Search results for " NGS"
showing 5 items of 25 documents
Genomic analysis and lineage identification of SARS-CoV-2 strains isolated from migrants travelling through the Libyan route
2021
Many African countries, representing the origin of the majority of refugees, asylum-seekers, and other migrants, toward regions bordering on the Mediterranean area, are experiencing sustained local transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Sicily is one of the main entry gates of migrants crossing into Europe. We conducted a pilot study, based on the full-genome sequencing of SARS-CoV-2 strains isolated from migrants coming to Sicily by crossing the Mediterranean Sea, with the aim to investigate the viral genome polymorphism and to describe their genetic variations and the phylogenetic relationships. On June 21, a nongovernmental organization vessel rescu…
Temporal dynamic of biofilms enhances the settlement of the central-1 Mediterranean reef2 builder Dendropoma cristatum (Biondi, 1859)
2021
Research on marine invertebrate settlement provides baseline knowledge for restoration technique implementation, especially for biogenic engineers with limited dispersion ability. Previously, we determined that the maturity of a biofilm strongly enhances the settlement of the vermetid reef-builder Dendropoma cristatum . In order to elucidate which biofilm features support a higher settlement of this species, we analyse the structure and composition of a marine biofilm over time, through microscopic observations, eukaryotic and prokaryotic fingerprinting analyses and 16S rDNA Illumina sequencing. The vermetid settlement temporal increase matched with the higher biofilm extent on the substrat…
Temporal dynamic of biofilms enhances the settlement of the central-Mediterranean reef-builder Dendropoma cristatum (Biondi, 1859).
2021
Abstract Research on marine invertebrate settlement provides baseline knowledge for restoration technique implementation, especially for biogenic engineers with limited dispersion ability. Previously, we determined that the maturity of a biofilm strongly enhances the settlement of the vermetid reef-builder Dendropoma cristatum. To elucidate settlement-related biofilm features, here we analyse the structure and composition of marine biofilms over time, through microscopic observations, eukaryotic and prokaryotic fingerprinting analyses and 16S rDNA Illumina sequencing. The vermetid settlement temporal increase matched with the higher biofilm coverage on the substratum and the reduction of th…
Ārpusšūnu cirkulējošo nukleīnskābju izpēte hipofīzes adenomas pacientos
2019
Līdz šim literatūrā nav ziņojumu par ccfDNA un cirkulējošo miRNS izpēti HA pacientu plazmā. Darbā tika novērtēta iespējamība detektēt HA izcelsmes ccfDNA plazmā, veicot mērķētu NGS pozīcijās, kuras saturēja HA somatiskās mutācijas, un veikts pētījums par miRNS sastāva izmaiņu detektēšanu plazmā 24 stundas pēc HA rezekcijas. HA eksomos atklātās somatiskās mutācijas tikai detektētas septiņās no 16 pārbaudītajām pozīcijām ccfDNA amplikonos. Divās ccfDNA pozīcijās mutantā alēle bija vairāk nekā 2% no kopējā nolasījumu skaita, kas varētu liecināt par šo ccfDNA izcelsmi no HA. Piecās pozīcijās no diviem HA pacientiem mutantās alēles īpatsvars ccfDNA bija ap 50%, kaut gan šīs mutācijas netika kons…
The seed endophytic microbiota of Citrus limon L. Burm. F
2017
Background Plant seeds possess a complex microbiota which may play a crucial rolein a plethora of mechanisms, such as preservation, germination, seedling development, plant growth and health. In particular, seed endophytic microbiota is gaining more and more consideration due to the fact that it may be vertically transmitted to ensure individual competitive advantages. The Citrusspecies, including C.limon,areone of the most economically important evergreen fruit crops in the world. Objectives i)Identification and characterization ofmicrobial isolates from Citrus limon L. Burm. F. seed endosphere. ii) Structure of Citrus limon L. Burm. F. seed endosphytic microbiota. Methods -Surface-sterili…