Search results for " Molecular Marker"

Molecular and morphological diversity of on-farm hazelnut (Corylus avellana L.) landraces from southern Europe and their role in the origin and diffu…

2013

Hazelnut (Corylus avellana L.) is a traditional nut crop in southern Europe. Germplasm exploration conducted on-farm in five countries (Portugal, Spain, Italy, Slovenia, and Greece) identified 77 landraces. The present work describes phenotypic variation in nut and husk traits and investigates genetic relationships using ten simple sequence repeat (SSR) markers among these landraces, 57 well-known references cultivars, and 19 wild accessions. Among the 77 landraces, 42 had unique fingerprints while 35 showed a SSR profile identical to a known cultivar. Among the 42 unique landraces, morphological observations revealed high phenotypic diversity, and some had characteristics appreciated by th…

Molecular approach for the characterization of ancient /degraded Cyperus sp.specimens

2013

The aim of this work has been to apply and improve the efficiency of molecular tools for the identification of Cyperus sp. from difTerent sources. Total DNA was extracted from fresh, degraded and ancient specimens. DNA molecules were the template for in vitro amplification of specific target regions referred to rcbL plastid gene (ribulose-l,5 -biphosphate carboxylase/oxygenase large subunit). Dedicated DNA extraction and amplification protocols have been applied specifically for fresh/degraded and ancient samples. Particularly, specific set of primer, annealing temperatures and reaction mixtures have been performed. The PCR products, single DNA fragments, were resolved on agarose gel electr…

The TP53 colorectal cancer international collaborative study on the prognostic and predictive significance of p53 mutation: influence of tumor site, …

2005

Purpose The aims of the TP53 Colorectal Cancer (CRC) International Collaborative Study were to evaluate the possible associations between specific TP53 mutations and tumor site, and to evaluate the prognostic and predictive significance of these mutations in different site, stage, and treatment subgroups. Patients and Methods A total of 3,583 CRC patients from 25 different research groups in 17 countries were recruited to the study. Patients were divided into three groups according to site of the primary tumor. TP53 mutational analyses spanned exons 4 to 8. Results TP53 mutations were found in 34% of the proximal colon tumors and in 45% of the distal colon and rectal tumors. They were assoc…

Genetic architecture of the marbled goby Pomatoschistus marmoratus (Perciformes, Gobiidae) in the Mediterranean Sea

2011

The marbled goby Pomatoschistus marmoratus, a species inhabiting coastal Mediterranean lagoons, has been studied by measuring its mitochondrial DNA variation. This analysis revealed a Mediterranean west vs east split and, subsequently, an eastern differentiation among the Libyan–Tunisian Gulf, the Adriatic Sea and the Aegean Sea. The high cohesion between the samples collected in the vast area of western Mediterranean contrasts with the genetic mosaic of the more sub-structured eastern Mediterranean. This western homogeneity can not yet be fully explained even if a human-mediated migratory flow, due to a maritime traffic, has been posited. The pattern in the eastern basin revealed a genetic…

A contribution to the phylogeny and biogeography of the genus Isoetes (Isoetaceae, Lycopodiidae) in the Mediterranean region

2019

To better understand the phylogeny of Isoetes species in the Mediterranean region, sequences of the second intron of a LFY-like homolog were determined for six populations of three Mediterranean Isoetes species and analyzed with other LFY and ITS sequences of Euro-Mediterranean species available in GenBank. Nomenclature of selected species was updated according to recent literature. Investigations of voucher specimens from previously sequenced taxa revealed that a specimen of I. histrix from Crete, Greece used in several previous molecular studies is probably I. phrygia. Analysis of the limited LFY and ITS DNA sequence data presented here agrees with the organization of Mediterranean specie…

Morpho-agronomic and genetic diversity among twelve sicilian agro-ecotypes of lentil (Lens culinaris)

2015

Although Sicily is relatively small (about 25000 km2), it accounts for several agro-ecotypes of lentil (Lens culinaris Medik.), for many of which no data on morphological, agronomic, and organoleptic characteristics are available to date. Thus, experiments were performed to characterize 12 lentil agro-ecotypes from different areas of Sicily, including some small islands surrounding the main island, and to assess the extent of genetic diversity (by means of six inter-simple sequence repeat [ISSR] primers). A famous agro-ecotype from central Italy (Castelluccio di Norcia) and two commercial varieties from Canada (Eston and Laird) were also included in the study. The results showed a large deg…

Development of an efficient regeneration protocol for pear rootstock Pyrodwarf and assessment of somaclonal variability using SSR analysis.

2009

Citrus biotechnology

2020

Agricultural crops that can better withstand the changing climatic and pathogen landscape have been produced through natural selection throughout the millennia and, in recent years, through the process of human-assisted plant breeding and selection. However, a lack of genetic diversity in many commercially cultivated crops (due to monoculture) has made them more vulnerable to biotic and abiotic stresses (Esquinas-Alcazar, 2005; Keneni et al., 2012). Citrus, belonging to the Rutaceae family, is one of the most important commercial woody fruit crops in the tropical and subtropical areas of the world. The origin of citrus is traced back to parts of tropical and subtropical Southeast Asia (Wu e…

Genetic Characterization of Tunisian Olive Table Cultivars (Olea europaea L.): Inventory Based on Microsatellite Analysis

2010

Olive (Olea europaea L.) is an ancient crop which spread from the Middle East towards the westem Mediterranean region. In addition lo its agronomical and economical importance, the olive industry has a great social and cultural interest as part of our heritage. In order to characterize and to study the genetic relationship among cultivars, five polymorphic microsatellite loci were used in this study and were generated a total of 56 polymorphic alleles with an average of 11 alleles by locus. For all genotypes, the highest number of polymorphic alleles was obtained by the locus UD0043 and GAPU 103. The polymorphism index content ranged from 0.836 for the locus ssrOeUADCA-04 and 0.919 for the …

Molecular characterization of the Sicilian goat populations

2014

The Sicilian goats are characterized by a strong population admixture structure, caused by geographical location of the farms, influences of natural mating and traditional breeding systems where flock represents an important breeding unit. Nowadays, several local populations are reared in Sicily, some of which do not have a recognized defined genetic structure, having taken origin from several crosses between animals of different breeds/populations sharing the same environment. The aim of this work was to characterize the Sicilian autochthonous goat populations using microsatellite markers and genetic polymorphisms of casein genes