Search results for " Northern"
showing 10 items of 216 documents
Molecular cloning and characterization of a Candida albicans gene coding for cytochrome c haem lyase and a cell wall-related protein.
1998
Immunoscreening of a Candida albicans cDNA library with a monoclonal antibody (mAb 4C12) recognizing an epitope present in high-molecular-weight mannoprotein (HMWM) components specific for the mycelial cell walls (a 180 kDa component and a polydispersed 260 kDa species) resulted in the isolation of the gene CaCYC3 encoding for cytochrome c haem lyase (CCHL). The CaCYC3 gene was transcribed preferentially in mycelial cells in which two mRNA transcripts of 0.8 and 1 kb were found. The nucleotide and the deduced amino acid sequences of this gene displayed 45% homology and 46% identity, respectively, to the Saccharomyces cerevisiae CYC3 gene and shared common features with other reported genes …
Characterization of aging-associated up-regulation of constitutive nuclear factor-kappa B binding activity.
2001
Changes occur in gene expression during aging in vivo and in replicative senescence in vitro, suggesting that aging can affect gene regulation. We have recently observed age-related changes in ubiquitously expressed, oxidative stress-responsive nuclear factor-kappa B (NF-kappa B) pathway during aging. Here we report a significant age-related increase in nuclear NF-kappa B binding activity together with increased protein levels of p52 and p65 components in rat liver. An additional, higher molecular weight protein band seen in their western blots suggests that their post-translational modification (but not phosphorylation) occurs in liver, which might affect their nuclear localization and bin…
Isolation, sequence analysis and characterization of cDNA clones coding for the C chain of mouse C1q. Sequence similarity of complement subcomponent …
1992
A mouse macrophage lambda gt11 cDNA library was screened using a genomic DNA clone coding for the C-chain gene of human C1q. Approximately 600,000 recombinant phage plaques were hybridized with peroxidase-labeled human C-chain probe and detected by enhanced chemiluminescence. Five positive clones were obtained. The size of the full-length cDNA is 1019 bp. The sequence identity of the nucleotide sequence with human C1q C chain is 79%, the identity of the deduced amino acid sequences is 73%. The mouse C1q C chain exhibits the same structural features as the human C chain, e.g. conservation of the cysteine residues. Like the mouse A chain, the mouse C chain has an RGD sequence that may be reco…
Genetic organization of the mle locus and identification of a mleR-like gene from Leuconostoc oenos
1996
Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension analysis showed that the mle operon has a single transcription start site located 17 nucleotides upstream of the ATG translation start site for the mleA gene. We found sequences, TTGACT and TATGAT (which are separated by 18 bp), that are closely related to the gram-positive and Escherichia coli consensus promoter sequences. Upstream of the mleA gene, an 894-bp open reading frame t…
Algal assemblages response to fish-farm biodeposition in a Southern Mediterranean area (Gulf of Castellammare, Sicily): preliminary data
2005
This study, carried out in the Gulf of Castellammare (northern coast of Sicily), concerns the effects of fish-farm nutrient enrichment on the algal assemblage growing on artificial substrata of cages. A total of 51 taxa were identified (3 Cyanophyceae, 3 Phaeophyceae, 5 Bacillariophyceae, 3 Chlorophyceae, 6 Ulvophyceae and 31 Rhodophyceae). A dominance of opportunistic species was highlighted, following a gradient from impact areas to controls.
VARIABILITY OF DEPOSITIONAL SETTING ALONG THE NORTH-WESTERN SICILY CONTINENTAL SHELF (ITALY) DURING LATE QUATERNARY: EFFECTS OF SEA LEVEL CHANGES AND…
2012
The geological, geomorphological and sedimentological features of the north-western Sicily continental shelf are here illustrated with the aim to propose a geological model able to explain the Neogene-Quaternary evolution of the Sicilian continental margin in the context of the central Mediterranean region. Above the continental shelf and upper slope the sedimentary succession, showing along the different sectors of the margin considerably variable internal geometry and stratigraphic relationships with the underlying units, is interpreted as a IV order depositional sequence (Late Quaternary Depositional Sequence, LQDS) deposited during the last eustatic change (last 125 ky). The lower bound…
Contatti culturali, identità e popolamento nel territorio imerese
2016
Il lavoro prende in considerazione il problema dei rapporti tra coloni greci e comunità indigene nel territorio di Himera, sulla base dei risultati della prospezione archeologica condotta per tre decenni nelle valli dei fiumi Imera settentrionale, Torto e S. Leonardo. Vengono esaminati gli elementi di cultura materiale che permettono di definire i modi dell'interazione culturale tra coloni e indigeni, nonché di ricostruire i paesaggi politici, socio-economici e il paesaggio del sacro nelle diverse aree indagate e nei diversi comprensori. La ricerca infatti permette di delineare, oltre alle usuali organizzazioni insediative e agricole della chora politiké e della éremos chora, anche articola…
A syntactic analysis of the subject clitic a in the Friulian variety of Campone
2015
This article presents a syntactic analysis of the third person subject clitic a in Camponese, a heretofore unstudied Friulian variety. Following Poletto's (2000) map of subject clitics, we argue that it bears [+third person] features, and is, in fact, the spell-out of the functional head Subj°, located in the highest projection of TP (following Rizzi & Shlonsky 2007). In the first part of the article, we offer a detailed description of the distribution and syntactic properties of the subject clitic a, identifying its position in relation to the other elements that occur in the CP and TP. In the second part we discuss two proposals put forward to account for split clitics like a-l in the…
dfh is a Drosophila homolog of the Friedreich's ataxia disease gene
2000
Abstract A putative Drosophila homolog of the Friedreich's ataxia disease gene (FRDA) has been cloned and characterized; it has been named Drosophila frataxin homolog (dfh). It is located at 8C/D position on X chromosome and is spread over 1 kb, a much smaller genomic region than the human gene. Its genomic organization is simple, with a single intron dividing the coding region into two exons. The predicted encoded product has 190 amino acids, being considered a frataxin-like protein on the basis of the sequence and secondary structure conservation when compared with human frataxin and related proteins from other eukaryotes. The closest match between the Drosophila and the human proteins in…
Complete sequence, expression and evolution of two members of the hexamerin protein family during the larval development of the rice moth, Corcyra ce…
2002
Three distinct types of storage hexamerins are expressed in the "last-instar" larvae of the rice moth, Corcyra cephalonica. A cDNA expression library was constructed from fat body-RNA and screened with a polyclonal antibody raised against purified hexamerin (SP2) of Corcyra cephalonica. Two slightly different "full-length" hexamerin cDNA clones (Hex2a and Hex2b) were isolated and sequenced. Both include open reading frames of 2109 bp which are translated into polypeptides of 703 amino acids with 92.5% identity. Signal peptides of 19 amino acids are present at the N-termini. The 684 amino acids native proteins have a high content of aryl groups (17.6%). According to both the criteria for ami…