Search results for " Microbial"

Two changes of the same nucleotide confer resistance to diuron and antimycin in the mitochondrial cytochrome b gene of Schizosaccharomyces pombe

1988

AbstractDiuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) and antimycin, both inhibitors of mitochondrial respiration, block electron flow between cytochromes b and c1. Mutants resistant to either drug have been selected using Schizosaccharomyces pombe strains with an extrachromosomally inherited mutator. In analogy to Saccharomyces cerevisiae these mutational sites were assumed to map in the cytochrome b gene. DNA sequence analysis showed that two changes in the same nucleotide are responsible for resistance to antimycin and diuron. Analysis of resistant and sensitive progeny of crosses between the mutants and the wild type confirmed the correlation between mutational alteration and resista…

Plasmid-Mediated Resistance to Antimicrobial Agents among Listeriae

1998

The resistance to 14 antiseptic-disinfectant and dye compounds of 208 strains of Listeria (132 L. monocytogenes, 63 L. innocua, 8 L. seeligeri, 1 L. ivanovii, 1 L. welshimeri, and 3 Listeria spp.) was tested by the agar-dilution procedure. The Listeria strains were isolated from different varieties of foods, environments of cheese dairies, humans, and wild birds. A total of 14 (6.7%) Listeria strains (12 L. monocytogenes and 2 L. innocua) were resistant to benzalkonium chloride, hexamidine diisethionate, and ethidium bromide. This multiple resistance was observed more frequently from strains of Listeria spp. detected on carcasses of poultry (47%) than strains isolated from human listeriosis…

Epidemiological analysis of Salmonella enterica from beef sampled in the slaughterhouse and retailers in Dakar (Senegal) using pulsed-field gel elect…

2007

Seventy-eight isolates of Salmonella spp. isolated from beef sampled from the official city slaughterhouse and from retailers in Dakar, Senegal were analyzed using serotyping, antimicrobial testing and macrorestriction profiling by Pulsed-Field Gel Electrophoresis (PFGE). These analyses were done to identify clonal relationships and potential transmission routes in beef channel. XbaI macrorestriction allowed defining 17 genotypes among the six main analyzed serotypes: Salmonella bredeney (3 genotypes), S. muenster (6), S. waycross (1), S. corvallis (3), S. kentucky (1) and S. brandenburg (3). The cross analysis of PFGE profiles and origin of the beef samples reveals a wide range of contamin…

Mucilage-Based and Calcium Ascorbate Edible Coatings Improve Postharvest Quality and Storability of Minimally Processed Cactus Pear Fruit Stored unde…

2022

The minimally processed fruit and vegetable industry showed rapid growth worldwide, primarily due to the increasing consumer need for ready-to-eat fresh products characterized by high nutritional, sensory and healthy value. The postharvest life of peeled cactus pear fruits is relatively short, due to the processing operations that affect fruit integrity and cause metabolic disfunctions, as well as pulp browning, microbial growth, loss of firmness, off-flavor development, and nutraceutical value loss. In this study, we investigated the effects of mucilage-based (OFI) and calcium ascorbate edible coating on minimally processed cactus pear summer-ripening fruit, cold stored under passive atmos…

Chemical, biochemical and microbial diversity through a Pachic Humudept profile in a temperate upland grassland

2013

There is great interest in understanding the factors that drive soil microbial activity and community composition in upland grassland ecosystems. We investigated the role of vertical gradients of chemical properties and various soluble C and N pools on soil microbial community structure by using a combination of chemical and biochemical methods coupled with PCR-denaturing gradient gel electrophoresis (DGGE) community fingerprinting and phospholipid fatty acid (PLFA) profiling. Soil samples were collected at increasing depth from a temperate upland grassland. Soil organic matter-related pools (total organic C and total Kjeldahl N) and functionally related active pools (microbial biomass C an…

Management practices to preserve soil organic matter in semiarid mediterranean environment

2012

Organic matter (SOM) is a key constituent of soil as it is a “revolving nutrient fund” and improves soil structure, maintains crop production and minimizes erosion. In semiarid environments, the major problem for sustainable farming systems is the continuous decline of SOM towards levels too low for agricultural purposes. Furthermore, SOM is per se a dynamic entity. Its quantity and quality depend on numerous factors including climate, vegetation type, nutrient availability, disturbance, land use and management practices. In particular, soil organic carbon (SOC) stocks in Mediterranean semiarid agrosystems are constrained by 1) limited C inputs because of low precipitation and high evapotra…

From wastes to resources: citrus hydrolatesas natural biostimulants of soil microorganisms

The hydrolates result from the industrial extraction process of the essential oils through cold pressing of the citrus peels. Today, they are considered a waste to be disposed of. However, due to the presence of water soluble compounds (sugars, polyphenols, acids), hydrolates could be reused instead of being, due to the high economic burden, a problem in the disposal of the same, charged to the company.The aim of this work was to evaluate the effects of citrus hydrolate when directly applied as irrigation water on soil microbial biomass, activity and structure community. The soil used for the experiment was collected from the topsoil (0-10 cm) of a citrus orchard, air-dried and sieved at 2 …

The effects of the traditional producing system on physicochemical, microbial and sensory properties of Caciocavallo Palermitano cheese

2015

Caciocavallo Palermitano (CP) is a traditional cheese made with raw milk from cows of Sicilian local breeds fed pasture-based diets in extensive farms (EXT), processed by an artisanal technology (ART) based on wooden tools and the action of native microflora. CP is obtained also in intensive farms (INT) where milk from cows of specialized breeds fed dry diets is transformed by advanced procedures (ADV) using a stainless steel equipment and lactic acid starter cultures. This research was planned to investigate the changes in cheese properties due to production system and ripening., Milk was collected 3 times from an EXT and an INT farm and processed in ART and ADV conditions. The 12 produced…

Il caglio in pasta utilizzato per la produzione di formaggi tradizionali siciliani: indagine microbiologica

2014

Bioaerosol

2022

Cultural heritage constitutive materials can provide excellent substrates for microbial colonisation, highly influenced by thermo-hygrometric parameters. In cultural heritage-related environments, a detrimental microbial load may be present both on artworks surface and in the aerosol. Confined environments (museums, archives, deposits, caves, hypogea) are characterised by peculiarstructures and different thermo-hygrometric conditions, influencing the development of a wide range of microbial species, able to induce artefact biodeterioration and to release biological particles in the aerosol (spores, cellular debrides, toxins, allergens) potentially dangerous for the human health (visitors/us…