Search results for " MORPHOLOGY"

Synthesis of polymer materials for use as cell culture substrates

2007

International audience; Up to today, several techniques have been used to maintain cells in culture for studying many aspects of cell biology and physiology. More often, cell culture is dependent on proper anchorage of cells to the growth surface. Thus, poly-L-lysine, fibronectin or laminin are the most commonly used substrates. In this study, electrosynthesized biocompatible polymer films are proposed as an alternative to these standard substrates. The electrosynthesized polymers tested were polyethylenimine, polypropylenimme and polypyrrole. Then, the adhesion, proliferation and morphology of rat neuronal cell lines were investigated on these polymer substrates in an attempt to develop ne…

2019

Abstract Type B Coxsackieviruses (CVBs) belong to the enterovirus genus, and they cause both acute and chronic diseases in humans. CVB infections usually lead to flu-like symptoms but can also result in more serious diseases such as myocarditis, aseptic meningitis and life-threatening multi-organ infections in young infants. Thus, CVBs have long been considered as important targets of future vaccines. We have previously observed CVB1 capsid disintegration and virus concentration decrease with 12-day long formalin inactivation protocol. Here a scalable ion exchange chromatography purification method was developed, and purified CVB1 was inactivated with UV-C or formalin. Virus morphology and …

Evaluation of intra-cultivar variability in Olea europaea L. cv. Leccino using morphological, biochemical and molecular markers

2020

The phenotypic and genotypic variability of olive tree cv. Leccino (Olea europaea L.), an ancient cultivar from North-Central Italy, was investigated using 44 quantitative and qualitative agro-morphological traits, SDS-PAGE of seed proteins (storage proteins and tegument proteins) and molecular markers such as RAPDs (30 primers) and SSRs (8 primers). Fifteen accessions of Olea europaea catalogued as "Leccino" and one certified tree of "Leccino" (LESt) were examined. The plants were clonally propagated and the measuring of all morphological characteristics was conducted over a period of seven years. The ANOVA analysis showed that all accessions were homogenous for many traits while very few …

Morphological and genetic diversity within Pilosella hoppeana aggr. (Asteraceae) in Italy and taxonomic implications

2013

Morphological variation, ploidy level and genetic diversity have been studied on 10 populations of the Pilosella hoppeana aggr. from the Alps, Abruzzo, Calabria and Sicily.Chromosome counts showed that the plants from Abruzzo and those from Sicily are tetraploid (2n = 36); they are assigned to P. hoppeana subsp. macrantha. The plants from the Alps (P. hoppeana subsp. hoppeana) and those from Calabria are diploid. The Calabrian populations, previously included in P. hoppeana subsp. macrantha, are shown to belong to a separate species, P. leucopsilon. The principal component analysis, based on 25 morphological characters, allowed distinguishing clearly four groups. An allozymes study using 10…

Morphological characteristics, microsatellite fingerprinting and determination of incompatibility genotypes of Sicilian sweet cherry cultivars

2007

Sicily has extensive germplasm of diploid sweet cherry (Prunus avium L.) that has not been well studied. In this investigation, 39 cherry accessions, selected from collections and farms, were analysed using molecular markers and characterised for various morphological and other agronomic characters such as flesh colour, fruit size, quality and, in some cases, ripening periods. Thirteen Simple Sequence Repeat (SSR) primer pairs, as well as two primer pairs for the incompatibility (S) locus, which amplified across the first intron of the S-RNase gene and across the intron of the SFB gene, were used in three multiplexed reactions to analyse the accessions. The number of alleles per SSR locus r…

Considerations on the taxonomy and morphology of Microcotyle spp.: redescription of M. erythrini van Beneden & Hesse, 1863 (sensu stricto) (Monog…

2020

AbstractBackgroundMicrocotyle erythrinivan Beneden & Hesse, 1863 (Platyhelminthes: Monogenea) and other closely related species of the genus are often considered as cryptic. Records in hosts other than the type-host with no species confirmation by molecular analyses have contributed to this situation.MethodsGill parasites of five sparid fishes,Boops boops(L.),Pagellus erythrinus(L.),P. acarne(Risso), Dentex dentex(L.) andPagrus pagrus(L.), from the Western Mediterranean off Spain were collected. Specimens ofMicrocotylespp. were characterised both molecularly and morphologically. Partial fragments (domains D1-D3) of the28SrRNA gene and the cytochromecoxidase subunit 1 (cox1) gene were am…

The Bossons glacier protects Europe's summit from erosion

2013

13 pages; International audience; The contrasting efficiency of erosion beneath cold glacier ice, beneath temperate glacier ice, and on ice-free mountain slopes is one of the key parameters in the development of relief during glacial periods. Detrital geochronology has been applied to the subglacial streams of the north face of the Mont-Blanc massif in order to estimate the efficiency of erosional processes there. Lithologically this area is composed of granite intruded at ~303 Ma within an older polymetamorphic complex. We use macroscopic features (on ~10,000 clasts) and U-Pb dating of zircon (~500 grains) to establish the provenance of the sediment transported by the glacier and its subgl…

Monitoring glacier elevation and volume changes with digital photogrammetry and GIS at Gepatschferner glacier, Austria

2006

Digital elevation models of Gepatschferner in Northern Tyrol, Austria were obtained with digital photogrammetry from high altitude stereo photo pairs and by digitizing an analogue topographic glacier map, for 1990 and 1971, respectively. A difference map was calculated to identify regions of glacier elevation increases and decreases corresponding to glacier mass gain and loss. While the glacier tongue below 2600 m showed mainly increases in glacier surface elevation as compared to 1971, the remaining glacier thickness experienced both increases and decreases. The accumulation zone above 3200 m contained several extreme cases of morphological features with altitude changes>+30 m. While the i…

Introduction

2020

Starting from Goethe. Currently, there exists no discipline whose specific boundaries could be defined as MORPHOLOGY. Nevertheless, it is possible to trace out its history in ample terms and to define its scope broadly by understanding it as the place where the semantics of FORMS are defined and where they are connected to a reference IMAGE. The central link in the field of MORPHOLOGY is FORM-IMAGE, and it refers to those dynamics of the FORM and to the DYNAMIC SYSTEMS that have taken hold in late modernity and that continue to grow today.

Dual effect of 1-deoxymannojirimycin on the mannose uptake and on the N-glycan processing of the human colon cancer cell line HT-29.

1990

International audience; 1-Deoxymannojirimycin (dMM), a specific alpha-mannosidase I inhibitor, completely blocks the conversion of Man9-8GlcNAc2 into Man7-5-GlcNAc2 in both differentiated and undifferentiated human adenocarcinoma HT-29 cells. Besides this well known effect on N-glycan trimming, we describe here a novel effect of this inhibitor on the D-[2-3H]mannose uptake that is exclusively observed in differentiated intestinal cells, i.e. cells that display a functional apical brush border membrane. This inhibition of D-[2-3H]mannose uptake was shown to be dose-dependent and reversible. Moreover, using microsomal fractions we showed that this effect depends only on the integrity of the b…