Search results for " Mesoangioblast"
showing 10 items of 21 documents
Porous PLLA scaffolds are optimal substrates for internal colonization by A6 mesoangioblasts and immunocytochemical analyses
2009
In the present paper, mouse mesoangioblasts were seeded onto bidimensional matrices and within three-dimensional porous scaffolds of poly(L-lactic acid) (PLLA), in the presence or absence of type I collagen coating, observed under the scanning electron microscope, and tested for their adhesion, survival and proliferation. Immunolocalization of Hsp70, an abundant and ubiquitous intracellular protein in these cells, was also performed in sectioned cell-containing scaffolds under the confocal fluorescence microscope to check whether "in situ" analysis of intracellular constituents was feasible. The data obtained show that PLLA films allow direct cell adhesion and represent an optimal support f…
Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangio…
2010
Certain proteins, including fibroblast growth factor-2 (FGF-2) and matrix metalloproteinase-9 (MMP-9), have proved very effective in increasing the efficacy of mesoangioblast stem cell therapy in repairing damaged tissue. We provide the first evidence that mouse mesoangioblast stem cells release FGF-2 and MMP-9 in their active form through the production of membrane vesicles. These vesicles are produced and turned over continuously, but are stable for some time in the extracellular milieu. Mesoangioblasts shed membrane vesicles even under oxygen tensions that are lower than those typically used for cell culture and more like those of mouse tissues. These findings suggest that mesoangioblast…
Ku factor is responsible of Hsp70 basal transcription in mouse mesoangioblasts
2008
Paracrine effect of membrane vesicles released by mouse mesoangioblast stem cells on non correlated cell types
2016
Introduction Mouse mesoangioblasts are vessel-associated multipotent progenitor stem cells, which are able to differentiate into different mesodermal cell types. In our previous paper we have demonstrated that these cells are able to shed in the extracellular environment membrane vesicles (EV), which contain both structural proteins and biological factors such as FGF2 and the two gelatinases MMP2/9. EV represent an important mediator of cell-to-cell communication and are involved in both autocrine and paracrine signalling. Interestingly, there is a bidirectional signalling exchange between stem cell EV and damaged cells. In particular, EV from injured cells can reprogram stem cells to acqui…
Mouse mesoangioblast behaviour when subjected to cellular stress
2009
Selezione di cloni più resistenti allo stress ossidativo dalla popolazione di cellule staminali mesoangioblasti
2012
Paracrine roles of extracellular vesicles released by mouse mesoangioblasts
2017
Extracellular vesicles (EV) represent an important mediator of cell-to-cell communication and are involved in both autocrine and paracrine signaling, with a critical role in a number of physiological and pathological conditions.1 The bioactive molecules contained within EV simultaneously activate several different pathways resulting in the synergistic stimulation of target cells. The discovery and characterization of EV have added a novel understanding to regenerative medicine, namely the finding that stem cells are an abundant source of EV.1-2 A6 mouse mesoangioblasts, vessel-associated multipotent progenitor stem cells that are capable of differentiating into different mesodermal cell typ…
Paracrine effect of membrane vesicles released by mouse mesoangioblast stem cells
2016
Introduction: Mouse mesoangioblasts are vessel-associated multipotent progenitor stem cells, which are able to differentiate into different mesodermal cell types. In our previous paper, we have demonstrated that mesoangioblasts are able to shed in the extracellular environment membrane vesicles (EVs), which contain both structural proteins and biological factors such as FGF2 and the two gelatinases MMP2/9. We investigated whether these EV interact in a paracrine way with other cell types different from mesoangioblasts, and eventually the effects of this interaction. Methods: Mesoangioblast EVs were collected from conditioned media by ultracentrifugation. Total mRNAs from mesoangioblasts and…
Mouse A6 stem cells release active FGF-2 into extracellular space through plasma membrane vesicles
2007
In this study, mouse mesoangioblasts were seeded onto bidimensional matrices within three-dimensional porous scaffolds of poly (L-lactic acid) (PLLA), in the presence or absence of a type I collagen coating. The cells were observed under a scanning electron microscope and tested for their adhesion, survival and proliferation. Immunolocalization of heat shock protein (Hsp) 70, an abundant and ubiquitous intracellular protein in these cells, was also performed in sectioned cell-containing scaffolds under a confocal fluorescence microscope to determine if in situ analysis of intracellular constituents was feasible. The data show that PLLA films allow direct cell adhesion and represent an optim…