Search results for " Member 3"

showing 6 items of 26 documents

T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients.

2009

Summary There are increased numbers of activated T lymphocytes in the bronchial mucosa of stable chronic obstructive pulmonary disease (COPD) patients. T helper type 17 (Th17) cells release interleukin (IL)-17 as their effector cytokine under the control of IL-22 and IL-23. Furthermore, Th17 numbers are increased in some chronic inflammatory conditions. To investigate the expression of interleukin (IL)-17A, IL-17F, IL-21, IL-22 and IL-23 and of retinoic orphan receptor RORC2, a marker of Th17 cells, in bronchial biopsies from patients with stable COPD of different severity compared with age-matched control subjects. The expression of IL-17A, IL-17F, IL-21, IL-22, IL-23 and RORC2 was measure…

MaleTranslational StudiesReceptors Retinoic Acidmedicine.medical_treatmentImmunologyautoimmunity bronchial biopsies emphysema neutrophilsInflammationBronchiInterleukin-23Polymerase Chain ReactionStatistics NonparametricPulmonary Disease Chronic ObstructiveAutoimmunity bronchial biopsies emphysema neutrophils pathologymedicineInterleukin 23Immunology and AllergyHumansRNA MessengerAgedDNA PrimersCOPDAnalysis of VarianceMucous MembraneReceptors Thyroid Hormonebusiness.industryInterleukinsRespiratory diseaseInterleukin-17SmokingInterleukinT-Lymphocytes Helper-InducerMiddle AgedNuclear Receptor Subfamily 1 Group F Member 3medicine.diseaseImmunohistochemistryrespiratory tract diseasesRespiratory Function TestsCytokineCase-Control StudiesImmunologyFemaleInterleukin 17medicine.symptombusinessCD8
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Functional cysteine-less subunits of the transporter associated with antigen processing (TAP1 and TAP2) by de novo gene assembly

2002

AbstractWithin the adaptive immune system the transporter associated with antigen processing (TAP) plays a pivotal role in loading of peptides onto major histocompatibility (MHC) class I molecules. As a central tool to investigate the structure and function of the TAP complex, we created cysteine-less human TAP subunits by de novo gene synthesis, replacing all 19 cysteines in TAP1 and TAP2. After expression in TAP-deficient human fibroblasts, cysteine-less TAP1 and TAP2 are functional with respect to adenosine triphosphate (ATP)-dependent peptide transport and inhibition by ICP47 from herpes simplex virus. Cysteine-less TAP1 and TAP2 restore maturation and intracellular trafficking of MHC c…

Models MolecularBiophysicsBiological Transport ActiveBiologyMajor histocompatibility complexTransfectionBiochemistryCell Linechemistry.chemical_compoundAdenosine TriphosphateStructural BiologyATP Binding Cassette Transporter Subfamily B Member 3Cysteine-scanning mutagenesisMHC class IGeneticsHumansCysteineATP Binding Cassette Transporter Subfamily B Member 2Molecular BiologyAntigen PresentationAntigen processingHistocompatibility Antigens Class ICell BiologyTransporter associated with antigen processingMolecular biologyRecombinant ProteinsCell biologyProtein SubunitschemistryAmino Acid SubstitutionAntigen processingPeptide transportMembrane proteinbiology.proteinAdenosine triphosphate-binding cassette transporterTAP2ATP-Binding Cassette TransportersTAP1Adenosine triphosphateFEBS Letters
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Analysis of the MHC Class I Antigen Presentation Machinery in Human Embryonal Carcinomas: Evidence for Deficiencies in TAP, LMP and MHC Class I Expre…

1998

The expression of the major histocompatibility complex (MHC) class I antigens is suppressed in early post-implantation embryonic cells as well as in embryonal carcinoma (EC) cells, but could be upregulated by treatment with interferon (IFN)-gamma or retinoic acid. In a number of human and murine tumours, defects in the expression of the different components of the MHC class I antigen processing machinery, such as the proteasomal subunits LMP-2 and LMP-7 and the peptide transporters TAP-1 and TAP-2, account for impaired MHC class I surface expression. Here, we analysed the constitutive and IFN-gamma regulated mRNA and protein expression of the LMP, TAP and MHC class I molecules in the human …

Proteasome Endopeptidase ComplexCD74HIV AntigensImmunologyCD1CytomegalovirusInterferon-gammaATP Binding Cassette Transporter Subfamily B Member 3Multienzyme ComplexesCarcinoma EmbryonalMHC class ITumor Cells CulturedHumansATP Binding Cassette Transporter Subfamily B Member 2Antigens ViralAntigen PresentationbiologyAntigen processingMHC class I antigenHistocompatibility Antigens Class ITemperatureGeneral MedicineTransporter associated with antigen processingMHC restrictionMolecular biologyUp-RegulationCysteine EndopeptidasesProtein Biosynthesisbiology.proteinATP-Binding Cassette TransportersPeptidesCD8Scandinavian Journal of Immunology
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Functional deficiencies of components of the MHC class I antigen pathway in human tumors of epithelial origin

2000

An association between oncogenic transformation and repression of different components of the MHC class I antigen processing machinery (APM) have been described in murine model systems. In order to discover whether a similar correlation exists, human tumor cell lines of distinct histology with altered ras protein were analyzed for the expression of APM components utilizing RT-PCR and Western blot analyses. A heterogeneous expression pattern of MHC class I antigens, TAP peptide transporter, proteasome subunits, proteasome activator PA28 and the chaperones calnexin, calreticulin as well as tapasin was displayed by these tumor cell lines. Single or combined deficiencies in the expression and/o…

Proteasome Endopeptidase ComplexGene ExpressionInterferon-gammaMiceTapasinATP Binding Cassette Transporter Subfamily B Member 3Multienzyme ComplexesCalnexinGene expressionMHC class ITumor Cells CulturedAnimalsHumansNeoplasms Glandular and EpithelialATP Binding Cassette Transporter Subfamily B Member 2DNA PrimersAntigen PresentationTransplantationBase SequencebiologyAntigen processingMHC class I antigenHistocompatibility Antigens Class IProteinsHematologyTransporter associated with antigen processingRecombinant ProteinsCell biologyCysteine EndopeptidasesGenes rasMutationCancer researchbiology.proteinATP-Binding Cassette TransportersCalreticulinBone Marrow Transplantation
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Features of TAP-independent MHC class I ligands revealed by quantitative mass spectrometry.

2008

TAP is responsible for transferring cytosolic peptides into the ER, where they can be loaded onto MHC molecules. Deletion of TAP results in a drastic reduction of MHC class I surface expression and alters the presented peptide pattern. This key molecule in antigen processing is tackled by several viruses and lost in some tumors, rendering the altered cells less vulnerable to T cell-based immune surveillance. Using the TAP-deficient cell line LCL721.174 and its TAP-expressing progenitor cell line LCL721.45, we identified and quantified more than 160 HLA ligands, 50 of which were presented TAP-independently. Peptides which were predominantly presented on the TAP-deficient LCL721.174 cell line…

Proteasome Endopeptidase ComplexImmunologyAntigen presentationEpitopes T-LymphocyteGene ExpressionHuman leukocyte antigenCysteine Proteinase InhibitorsProtein Sorting SignalsMajor histocompatibility complexCell LineAntigenATP Binding Cassette Transporter Subfamily B Member 3HLA AntigensTandem Mass SpectrometryMHC class IHLA-A2 AntigenImmunology and AllergyHumansAmino Acid SequenceATP Binding Cassette Transporter Subfamily B Member 2Antigen PresentationbiologyHLA-A AntigensAntigen processingHistocompatibility Antigens Class IProteinsTransporter associated with antigen processingMHC restrictionMolecular biologyPeptide FragmentsCell biologyHLA-B AntigensIsotope Labelingbiology.proteinATP-Binding Cassette TransportersProteasome InhibitorsGene DeletionProtein BindingEuropean journal of immunology
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Development and characterization of mouse anti-human LMP2, LMP7, TAP1 and TAP2 monoclonal antibodies.

2008

Low molecular mass polypeptides (LMP) 2 and LMP7 and transporter associated with antigen processing (TAP) subunits TAP1 and TAP2 play a crucial role in antigen processing and cell surface expression of HLA class I molecules. Since monoclonal antibodies (mAb) to these molecules will facilitate the analysis of their expression, structure and function in normal and transformed cells, in the present study we have developed these reagents. Specifically anti-LMP2 and LMP7 mAb were generated from BALB/c mice immunized with specific peptides, and anti-TAP1 and TAP2 mAb from BALB/c mice immunized with respective recombinant proteins. mAb VF101-39F7 and VF101-39G5 were shown to be specific for LMP2, …

Proteasome Endopeptidase Complexmedicine.drug_classRecombinant Fusion ProteinsImmunologyAntigen presentationBiologyMonoclonal antibodyBiochemistrylaw.inventionCell LineMicelawATP Binding Cassette Transporter Subfamily B Member 3Antibody SpecificityHLA AntigensMultienzyme ComplexesGeneticsmedicineImmunology and AllergyAnimalsHumansATP Binding Cassette Transporter Subfamily B Member 2DNA PrimersSkinAntigen PresentationMice Inbred BALB CHybridomasImmunoperoxidaseBase SequenceAntigen processingAntibodies MonoclonalProteinsGeneral MedicineTransporter associated with antigen processingMolecular biologyImmunohistochemistryCysteine EndopeptidasesCell cultureMonoclonalRecombinant DNAATP-Binding Cassette TransportersFemaleIndicators and ReagentsTissue antigens
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