Search results for " LAB"
showing 10 items of 2393 documents
The zoom-on-possessee construction in Kam (Dong): the anatomy of a new construction type
2005
Kam, a Kadai language spoken in Guizhou province (People's Republic of China), has a family of intransitive possessive constructions with the word order ‘Possessor–Verb–Possessee’. (The basic word order in Kam is SV and AVO.) While two recent papers have featured this unique construction type for an array of other Southeast Asian languages, they fail to acknowledge its distinct semantic value in contrast to the related construction type ‘Possessee–Possessor–Verb’. The former construction type displays a so-called ‘zoom-effect’: the possessor is predicated IN, AT or THROUGH his/her/its possessee; the predication zooms from the possessor on his/her/its possessee. The latter construction, in c…
Flora Ossette’s ‘feminist visibility’ in her translation of Olive Schreiner’s Woman and Labour
2011
La visibilidad del traductor o la traductora se ha convertido en tema obligado en los estudios de traducción desde que Lawrence Venuti editara en 1995 su conocido The Translator’s Invisibility. En la única traducción al castellano de Woman and Labour de Olive Schreiner por parte de Flora Ossette no solo es posible apreciar la voz de la “traductora implícita” (Hermans 1996), sino una voz “explícita”. La traductora interviene activamente en el texto que traduce añadiendo, omitiendo, reorganizando, apostillando o enfatizando las ideas de Schreiner. Además, llevada por los ideales feministas que comparten ambas y por su admiración por la autora sudafricana, se convierte en autora redactando un …
Análisis de la satisfacción laboral del profesorado de español como lengua extranjera (ELE)
2020
En este artículo se presentan los resultados de una investigación cuantitativa de carácter exploratorio sobre el grado de satisfacción del profesorado de ELE sobre sus condiciones laborales y perspectivas profesionales. Se discuten críticamente los datos recogidos mediante un cuestionario distribuido en línea entre 673 docentes, que mide la satisfacción en ocho dimensiones: organizativa, económica, afectiva, motivacional, discriminación, ambiente laboral, realización profesional y beneficios sociales. Los resultados permiten obtener una primera panorámica de las condiciones temporales, organizativas, económicas, sociales y psicológicas de la profesión. A pesar de algunas limitaciones relaci…
The use of cultured hepatocytes to investigate the metabolism of drugs and mechanisms of drug hepatotoxicity.
2001
Hepatotoxins can be classified as intrinsic when they exert their effects on all individuals in a dose-dependent manner, and as idiosyncratic when their effects are the consequence of an abnormal metabolism of the drug by susceptible individuals (metabolic idiosyncrasy) or of an immune-mediated injury to hepatocytes (allergic hepatitis). Some xenobiotics are electrophilic, and others are biotransformed by the liver into highly reactive metabolites that are usually more toxic than the parent compound. This activation process is the key to many hepatotoxic phenomena. Mitochondria are a frequent target of hepatotoxic drugs, and the alteration of their function has immediate effects on the ene…
Dual Labeling of Lipopolysaccharides for SPECT-CT Imaging and Fluorescence Microscopy.
2013
International audience; : Lipopolysaccharides (LPS) or endotoxins are amphipathic, pro-inflammatory components of the outer membrane of Gram-negative bacteria. In the host, LPS can trigger a systemic inflammatory response syndrome. To bring insight into in vivo tissue distribution and cellular uptake of LPS, dual labeling was performed with a bimodal molecular probe designed for fluorescence and nuclear imaging. LPS were labeled with DOTA-Bodipy-NCS, and pro-inflammatory properties were controlled after each labeling step. LPS were then radiolabeled with (111)In and subsequently injected intravenously into wild-type, C57B16 mice, and their in vivo behavior was followed by single photon emis…
Application of X-ray microanalysis to study of the expression of endothelial adhesion molecules on human umbilical vein endothelial cells in vitro
1994
A semi-quantitative procedure is described, which allows the evaluation of expression levels of endothelial adhesion molecules on cultured human umbilical vein endothelial cells (HUVEC) using energy dispersive X-ray microanalysis (EDX). As a model two adhesion molecules, E-selection (CD62E; ELAM-1/endothelial leukocyte adhesion molecule-1) and ICAM-1 (intercellular adhesion molecule-1; CD54), were localized by the use of the silver-enhancement colloidal gold method after stimulation of HUVEC with endotoxin lipopolysaccharide (LPS), tumour necrosis factor (TNF) or a phorbol ester (PMA). The analysis was performed in a scanning electron microscope (SEM) at an accelerating voltage of 15 kV wit…
Two-step impact of Amphotericin B (AmB) on lipid membranes: ESR experiment and computer simulations.
2013
In this study, the electron spin resonance (ESR) method was used to examine the effect of Amphotericin B (AmB) molecules on the fluidity of model membranes made of dipalmitoylphosphatidylcholine (DPPC). The changes occurring under increased AmB concentrations in the spectroscopic parameters of spin probes placed in liposomes were determined. Three probes were used, penetrating the membrane at different depths which allowed the changes in its fluidity to be found in the transverse section. A computer model of the surface layer of membrane, with AmB admixture, was developed and subjected to computer simulation. The effect of changing concentration of the admixture on the binding energy in the…
The impact of humic and fulvic acids on the dynamic properties of liposome membranes: the ESR method
2013
This paper presents the results of research on the influence of two fractions of humic substances (HS): fulvic acids (FA) and humic acids (HA), as a function of concentration, on the liposome membranes formed from egg yolk lecithin (EYL). The concentration of HS in relation to EYL changed from 0% to 10% by weight. The influence of HS on various areas of membranes: interphase water-lipid, in the lipid layer just below the polar part of the membrane and in the middle of the lipid bilayer, was investigated by different spin labels (TEMPO, DOXYL 5, DOXYL 16). The study showed that HA slightly decreased the fluidity of the analyzed membranes on the surface layer, while FA significantly liquidate…
Membrane fluidity and the surface properties of the lipid bilayer: ESR experiment and computer simulation
2009
Penetration of the liposome membranes formed in the gel phase from DPPC (DPPC liposomes) and in the liquid-crystalline phase from egg yolk lecithin (EYL liposomes) by the TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) and 16 DOXYL (2-ethyl-2-(15-methoxy-oxopentadecyl)-4,4-dimethyl-3-oxazolidinyloxy) spin probes has been investigated. The penetration process was followed by 120 hours at 24(0)C, using the electron spin resonance (ESR) method. The investigation of the kinetics of the TEMPO probe building into the membranes of both types of liposomes revealed differences appearing 30 minutes after the start of the experiment. The number of TEMPO particles built into the EYL liposome membranes beg…
Louis Jacques Thenard's Chemistry Courses at the Collège de France, 1804–1835
2010
This article is concerned with the public courses and lecture demonstrations given by Louis Jacques Thenard at the College de France during the first decades of the nineteenth century. The expectations and needs of Thenard's auditors will be studied in order to understand the role played by chemistry courses at the College in the context of the growing and changing Parisian teaching market during the first third of the nineteenth century. The preparation and performance of lecture demonstrations was the main driving force of several major changes in the premises and the personnel associated with the chair of chemistry. Our analysis of the parallel process of expansion and functional differe…