Search results for " Needle"

showing 10 items of 115 documents

Circulating microparticles as disease-specific biomarkers of severity of inflammation in patients with hepatitis C or nonalcoholic steatohepatitis.

2012

Background & Aims Microparticles released into the bloodstream upon activation or apoptosis of CD4+ and CD8+ T cells correlate with inflammation as determined by histologic analysis in patients with chronic hepatitis C (CHC). Patients with nonalcoholic fatty liver (NAFL) or nonalcoholic steatohepatitis (NASH) can be differentiated from those with CHC based on activation of distinct sets of immune cells in the liver. Methods We compared profiles of circulating microparticles from patients with NAFL and NASH (n = 67) to those of CHC (n = 42), with healthy individuals (controls) using flow cytometry; the profiles were correlated with inflammation grade and fibrosis stage based on histologic an…

AdultCD4-Positive T-LymphocytesLiver CirrhosisMaleLymphocyteBiologyCD8-Positive T-LymphocytesChronic liver diseaseSeverity of Illness IndexArticleCell-Derived MicroparticlesDiagnosis DifferentialImmune systemFibrosisCell-Derived MicroparticlesNon-alcoholic Fatty Liver DiseasemedicineHumansAgedAged 80 and overInflammationHepatologyFatty liverBiopsy NeedleGastroenterologyAlanine TransaminaseHepatitis CHepatitis C ChronicMiddle Agedmedicine.diseaseFlow CytometryFatty Livermedicine.anatomical_structureAlanine transaminaseLiverROC CurveCase-Control StudiesImmunologybiology.proteinLinear ModelsFemaleBiomarkersGastroenterology
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Determination of oestrogen receptors with monoclonal antibodies in fine needle aspirates of breast carcinoma.

1989

Fifty patients with operable breast carcinoma underwent fine needle aspiration for cytological examination. The smears were prepared by means of the immunocytochemical method using monoclonal antibodies for the determination of the oestrogen receptors (ER). After surgery the contents of the ER were determined with the traditional biochemical technique. The results of the immunocytochemical method showed 31 positives, two of which disagreed with the biochemical results, 15 negatives and four cases which could not be assessed due to the absence of adequate numbers of cells. The ICA staining for ER was expressed on a semiquantitative basis; there was a significant correlation between this and …

AdultCancer ResearchPathologymedicine.medical_specialtymedicine.drug_classBreast NeoplasmsBiologyMonoclonal antibodyBiopsyCarcinomamedicineHumansAgedmedicine.diagnostic_testEpitheliomaBiopsy NeedleAntibodies MonoclonalMiddle Agedmedicine.diseaseStainingFine-needle aspirationOncologyReceptors EstrogenMonoclonalFemaleBreast carcinomaResearch ArticleBritish journal of cancer
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Axillary Nodal Burden in Breast Cancer Patients With Pre-operative Fine Needle Aspiration-proven Positive Lymph Nodes Compared to Those With Positive…

2019

BACKGROUND/AIM: Recent years have seen a considerable shift to a more conservative management of the axilla in patients with positive axillary sentinel lymph nodes. The aim of this study was to determine whether some breast cancer patients with a preoperative ultrasound-guided needle aspiration biopsy proven positive node could potentially be spared an axillary lymph node dissection according to the ACOSOG Z0011 trial criteria. PATIENTS AND METHODS: A retrospective review was performed involving 623 breast cancer patients who underwent axillary lymph node dissection after either ultrasound-guided needle aspiration biopsy proven positive node or sentinel lymph node biopsy. RESULTS: Patients …

AdultCancer Researchmedicine.medical_specialtyaxillary nodal burdenCytodiagnosisSentinel lymph nodeBiopsy Fine-NeedleBreast NeoplasmsSensitivity and SpecificityGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciences0302 clinical medicineBreast cancerBreast cancerBiopsyPreoperative CareMedicineHumansBreastsentinel lymph node biopsyEndoscopic Ultrasound-Guided Fine Needle AspirationAgedRetrospective StudiesPharmacologyAged 80 and overmedicine.diagnostic_testbusiness.industryAxillary Lymph Node DissectionReproducibility of ResultsMiddle Agedmedicine.diseaseAxillaFine-needle aspirationmedicine.anatomical_structure030220 oncology & carcinogenesisLymphatic MetastasisAxillaFemaleRadiologyLymphLymph NodesbusinessNODALResearch Article
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The extent of HLA-DR expression on HLA-DR+Tregs allows the identification of patients with clinically relevant borderline rejection

2013

Regulatory T cells (Tregs) were shown to be involved into the pathogenesis of acute rejection after transplantation. The suppressive activity of the total regulatory T cell pool depends on its percentage of highly suppressive HLA-DR(+) -Treg cells. Therefore, both the suppressive activity of the total Treg pool and the extent of HLA-DR expression of HLA-DR(+) -Tregs (MFI HLA-DR) were estimated in non transplanted volunteers, patients with end-stage renal failure (ESRF), healthy renal transplant patients with suspicion on rejection, due to sole histological Bord-R or sole acute renal failure (ARF), and patients with clinically relevant borderline rejection (Bord-R and ARF). Compared to patie…

AdultGraft RejectionMaleRegulatory T cellRisk AssessmentSensitivity and SpecificityT-Lymphocytes RegulatoryFlow cytometryCohort StudiesPathogenesisYoung AdultPredictive Value of TestsReference ValuesBiopsymedicineHLA-DRHumansSurvival rateAgedSubclinical infectionTransplantationmedicine.diagnostic_testbusiness.industryBiopsy NeedleForkhead Transcription FactorsHLA-DR AntigensMiddle AgedFlow CytometryImmunohistochemistryKidney TransplantationSurvival RateTransplantationTreatment Outcomemedicine.anatomical_structureROC CurveCase-Control StudiesImmunologyLinear ModelsKidney Failure ChronicFemalebusinessBiomarkersTransplant International
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The obsolescent renal glomerulus ? Collapse, sclerosis, hyalinosis, fibrosis

1977

By light and electron microscopical examination it is shown that four structural components can contribute to obsolescent glomeruli: capillary basement membranes, enriched mesangium matrix, “vascular” hyalin and collagen fibers. Each of these components can bring about glomerular damage alone. One non-reactive form — a glomerular collapse with only basement membrane remnants — can be separated from three reactive forms: the accumulation of mesangium matrix (sclerosis or matrix-sclerosis), deposition of vascular hyalin (hyalinosis in the narrow sense), and fiber development within the former urinary space (fibrosis or fibro-sclerosis). The use of the term “fibrinoid” in place of the descript…

AdultHyalinPathologymedicine.medical_specialtyHistologyRenal glomerulusKidney GlomerulusBasement MembranePathology and Forensic MedicineFibrosismedicineHumansChildMolecular BiologyCollapse (medical)HyalineBasement membraneMesangium matrixSclerosisUrinary spaceGlomerulosclerosis Focal SegmentalChemistryBiopsy NeedleGlomerulosclerosisCell BiologyGeneral MedicineAnatomymedicine.diseaseMicroscopy Electronmedicine.anatomical_structureCollagenAnatomymedicine.symptomVirchows Archiv A Pathological Anatomy and Histology
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Metabolite identification in human liver needle biopsies by high-resolution magic angle spinning1H NMR spectroscopy

2006

High-resolution magic angle spinning (HR-MAS) 1 H NMR spectroscopy of intact human liver needle biopsies has not been previously reported. HR-MAS NMR spectra collected on 17 specimens with tissue amounts between � 0.5 and 12 mg showed very good spectral resolution and signal-to-noise ratios. One-dimensional 1 H spectra revealed many intense signals corresponding to cellular metabolites. In addition, some high molecular weight metabolites, such as glycogen and mobile fatty acids, could be observed in some spectra. Resonance assignments for 22 metabolites were obtained by combining the analysis of three different types of 1D 1 H spectral editing, such as T2 filtering or the nuclear Overhauser…

AdultLiver CirrhosisMaleMagnetic Resonance SpectroscopyMetaboliteNuclear Overhauser effectSensitivity and Specificitychemistry.chemical_compoundNuclear magnetic resonanceMagic angle spinningHumansRadiology Nuclear Medicine and imagingSpectroscopySpectroscopyAgedHepatitis ChronicChemistryBiopsy NeedleReproducibility of ResultsResonanceNuclear magnetic resonance spectroscopyMiddle AgedNMR spectra databaseProton NMRMolecular MedicineFemaleSpin Labelslipids (amino acids peptides and proteins)ProtonsBiomarkersNMR in Biomedicine
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Long-term follow-up of hepatitis C virus-positive patients with persistently normal serum transaminases

2013

Material and methods. This study prospectively evaluated the progression of liver disease in a group of anti-HCV-positive patients with persistently normal ALT levels (PNALT) who were HCV-RNA positive. Patients selected for this study were those who presented with PNALT according to the Italian Association for the Study of the Liver (AISF) criteria in the year 1995/96 and underwent liver biopsy. They were divided into two groups according to their ALT evolution. Forty-five patients were included in this study. Results. After a median follow-up time of 180 months twenty-five of them maintained PNALT, but two of these developed liver cirrhosis (LC) in a mean time of 174 and 202 months, respec…

AdultLiver CirrhosisMalemedicine.medical_specialtyCirrhosisLong term follow upSpecialties of internal medicineNormal serumChronic hepatitis CGastroenterologyAntiviral AgentsBody Mass IndexLiver diseaseInterferon treatmentInternal medicinemedicineHumansLongitudinal StudiesProspective StudiesUltrasonography Doppler ColorChronic hepatitis C. Persistently normal transaminases. Liver histology. Progression of disease. Interferon treatment.Proportional Hazards ModelsHepatologymedicine.diagnostic_testbusiness.industrySignificant differenceHistologyAlanine TransaminaseGeneral MedicineHepatitis C AntibodiesHepatitis C ChronicMiddle Agedmedicine.diseaseSurgeryHepatitis C Virus PositiveLiverRC581-951Liver biopsyDisease ProgressionElasticity Imaging TechniquesRNA ViralFemaleBiopsy Large-Core NeedlePersistently normal transaminasesbusinessLiver histologyProgression of disease
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Metastatic Anterior Chamber Non-Hodgkin Lymphoma in a Patient With Acquired Immunodeficiency Syndrome

1997

Purpose To alert ophthalmologists to the possibility of metastatic anterior chamber intraocular non-Hodgkin lymphoma in patients with acquired immunodeficiency syndrome (AIDS). Methods We examined a 19-year-old man with AIDS who had an anterior uveitis with pseudohypopyon and a history of 2 months of malaise. A specimen of the lesion was obtained with a 25-gauge needle for pathologic examination. RESULTS: Systemic exploration showed hepatomegaly. Abdominal computed tomography showed multiple lesions in the liver and spleen. Fine-needle aspiration disclosed a high-grade non-Hodgkin lymphoma. The result of a fine-needle aspiration obtained from a lesion in the anterior chamber was consistent …

AdultMaleAcquired Immunodeficiency SyndromePathologymedicine.medical_specialtyAnterior Chamberbusiness.industryEye NeoplasmsLymphoma Non-HodgkinEye diseaseBiopsy Needlemedicine.diseaseLymphomaMetastasisMalaiseLesionOphthalmologyImmunopathologymedicineHumansmedicine.symptombusinessComplicationUveitisAmerican Journal of Ophthalmology
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Ultrasound-guided fine-needle aspiration cytology in the early detection of melanoma metastases.

2000

BACKGROUND. The early detection and treatment of tumor recurrences in melanoma patients is dependent on reliable, sensitive, and specific techniques to verify suspected tumor metastases. As of now, fine-needle aspiration cytology (FNAC) has yet to establish itself in the routine follow-up of melanoma patients. METHODS. FNAC procedures were performed in melanoma patients with palpable tumors or nonpalpable, ultrasonically suspicious lesions. Cytodiagnostic evaluation of fine-needle samples obtained from suspicious lesions was performed morphologically. Findings were validated either by histopathologic diagnosis or prolonged clinical follow-up. RESULTS. The cytologic examination of 739 FNACs …

AdultMaleCancer Researchmedicine.medical_specialtySkin NeoplasmsAdolescentEarly detectionSensitivity and SpecificityMetastasisFine needle aspiration cytologyCytologyBiopsymedicineHumansSampling (medicine)Neoplasm MetastasisMelanomaUltrasonography InterventionalAgedNeoplasm Stagingmedicine.diagnostic_testbusiness.industryMelanomaUltrasoundBiopsy NeedleReproducibility of ResultsMiddle Agedmedicine.diseasebody regionsOncologyFemaleRadiologybusinessCancer
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Circumscribed palmar or plantar hypokeratosis: a distinctive epidermal malformation of the palms or soles.

2002

Abstract Background: Epidermal malformations of the skin include a group of heterogeneous developmental defects that result from errors in morphogenesis of the epidermis during intrauterine life. Objective: The purpose of this study was to report the clinical and histopathologic features of a distinctive epidermal malformation involving the skin of the palms or soles. Methods: Ten patients were included in this study. All of them showed the same clinical features that consisted of a solitary circumscribed and circular area of erythematous depressed skin on the palm or on the sole. Diagnosis was confirmed by histopathologic study. Results: All patients were middle aged or elderly. Nine patie…

AdultMalePathologymedicine.medical_specialtyKeratosisBowen's DiseaseDermatologySeverity of Illness IndexSampling StudiesLesionDiagnosis DifferentialKeratoderma PalmoplantarmedicineHumansProspective StudiesKeratodermaAgedBowen's diseaseCorneocyteintegumentary systembusiness.industryBiopsy NeedleAnatomyMiddle Agedmedicine.diseaseImmunohistochemistryPorokeratosismedicine.anatomical_structureFemaleEpidermismedicine.symptomDifferential diagnosisEpidermisbusinessPorokeratosisJournal of the American Academy of Dermatology
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