dyeing ingredients, the crocking resistance testing, the color fastness and in all stages, the quality control. Color evaluation can be done visually or using specialized test instruments such as colorimeters or spectrometers, therefore a high accuracy of measurements must be achieved. Standards describe different procedures and testing techniques depending on the product type and the quality leve…
Pacientu pieredze COVID – 19 nodaļā un tās ietekme uz sociālo interakciju un psihoemocionālo labsajūtu pēc hospitalizācijas
2022
Maģistra darba tēma “Pacientu pieredze COVID – 19 nodaļā un tās ietekme uz sociālo interakciju un psihoemocionālo labsajūtu pēc hospitalizācijas”. Psihiskā veselība ir nozīmīga un neatņemama vispārējās veselības komponente. Laba indivīda un kopējā sabiedrības psihiskā veselība ir būtisks nosacījums stabilas, drošas un labklājīgas sabiedrības veidošanai. (10). Darba mērķis ir noskaidrot pacientu pieredzi COVID – 19 un tās ietekmi uz sociālo interakciju un psihoemocionālo labsajūtu pēc hospitalizācijas. Vadoties pēc izvirzītā mērķa, tā sasniegšanai maģistra darbā tika risināti šādi uzdevumi: izanalizēt un apkopot literatūras avotus par dzīves kvalitāti, par psihoemocionālo labsajūtu, sociālo …
Risk factors associated with adverse fetal outcomes in pregnancies affected by Coronavirus disease 2019 (COVID-19): a secondary analysis of the WAPM …
2020
Abstract Objectives To evaluate the strength of association between maternal and pregnancy characteristics and the risk of adverse perinatal outcomes in pregnancies with laboratory confirmed COVID-19. Methods Secondary analysis of a multinational, cohort study on all consecutive pregnant women with laboratory-confirmed COVID-19 from February 1, 2020 to April 30, 2020 from 73 centers from 22 different countries. A confirmed case of COVID-19 was defined as a positive result on real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay of nasal and pharyngeal swab specimens. The primary outcome was a composite adverse fetal outcome, defined as the presence of either abortion (pre…
Obstetric-Neonatal Care during Birth and Postpartum in Symptomatic and Asymptomatic Women Infected with SARS-CoV-2: A Retrospective Multicenter Study
2022
Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/1660-4601/19/9/5482 Este artículo de investigación pertenece a la colección "COVID-19 Research". En este artículo de investigación también participan: Laura Andreu-Pejó, Cristina Martínez-Porcar, Carmen Rodríguez Gonzálvez, Patricia Torrent-Ramos, Nieves Asensio-Tomás, Yolanda Herraiz-Soler, Ramon Escuriet y Desirée Mena-Tudela. This study analyses the obstetric–neonatal outcomes of women in labour with symptomatic and asymptomatic COVID-19. A retrospective, multicenter, observational study was carried out between 1 March 2020 and 28 February 2021 in eight public hospitals in the Valencian community (Spain). The…
The virtuous cycle of trust. Unveiling clues to successful innovation in the Fast Moving Consumer Goods industry
2022
PurposeThis paper aims to shed light on features of successful innovation and on the role played by downstream open innovation (OI) dynamics in the Fast Moving Consumer Goods (FMCG) industry. It also explores the reasons for the decline in the number of disruptive innovations in this industry in the European Union (EU).Design/methodology/approachThe authors interpret the data in a Kantar consumer panel dataset on disruptive innovations in Spain through an exploratory research including in-depth interviews with 19 managers in the industry.FindingsResults show that downstream open innovation in this industry is mostly limited to the executional stage in the process and highlight the crucial r…
Immunogold Labeling to Detect Streptococcus pyogenes Cas9 in Cell Culture and Tissues by Electron Microscopy
2019
Final publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/crispr.2019.0032. The CRISPR-Cas9 system is a powerful and yet precise DNA-editing tool in rapid development. By combining immunogold labeling and electron microscopy with the novel CRISPR-Cas9 system, we propose a new method to gain insight into the biology of this tool. In this study, we analyzed different Cas9-induced systems such as HEK293T cell line, murine oligodendrocyte progenitor cells, brain and liver to detect Cas9 expression by immunoelectron microscopy. Our results show that while Cas9 expression could be found in the nuclei and nucleopores of transfected HEK293T cells, in transfected…