Search results for " Mito"

Mitochondrial complex I impairment in leukocytes from polycystic ovary syndrome patients with insulin resistance.

2009

Insulin resistance is a feature of polycystic ovary syndrome (PCOS) and is related to mitochondrial function.Our objective was to assess mitochondrial function by evaluating mitochondrial oxygen (O(2)) consumption, reactive oxygen species (ROS) production, levels of glutathione (GSH), the oxidized glutathione/GSH ratio, TNFalpha levels, and membrane potential. Additionally, we have evaluated mitochondrial complex I as a target of the oxidative stress responsible for PCOS in polymorphonuclear cells.This was a prospective controlled study conducted in an academic medical center.The study population consisted of 20 lean reproductive-age women with PCOS and 20 body composition-matched controls.…

Interleukin 12 enhances deficient HCV-antigen-induced Th1-type immune response of peripheral blood mononuclear cells

1998

The aim of this study was to examine the possible immunomodulating effects of rhIL-12 on the immune response induced by different hepatitis C virus (HCV) antigens. Freshly isolated peripheral blood mononuclear cells (PBMC) of 33 patients with chronic HCV infection were stimulated with optimal concentrations of antigens from the NS3, NS4, NS5, and core region of HCV in the absence or presence of interleukin12 (IL-12). Stimulation by α-CD3 + α-CD28, lipopolysaccharide (LPS), and pokeweed mitogen (PWM) were used as controls. Proliferation and cytokine production were determined by 3H-thymidine uptake and enzyme-linked immunosorbent assay (ELISA) after 72 hr. After stimulation with antigen or a…

POLYPHENOLS FROM RED WINE MODULATE IMMUNE RESPONSIVENESS: BIOLOGICAL AND CLINICAL SIGNIFICANCE.

2008

Many studies have been conducted on the effects of red wine polyphenols on certain diseases, primarily, coronary heart disease (CHD) and, in this respect, evidence has been demonstrated that intake of red wine is associated with a reduction of CHD symptomatology. In this framework, the purpose of this review is to illustrate the effects of polyphenols on immune cells from human healthy peripheral blood. Data will show that polyphenols are able to stimulate both innate and adaptive immune responses. In particular, the release of cytokines such as interleukin (IL)-12, interferon (IFN)-gamma, and IL-10 as well as immunoglobulins may be important for host protection in different immune related …

Absence of the lactase-persistence-associated allele in early Neolithic Europeans.

2007

Lactase persistence (LP), the dominant Mendelian trait conferring the ability to digest the milk sugar lactose in adults, has risen to high frequency in central and northern Europeans in the last 20,000 years. This trait is likely to have conferred a selective advantage in individuals who consume appreciable amounts of unfermented milk. Some have argued for the “culture-historical hypothesis,” whereby LP alleles were rare until the advent of dairying early in the Neolithic but then rose rapidly in frequency under natural selection. Others favor the “reverse cause hypothesis,” whereby dairying was adopted in populations with preadaptive high LP allele frequencies. Analysis based on the cons…

Myeloperoxidase mediates neutrophil activation by association with CD11b/CD18 integrins.

2004

Recruitment and activation of polymorphonuclear neutrophils (PMNs) reflects a primary immunological response to invading pathogens and has also emerged as a hallmark of vascular inflammation. One of the principal enzymes released upon PMN activation is myeloperoxidase (MPO), a heme protein that not only generates cytotoxic oxidants but also impacts deleteriously on nitric oxide-dependent signaling cascades within the vasculature. Because MPO also associates with the membrane of PMN, we evaluated whether MPO could also function as an autocrine modulator of PMN activation. The extent of PMN membrane-associated MPO was elevated in patients with acute inflammatory vascular disease compared with…

Metafora e mitopoietica nella concezione musicale romantica

2009

Caracterización funcional de GTPBP3: una proteína G implicada en la modificación de tRNAs mitocondriales.

2015

Determinadas enfermedades mitocondriales, como MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes), MERRF (myoclonus epilepsy associated with ragged-red-fibers), cardiomiopatía hipertrófica y acidosis láctica dependiente de GTPBP3, cardiomiopatía hipertrófica infantil y acidosis láctica dependiente de MTO1 y fallo hepático infantil agudo dependiente de TRMU, están asociadas con una disfunción severa del sistema de fosforilación oxidativa (OXPHOS) que, se cree, podría ser resultado de defectos en la modificación postranscripcional de la uridina localizada en la posición de tambaleo (U34) de ciertos tRNAs mitocondriales (mt-tRNAs) y, por consiguiente, de u…

Tipiskākās mikroskopiskās pazīmes ar injekcijām saistītu sarkomu (ISS) gadījumā kaķiem

2017

Pētījuma būtība bija veterinārā patologa Ilzes Matīses – Van Houtanas nodrošināto kaķu ISS novērtēšana 2014. – 2016. gadā. Tika izmeklēti biopsiju paraugi, kas tika realizēta ar fiksētu un HE krāsotu preparātu izmeklēšanu un gaismas mikroskopu, un biopsijas protokolu analīze. Kopumā tika izpētīti 63 sarkomu paraugi ar kopējo griezumu skaitu - 199, kur stikliņu skaits vienam audzējam variēja no viena līdz sešiem. Vidēji no visiem triju gadu laikā izmeklētajiem audzēju paraugiem 18% sastādīja tieši ISS, un kopš 2010. gada Latvijā tiek novērota ISS incidences pieauguma tendence. Pieaugot ISS ļaundabīguma pakāpei, attiecīgi pieaug kvantitatīvie rādītāji 10 RL – daudzkodolainās šūnas, mitozes un…

Estudio del papel de los haplogrupos mitocondriales, el control glucémico y el tratamiento con metformina sobre los mecanismos moleculares subyacente…

2018

La diabetes tipo 2 (DM2) se ha convertido en un grave problema de salud a nivel mundial que está íntimamente relacionado con el desarrollo de complicaciones micro y macrovasculares. Se ha descrito que algunos haplogrupos del ADN mitocondrial (ADNmt) podrían estar involucrados en la DM2 y sus complicaciones vasculares asociadas. Por lo que nuestro primer objetivo consistió en determinar el impacto de los haplogrupos mitocondriales más frecuentes de la población española sobre las características metabólicas y los marcadores de función renal en pacientes con DM2 y controles sanos. Nuestros resultados indican que el haplogrupo JT (concretamente, el polimorfismo m.4216T> C) se asocia con un peo…

Estudio del contenido y del estado mitocondrial en el blastocisto humano

2022

El contenido de ácido desoxirribonucleico (ADN) mitocondrial (ADNmt) por célula como marcador de viabilidad embrionaria se propuso como una estrategia muy innovadora para la priorización en la selección de embriones humanos en los tratamientos reproducción asistida, siendo los embriones con menor contenido mitocondrial por célula los candidatos a ser transferidos. La hipótesis de partida se basaba en que dichos embriones presentarían un estado metabólico más apropiado y un menor estrés celular, los cuales se traducirían en una mayor probabilidad de implantación. La elevada expectación inicialmente generada alrededor de este marcador, vino acompañada de publicaciones con resultados contradic…