Search results for " Leaves"

Mercury uptake into poplar leaves

2016

International audience; Tailings dumps require mercury stabilization to prevent air pollution by evaporated mercury, which can be achieved through plant covers. Plants are considered a net sink for atmospheric Hg via incorporation into leaf tissues. However, most studies related to Hg uptake by plants have considered plants exposed to only atmospheric Hg, whereas in the case of tailings dumps, plants are potentially exposed to both soil and atmospheric Hg. The goal of this work is to evaluate the relative contributions of root and atmospheric pathways by growing poplar (Populus trichocarpa X Populus maximowiczii/var Skado) cuttings on either control or polluted substrates and under either n…

Death of mitochondria during programmed cell death of leaf mesophyll cells

2005

The role of plant mitochondria in the programmed cell death (PCD) is widely discussed. However, spectrum and sequence of mitochondrial structural changes during different types of PCD in leaves are poorly described. Pea, cucumber and rye plants were grown under controlled growing conditions. A part of them were sprinkled with ethylene releaser to accelerate cell death. During yellowing the palisade parenchyma mitochondria were attracted to nuclear envelope. Mitochondrial matrix became electron translucent. Mitochondria entered vacuole by invagination of tonoplast and formed multivesicular bodies. Ethephon treatment increased the frequency of sticking of mitochondria to the nuclear envelope …

Secretory Pathway Research: The More Experimental Systems the Better

2012

Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopic expression and the use of dominant mutants can provide insights into protein function.

Proteome analysis of leaves of the desiccation-tolerant grass, Sporobolus stapfianus, in response to dehydration.

2011

Drought and its affects on agricultural production is a serious issue facing global efforts to increase food supplies and ensure food security for the growing world population. Understanding how plants respond to dehydration is an important prerequisite for developing strategies for crop improvement in drought tolerance. This has proved to be a difficult task as all of the current research plant models do not tolerate cellular dehydration well and, like all crops, they succumb to the effects of a relatively small water deficit of -4MPa or less. For these reasons many researchers have started to investigate the usefulness of resurrection plants, plants that can survive extremes of dehydratio…

Development of an efficient regeneration protocol for pear rootstock Pyrodwarf and assessment of somaclonal variability using SSR analysis.

2009

Estudio de perfilación de metabolitos secundarios de extractos vegetales obtenidos mediante técnicas y estrategias no convencionales para el desarrol…

2022

En esta tesis doctoral se desarrolló en el entorno de la economía verde y el reciclaje de residuos alimentarios estudiando diferentes técnicas de extracción no convencionales. En primer lugar, se aislaron polifenoles de matrices complejas producto del desperdicio de alimentos, como son las hojas de Moringa oleifera, mediante extracción asistida por ultrasonidos (UAE). El enfoque de diseño experimental permitió optimizar las mejores condiciones de extracción (MeOH:H2O 50:50, v/v, proporcion 1:60 Sólido/Líquido (S/L), 60°C, 60 min) para obtener un contenido fenólico total (TPC) de 13,4 mg expresados como equivalentes de ácido gálico (GAE)/g de materia seca. Por lo tanto, la caracterización y …

The ER-Membrane Transport System Is Critical for Intercellular Trafficking of the NSm Movement Protein and Tomato Spotted Wilt Tospovirus.

2015

Plant viruses move through plasmodesmata to infect new cells. The plant endoplasmic reticulum (ER) is interconnected among cells via the ER desmotubule in the plasmodesma across the cell wall, forming a continuous ER network throughout the entire plant. This ER continuity is unique to plants and has been postulated to serve as a platform for the intercellular trafficking of macromolecules. In the present study, the contribution of the plant ER membrane transport system to the intercellular trafficking of the NSm movement protein and Tomato spotted wilt tospovirus (TSWV) is investigated. We showed that TSWV NSm is physically associated with the ER membrane in Nicotiana benthamiana plants. An…

Chemical composition of volatile and fixed oils from of Salvia argentea L. (Lamiaceae) growing wild in Sicily.

2015

The chemical compositions of the essential oil and of the non-polar extracts (petroleum ether, dichloromethane) of the aerial parts (flowers, leaves and stems) of Salvia argentea L. were determined by GC-FID and gas chromatography–mass spectrometry analysis. 14-Hydroxy-α-humulene (40.1%) was recognised as the main constituents of the essential oil of S. argentea, together with 1,3,8-p-menthatriene (12.1%), globulol (7.4%) and β-sesquiphellandrene (5.8%). Tritriacontane (9.9% and 14.1%), heptacosane (8.4% and 10.5%), hentriacontane (8.3% and 10.9%), tetradecanal (8.4% and 10.2%) and methyldotriacontane (7.9% and 7.6%) were recognised as the main constituents of the extracts in petroleum …

The determination of polyphenols content in white mulberry leaves.

2008

E. Ciampi, G. Alonzo, A. Sinacori. Cultivation of Mulberry, particularly Morus alba L., was introduced in Europe between the ninth and the twelfth centuries together with sericulture. Mulberry as both Morus alba L. and Morus nigra L. Species, is a rustic plant, well suited for the Mediterranean climate and which is also appreciated for its fruits. Found in the past useful in medicinal applications, is now, from recent studies, indicated as potential health beneficial for the high content in polyphenol substances. Mulberry leaves from 13 varieties, grown in the experimental field of the University of Palermo, Agriculture Faculty, have been investigated for their content in antioxidant substa…

Modifications over time of volatile compounds in coriander (Coriandrum sativum L.)

2007

Coriander (Coriandrum sativum L.) is an annual strongly-smelling herb belonging to the family Apiaceae, which is cultivated all over the world for its aromatic fruits (“seeds”) and, in many Asiatic and South American countries, for its green leaves. The aromatic features of leaves and fruits are sharply different, and the strong smell coming from leaves (many times referred to as a “bug” scent) is not always pleasant to many consumers from western countries. In our work, one Coriander small-seeded biotype has been grown in plastic covered greenhouses, and at given time intervals samples of leaves, flowers and fruits have been analyzed by means of HS-SPME and GC-MS to study the evolution ove…