Search results for " LAB"

Integrated annual calcium carbonate production of planktic calcifiers in the North Pacific from the R/V Kilo Moana cruise KM1712

2022

This dataset compiles the estimates of annual CaCO3 production, including the upper and lower limits of the estimates, for the 4 planktic calcifying groups considered in the study, the pteropods (mg/m²/yr), the heteropods (mg/m²/yr), the foraminifers (mg/m²/yr) and the coccolithophores (mg/m²/yr). The estimates derived from the living standing stocks of these 4 groups of organisms collected in the North Pacific between Hawaii and the Gulf of Alaska during the R/V Kilo Moana cruise KM1712 in August 2017. R code was used to calculate the integrated annual CaCO3 production for the different organisms, including the upper and lower limits (Gray, 2022).

Can Hepatoma Cell Lines be Re-differentiated to be Used in Drug Metabolism Studies?

2013

Knowledge of metabolism, enzymes so far involved, and potential enzyme-inhibiting or enzyme-inducing properties of new compounds is a key issue in drug development. Primary cultured hepatocytes, cytochrome P450 (CYP)-engineered cells and hepatoma cell lines are currently being used for this purpose, but only primary cultures can produce a metabolic profile of a drug similar to that found in vivo and can respond to inducers. Because of their limited accessibility, alternatives to replace human hepatocytes are currently being explored, including the immortalisation of hepatocytes by using different strategies (i.e. SV40 T-large antigen, conditionally immortalised hepatocytes, transfection wi…

Synergistic Effect of Carfilzomib and Metformin in Vascular Plasticity; The Emerging Role of Autophagy

2019

Introduction: Carfilzomib (Cfz) correlates with a risk of reversible cardiotoxicity in 5-10% of multiple myeloma (MM) patients. We have recently shown that metformin (Met) has a prophylactic role against the Cfz-induced cardiotoxicity in vivo, through activation of AMPKα signaling (Blood 2019;133:710-23). However, the impact of Cfz on vascular function is obscure. Therefore, we sought to investigate: i) the acute, ii) the sub-chronic effect of Cfz on the vascular reactivity, iii) the effect of metformin co-administration on the vascular phenotype and iv) the impact of Cfz and Met co-administration on aged Human Aortic Smooth Muscle Cells (HAoSMCs). Methods: Forty male C57Bl/6 mice were assi…

La Europa social / 4. La demolición

2005

La casa ipertestuale. Libertà e complessità nell'abitare

2008

L'articolo fa riferimento, in generale, all’esperienza del workshop "Futura, come abiteremo domani" che ha avuto luogo nei laboratori della Facoltà di Architettura di Palermo nel marzo del 2005. Iniziativa nata da una sollecitazione di Lucio Dalla ed in collaborazione con Expa, galleria di Architettura. Al workshop hanno partecipato studenti della facoltà provenienti dai corsi di laurea in Architettura 4/s e Disegno Industriale, e si è articolato in sei laboratori diretti dai professori Teresa La Rocca (coordinatore), Emanuele Palazzotto, Marcello Panzarella, Michele Sbacchi, Andrea Sciascia, Viviana Trapani. In particolare, il tema di progetto affrontato all’interno del Laboratorio del Pro…

Causalidad en salud laboral: el caso ardystil

1995

ResumenEl establecimiento de relaciones causa-efecto ha sido y sigue siendo objeto de debate en epidemiología. La naturaleza observacional de la investigación epidemiológica dificulta el reconocimiento de estas relaciones. En este contexto, se han propuesto diferentes modelos para explicar las relaciones causales en los procesos de salud y enfermedad, desde el modelo determinista puro defendido por los postulados de Koch, que requiere la aceptación de relaciones unicausales, pasando por otras explicaciones que asumen la naturaleza multicausal de los problemas de salud en la población. En Salud Laboral se debe disponer también de modelos explicativos y criterios consensuados para valorar las…

High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.

2021

Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines. The preliminary test for ESBL-producing Enterobacteriaceae was performed by replicating enterobacte…

Assessment of Escherichia coli B with enhanced permeability to fluorochromes for flow cytometric assays of bacterial cell function.

2002

Background Flow cytometry has become a choice methodology for microbiological research. However, functional cytometric assays in live bacteria are still limited. This is due, in part, to the cell wall impairing penetration of vital dyes in bacteria, thus imposing permeabilization procedures. These manipulations may affect cell physiology, provoke cell aggregation or lysis, and they are time-consuming. Escherichia coli B strains have been used for mutagenic assays because of an altered lipopolysaccharide that provokes increased membrane permeability. We assessed the use of these strains as possible alternatives for flow cytometric assays to avoid the permeabilization steps. Methods Suspensio…

The selection of serum-independent PC12 cells for a more-reliable manganese cytotoxicity test.

2007

A major issue concerning the protocols of heavy metal cytotoxicity tests with PC12 cells was the hypothesis that serum in the culture medium might sequester the metal, thus altering the results obtained. However, serum withdrawal impairs the viability of PC12 cells themselves, thus impeding cytotoxicity testing in the absence of serum. In this study, we repeatedly selected undifferentiated, totally non-adherent PC12 cells in Petri dishes. Surprisingly, we discovered that these cells could survive and proliferate in serum-free medium. Moreover, features such as NGF-responsiveness, resazurin reduction potential, doubling rate, protein content, and basal caspase-3 enzyme activity, were equiva…

Geographical mapping of metabolites in biological tissue with quantitative bioluminescence and single photon imaging

1993

This article features a novel technique for measuring the spatial distribution of metabolites, such as ATP, glucose, and lactate, in rapidly frozen tissue. Concentration values are obtained in absolute terms and with a spatial resolution of single-cell dimension. The method is based on enzymatic reactions that link the metabolite of interest to luciferase with subsequent light emission. Using a specific array, cryosections are brought into contact with the enzymes in a well-defined, reproducible way inducing a distribution of light across the section with an intensity that is proportional to the metabolite concentration. The emitted light can be visualized through a microscope and an imagin…