Search results for " Methods"

showing 10 items of 4102 documents

Analysis of thiamine transporter genes in sporadic beriberi

2014

Abstract Objective Thiamine or vitamin B 1 deficiency diminishes thiamine-dependent enzymatic activity, alters mitochondrial function, impairs oxidative metabolism, and causes selective neuronal death. We analyzed for the first time, the role of all known mutations within three specific thiamine carrier genes, SLC19 A2, SLC19 A3 , and SLC25 A19 , in a patient with atrophic beriberi, a multiorgan nutritional disease caused by thiamine deficiency. Methods A 44-year-old male alcoholic patient from Morocco developed massive bilateral leg edema, a subacute sensorimotor neuropathy, and incontinence. Despite normal vitamin B 1 serum levels, his clinical picture was rapidly reverted by high-dose in…

AdultMalemedicine.medical_specialtySLC19 A- SLC25 A19SLC19 AEndocrinology Diabetes and MetabolismGene mutationBeriberimedicine.disease_causeMitochondrial Membrane Transport Proteinslaw.inventionBeriberilawInternal medicineGenotypemedicineThiamine transporterObjective: Thiamine or vitamin B1 deficiency diminishes thiamine-dependent enzymatic activity alters mitochondrial function impairs oxidative metabolism and causes selective neuronal death. We analyzed for the first time the role of all known mutations within three specific thiamine carrier genes SLC19 A2 SLC19 A3 and SLC25 A19 in a patient with atrophic beriberi a multiorgan nutritional disease caused by thiamine deficiency. Methods: A 44-year-old male alcoholic patient from Morocco developed massive bilateral leg edema a subacute sensorimotor neuropathy and incontinence. Despite normal vitamin B1 serum levels his clinical picture was rapidly reverted by high-dose intramuscular thiamine treatment suggesting a possible genetic resistance. We used polymerase chain reaction followed by amplicon sequencing to study all the known thiamine-related gene mutations identified within the Human Gene Mutation Database. Results: Thirty-seven mutations were tested: 29 in SLC19 A2 6 in SLC19 A3 and 2 in SLC25 A19. Mutational analyses showed a wild-type genotype for all sequences investigated. Conclusion: This is the first genetic study in beriberi disease. We did not detect any known mutation in any of the three genes in a sporadic dry beriberi patient. We cannot exclude a role for other known or unknown mutations in the same genes or in other thiamine-associated genes in the occurrence of this nutritional neuropathy.HumansThiamineGenePolymerase chain reactionGeneticsMutationNutrition and DieteticsbiologyMembrane Transport ProteinsThiamine Deficiencymedicine.diseaseAlcoholismEndocrinologyMutationbiology.proteinThiamineMutations
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Comparative effect of tacrolimus 0.1% ointment and clobetasol 0.05% ointment in patients with oral lichen planus.

2008

Background Oral lichen planus (OLP) is considered to be an autoimmune disease of unknown aetiology that affects the mucosae, especially the oral cavity. Objective We compared tacrolimus 0.1% ointment and clobetasol 0.05% ointment for the treatment of OLP. Patients and methods A total of 32 patients (20 females and 12 males; all white, Italian origin, mean age of 43.6+/-18.4 years; 16 patients per treatment group) were treated with tacrolimus or clobetasol ointment for 4 weeks in a randomized, double-blind, clinical trial. Pain severity, burning sensation, and mucosal lesion extension were assessed using a four-point scale. Results At the end of the treatment period, symptom scores were sign…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina Internamedicine.drug_classAnti-Inflammatory Agentstacrolimus ointment; clobetasol ointment; oral lichen planuslaw.inventionOintment Basesoral lichen planusRandomized controlled triallawOral administrationFacial PainmedicineHumanstacrolimus ointmentSalivatacrolimusbusiness.industryclobetasol ointmentclobetasol; tacrolimus; oral lichen planusmedicine.diseaseDermatologyTacrolimusCalcineurinstomatognathic diseasesclobetasolOintment BasesPeriodonticsCorticosteroidOral lichen planusFemaleClobetasol propionatebusinessEpidemiologic MethodsImmunosuppressive Agentsmedicine.drugLichen Planus Oral
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Progression of untreated mild thoracic Scheuermann's kyphosis – Radiographic and functional assessment after mean follow-up of 46 years

2017

There is no data available on the radiographic development of the Scheuermann's deformity. Our purpose was to investigate radiographic deformity progression and the relation between kyphosis progression and clinical outcome in patients with untreated Scheuermann's kyphosis.Thoracic kyphosis (Th4-Th12) was measured from standing lateral radiographs in 19 patients at baseline and after mean 46-year follow-up. Mean age at baseline was 19.2 and at follow-up 64.7 years. At follow-up, height, weight, hand grip strength, and hamstring tightness were measured, and sit-to-stand and walking tests were performed. Additionally general health and quality of life questionnaires were administered.The mean…

AdultMalemedicine.medical_specialtyTime FactorsAdolescentScheuermann's kyphosisRadiographyKyphosisScheuermann DiseasepatientsspineThoracic VertebraeYoung Adult03 medical and health sciencesGrip strength0302 clinical medicineQuality of lifeDeformitymedicineHumansScheuermanns kyfosOrthopedics and Sports MedicineRange of Motion Articularradiologi030222 orthopedicsradiographersbusiness.industryta3141Recovery of Functionfollow-up studyMiddle Agedmedicine.diseaseSurgeryRadiographymedicine.anatomical_structureThoracic vertebraeDisease ProgressionorthopedicsFemaleSurgerybehandlingsresultatmedicine.symptomRange of motionbusiness030217 neurology & neurosurgeryHamstringFollow-Up Studiestreatment methodsJournal of Orthopaedic Science
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Cancer-specific survival after radical cystectomy and standardized extended lymphadenectomy for node-positive bladder cancer: prediction by lymph nod…

2009

OBJECTIVE To investigate the associations between different overall or topographically restricted lymph node (LN) variables and cancer-specific survival (CSS) after radical cystectomy (RC) and extended LN dissection (LND) with curative intent in patients with LN-positive bladder cancer. PATIENTS AND METHODS Between 2001 and 2006, 152 patients had RC with standardized extended LND for bladder cancer with curative intent. Patients with positive LNs were stratified according to the median of the LN variables (LNs removed, number of positive LNs, LN density). CSS was related to overall and topographically restricted LN variables, e.g. different levels of LND, and relationships were tested by un…

AdultMalemedicine.medical_specialtyUrologymedicine.medical_treatmentUrologyCystectomyCystectomymedicineCarcinomaHumansLymph nodeAgedAged 80 and overCarcinoma Transitional CellBladder cancerbusiness.industryHazard ratioCancerMiddle AgedPrognosismedicine.diseaseConfidence intervalSurgeryTreatment Outcomemedicine.anatomical_structureUrinary Bladder NeoplasmsLymphatic MetastasisLymph Node ExcisionFemaleLymphadenectomyEpidemiologic MethodsbusinessBJU International
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Liver assessment and biopsy in patients with marked coagulopathy: value of mini-laparoscopy and control of bleeding.

2003

Evaluation of liver disease in patients with a high risk of postbiopsy bleeding presents a diagnostic challenge. Mini-laparoscopy offers the possibility of coagulation of biopsy site and the additional advantage of macroscopic liver assessment. We wished to assess the value and safety of mini-laparoscopy with guided biopsy as a diagnostic approach in patients in whom percutaneous liver biopsy is considered contraindicated because of a marked coagulopathy.We investigated 61 consecutive patients with marked coagulopathy (prolonged international normalized ratio1.5, thrombocytopenia50/nl, or both; von Willebrand's disease/hemophilia). Diagnostic mini-laparoscopy with visually guided liver biop…

AdultMalemedicine.medical_specialtyValidation studyAdolescentBiopsyHemorrhageBiopsymedicineCoagulopathyHumansIn patientLaparoscopyChildBiopsy methodsAgedAged 80 and overHepatologymedicine.diagnostic_testbusiness.industryLiver DiseasesMini laparoscopyGastroenterologyInfantReproducibility of ResultsEquipment DesignBlood Coagulation DisordersMiddle Agedmedicine.diseaseLaparoscopesSurgeryEndoscopyChild PreschoolFeasibility StudiesFemaleLaparoscopyRadiologybusinessThe American journal of gastroenterology
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Predictive role of capillaroscopic skin ulcer risk index in systemic sclerosis: A multicentre validation study

2012

IntroductionThe early detection of systemic sclerosis (SSc) patients at high risk of developing digital ulcers could allow preventive treatment, with a reduction of morbidity and social costs. In 2009, a quantitative score, the capillaroscopic skin ulcer risk index (CSURI), calculated according to the formula ‘D×M/N2’, was proposed, which was highly predictive of the appearance of scleroderma digital ulcers within 3 months of capillaroscopic evaluation.ObjectivesThis multicentre study aims to validate the predictive value and reproducibility of CSURI in a large population of SSc patients.MethodsCSURI was analysed in 229 unselected SSc patients by nailfold videocapillaroscopy (NVC). All pati…

AdultMalemedicine.medical_specialtyValidation studypredictive model; capillaroscopic skin ulcer; systematic scelorisImmunologyRisk AssessmentGeneral Biochemistry Genetics and Molecular BiologySclerodermaMicroscopic AngioscopySclerodermaFingersRheumatologyInternal medicineRisk indexEpidemiologySkin UlcermedicineImmunology and AllergyHumansAgedSkinScleroderma SystemicReceiver operating characteristicbusiness.industrySystemicArea under the curveSkin ulcerMiddle Agedmedicine.diseaseConnective tissue diseaseSurgeryCapillariesEarly DiagnosisNailsFemalemedicine.symptombusinessEpidemiologic MethodsAlgorithms
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Mid-Term Effectiveness of Behavioral and Psychodynamic Inpatient Treatments of Severe Obesity Based on a Randomized Study

2006

<i>Background:</i> The objective of this study was to compare the effectiveness of psychodynamic and behavioral inpatient treatments of severely obese patients regarding weight and distress. <i>Methods:</i> In a longitudinal study obese patients (body mass index, BMI ≧35) were randomly assigned to behavioral or to psychodynamic inpatient treatment. Mostly female (n = 267; 85%) obese patients with psychiatric and somatic comorbidity (age 20–64 years, BMI 35–74) were examined with standardized self-report scales on distress (SCL-90R), interpersonal problems (Inventory of Interpersonal Problems), eating behavior (Fragebogen zum Essverhalten) and body image (Fragebogen z…

AdultMalemedicine.medical_specialtymacromolecular substancesBody weightSeverity of Illness IndexBody Mass Indexlaw.inventionPsychiatric comorbidityRandomized controlled trialBehavior TherapylawSurveys and QuestionnairesmedicineHumansInterpersonal RelationsObesityBulimia NervosaPsychiatryApplied PsychologyApplication methodsbusiness.industryFollow up studiesGeneral MedicineMiddle AgedSevere obesityPsychodynamicsmedicine.diseaseObesityHospitalizationPsychotherapyPsychiatry and Mental healthClinical PsychologyFemalebusinessFollow-Up StudiesPsychotherapy and Psychosomatics
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Survival trends and predictors of mortality in severe pelvic trauma: estimates from the German Pelvic Trauma Registry Initiative.

2010

Abstract Study objective To determine longitudinal trends in mortality, and the contribution of specific injury characteristics and treatment modalities to the risk of a fatal outcome after severe and complex pelvic trauma. Methods We studied 5048 patients with pelvic ring fractures enrolled in the German Pelvic Trauma Registry Initiative between 1991 and 1993, 1998 and 2000, and 2004 and 2006. Complete datasets were available for 5014 cases, including 508 complex injuries, defined as unstable fractures with severe peri-pelvic soft tissue and organ laceration. Multivariable mixed-effects logistic regression analysis was employed to evaluate the impact of demographic, injury- and treatment-a…

AdultMalemedicine.medical_specialtymedicine.medical_treatmentAbdominal InjuriesFractures BoneYoung AdultFracture FixationLaparotomyGermanyFracture fixationEpidemiologymedicineHumansRegistriesYoung adultPelvic BonesSurvival analysisGeneral Environmental ScienceAgedbusiness.industryMultiple TraumaAccidents TrafficOdds ratioMiddle AgedVascular System InjuriesSurvival AnalysisConfidence intervalSurgeryEmergency medicineGeneral Earth and Planetary SciencesInjury Severity ScoreFemalebusinessEpidemiologic MethodsInjury
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Associations of Physical Fitness and Body Composition Characteristics With Simulated Military Task Performance

2017

Pihlainen, K, Santtila, M, Hakkinen, K, and Kyrolainen, H. Associations of physical fitness and body composition characteristics with simulated military task performance. J Strength Cond Res 32(4): 1089-1098, 2018-The purpose of this study was to evaluate the associations of physical fitness and body composition characteristics with anaerobic endurance performance, tested in the combat load using the occupationally relevant military simulation test (MST). Eighty-one male soldiers, deployed to a crisis management operation in the Middle East, volunteered for the study and participated in a test battery consisting measurements of muscle strength, body composition characteristics, endurance ca…

AdultMaleobstacle courseMultivariate statisticsperformance (physical capacity)Physical fitnessPhysical Therapy Sports Therapy and RehabilitationFat mass03 medical and health sciencesYoung Adult0302 clinical medicinesoldiersanaerobic capacityStatisticsTask Performance and AnalysisHumansOrthopedics and Sports Medicine030212 general & internal medicineCorrelational analysisMuscle StrengthsotilaatMuscle Skeletalta315Simulation TrainingMathematicskehonkoostumussuorituskykybody compositionanaerobinen suorituskykybusiness.industry030229 sport sciencesGeneral MedicineComposition (combinatorics)Middle Agedfyysinen kuntoMilitary PersonnelMuscle powerAssessment methodsExercise TestPhysical Endurancephysical fitnessbusinesshuman activitiesAnaerobic exerciseJournal of Strength and Conditioning Research
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Self-Other Differentiation Scale: Dimensionality, IRT Parameterization, and Measurement Invariance

2018

The Self-Other Differentiation Scale (Olver, Aries, & Batgos, 1989) is a self-report instrument assessing the experience of a separate sense of self from others. The authors aimed to examine its dimensionality, reliability, and measurement invariance across gender. It was completed by 348 participants (48% men) from 17 to 30 years old in Study 1, 348 participants (40% men) from 18 to 28 years old in Study 2, and 1,068 participants (49% men) from 17 to 28 years old in Study 3. The results supported the hypothesis of just one factor underlying the scale; they also showed an appropriate internal consistency and a partial measurement invariance across gender. Results also showed evidence fo…

AdultMalescale validationAdolescentPsychometricsScale (ratio)PsychometricsSelf-conceptPsychology of self050109 social psychologyself-other differentiationMeasurement invariance; multiple-group categorical confirmatory factor analysis; scale validation; self-other differentiation; Developmental and Educational Psychology; Clinical Psychology; Life-span and Life-course StudiesYoung Adult0504 sociologyDevelopmental and Educational PsychologyHumans0501 psychology and cognitive sciencesMeasurement invarianceLife-span and Life-course StudiesReliability (statistics)Measurement invariancemultiple-group categorical confirmatory factor analysis05 social sciencesSelf otherReproducibility of Results050401 social sciences methodsSelf ConceptClinical PsychologyFemaleSelf ReportPsychologyCognitive psychologyCurse of dimensionalityThe Journal of Genetic Psychology
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