Search results for " LAB"
showing 10 items of 2393 documents
Inducible Genetic Code Expansion in Eukaryotes
2020
Abstract Genetic code expansion (GCE) is a versatile tool to site‐specifically incorporate a noncanonical amino acid (ncAA) into a protein, for example, to perform fluorescent labeling inside living cells. To this end, an orthogonal aminoacyl‐tRNA‐synthetase/tRNA (RS/tRNA) pair is used to insert the ncAA in response to an amber stop codon in the protein of interest. One of the drawbacks of this system is that, in order to achieve maximum efficiency, high levels of the orthogonal tRNA are required, and this could interfere with host cell functionality. To minimize the adverse effects on the host, we have developed an inducible GCE system that enables us to switch on tRNA or RS expression whe…
Spectroscopic, radiochemical, and theoretical studies of the Ga3+-N-2-hydroxyethyl piperazine-N'-2-ethanesulfonic acid (HEPES buffer) system: evidenc…
2013
Recent reports have claimed a superior performance of HEPES buffer in comparison to alternative buffer systems for 67/68 Ga labeling in aqueous media. In this paper we report spectroscopic (1H and 71 Ga NMR), radiochemical, mass spectrometry and theoretical modeling studies on the Ga3+/HEPES system (HEPES = N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid) performed with the aim of elucidating a potential contribution of HEPES in the 68/67 Ga radiolabeling process. Our results demonstrate that HEPES acts as a weakly but competitive chelator of Ga3+ and that this interaction depends on the relative Ga3+: HEPES concentration. A by-product formed in the labeling mixture has been identified …
Job strain in public transport drivers: Data to assess the relationship between demand-control model indicators, traffic accidents and sanctions
2018
This Data in Brief (DiB) article examines the association between the Job Demand-Control (JDC) model of stress and traffic safety outcomes (accidents and sanctions) in public transport drivers (n = 780). The data was collected using a structured self-administrable questionnaire composed of measurements of work stress (Job Content Questionnaire), and demographics (professional driving experience, hours and days working/driving per week). The data contains 4 parts: descriptive statistics, bivariate correlations between the study variables, analysis of variance (ANOVA) and Post-Hoc comparisons between drivers classified different quadrants of the JDC model. For further information, it is conve…
Addressing informality, gender and ethnicity in domestic labour
2014
The legal framework concerning household employment has undergone a number of changes worldwide. The 100th International Labour Conference in June 2011 was highly significant as it adopted the first international labour standards specifically for domestic workers: Convention No. 189 and the supplementing Recommendation No. 201. This article analyses recent Spanish reforms (2011–2013) in order to assess the effects of these changes, taking into account the characteristics of domestic work: feminized, informal, ethnicized and vulnerable. Although aimed at improving employment and working conditions, the new Spanish legislation has resulted in a number of setbacks as well as some progress. The…
Influence of Pendant Arms Bearing Ligating Groups on the Structure of Bismuth Porphyrins: Implications for Labeling Immunoglobulins Used in Medical A…
2004
International audience; The synthesis of two picket bismuth(III) porphyrins is reported, and their crystal structures are compared. The influence of the nature of the pickets, as well as their number, is discussed in terms of stability, kinetics of metalation, structure, and distortion of the porphyrin. Unexpectedly, the resultsindicate that the coordination sphere of bismuth is not affected by different types of distortion nor is the stability of the complex. For both types of complexes, the X-ray crystallography revealed that a single arm is actually in direct interaction with the bismuth cation, thereby allowing furtherfunctionalization on the porphyrin core as the linkage with immunoglo…
Panorama de la Calidad de Vida Laboral entre Trabajadores que Prestan Cuidados Paliativos en Brasil
2019
Resumo Este artigo tem por objetivo oferecer um primeiro diagnóstico sobre os níveis de qualidade de vida dos trabalhadores brasileiros que prestam cuidados paliativos. Foram aplicadas escalas de medidas de qualidade de vida profissional, en-frentamento ante a morte, autocuidado profissional e autoconsciência em 161 profissionais. As análises de dados incluíram estatísticas descritivas, comparação dos níveis em função de sexo, idade e outras características sociodemográficas, e estudo da relação entre as variáveis mencionadas. Foram encontrados níveis adequados tanto nas variáveis de proteção da qualidade de vida profissional como na qualidade de vida em si. As comparações em função de dado…
Bridging vs. chelating coordination modes of vinylsilanes in CuI π-Complexes: Structure and stability
2014
Two new copper(I) olefin complexes, [Cu6Cl6(MTrVS)2] (1) and [Cu2Cl2(DMVSP)2] (2), of tridentate bridging methyltrivinylsilane (MTrVS) and bidentate chelating 2-[dimethyl(vinyl)silyl]pyridine (DMVSP) have been synthesized and characterized by single-crystal X-ray structure analysis, IR and 1H NMR spectroscopy. It has been shown that using the alkenylsilanes with required electronic properties, molecular symmetry and conformational flexibility, it is possible to control the formation of optimal copper(I) halide oligomers. The obtained results, together with relevant literature data, also illustrate how the coordination mode of vinylsilanes is related to Cu–(C=C) bond strengthening and, conse…
Digital Labour, Pandemic COVID-19, and Emotions
2021
This chapter seeks to outline in a preliminary way the consequences of the pandemic in work in general and especially in digital work in its connection with emotions and the politics of sensibilities. Based on public and private reports and the opinion of experts, some traces are presented here that allow us to reconstruct some changes in the politics of sensibilities that have occurred due to the socio-labour pandemic impact. In this sense, the following argumentative strategy has been developed: 1) the theoretical perspective on the connection between digital and political work of sensibilities is outlined, 2) some of the main consequences of the pandemic at work are synthesized, and 3) b…
Morning and evening type: The cortisol awakening response in a sleep laboratory.
2019
Abstract Background Earlier evidence indicated an association between chronotype and the cortisol awakening response (CAR). However, these earlier data were collected without objective control for participant compliance being available, which, following recent guidelines, is considered critical for ensuring data validity. Here, we are seeking to replicate these earlier findings within a well-controlled sleep laboratory set up. Methods The sample consisted of 103 young males, including 12 participants defined as ‘evening’ (MEQ 59) types. All participants slept for one night in a sleep laboratory and were wakened at 6:30 AM the next morning. Saliva samples for the assessment of the CAR were…