Search results for " Positive"
showing 10 items of 397 documents
Validation of a novel automatic deposition of bacteria and yeasts on MALDI target for MALDI-TOF MS-based identification using MALDI Colonyst robot
2017
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) -based identification of bacteria and fungi significantly changed the diagnostic process in clinical microbiology. We describe here a novel technique for bacterial and yeast deposition on MALDI target using an automated workflow resulting in an increase of the microbes' score of MALDI identification. We also provide a comparison of four different sample preparation methods. In the first step of the study, 100 Gram-negative bacteria, 100 Gram-positive bacteria, 20 anaerobic bacteria and 20 yeasts were spotted on the MALDI target using manual deposition, semi-extraction, wet deposition onto 70% formic …
The Added-Value of Remotely-Sensed Soil Moisture Data for Agricultural Drought Detection in Argentina
2021
In countries where the economy relies mostly on agricultural-livestock activities, such as Argentina, droughts cause significant economic losses. Currently, the most-used drought indices by the Argentinian National Meteorological and Hydrological Services are based on field precipitation data, such as the standardized precipitation index (SPI) and the standardized precipitation evapotranspiration index (SPEI). In this article, we explored the performance of the satellite-based soil moisture agricultural drought index (SMADI) for agricultural drought detection in Argentina during 2010-2015, and compared it with the one from the standardized soil moisture anomalies (SSMA), SPI and SPEI (at on…
Sleep Apnea, Sleepiness, and Driving Risk
2019
Obstructive sleep apnea is associated with excessive daytime sleepiness in about 50% of cases, and with increased risk of driving accidents. Treatment with continuous positive airway pressure effectively decreases such risk, but compliance with continuous positive airway pressure treatment is often suboptimal. According to the European Union Directive on driving risk, retention of a driving license in patients with obstructive sleep apnea requires assessment of sleepiness and adherence to continuous positive airway pressure treatment, but there remains uncertainty on the optimal methods to assess sleepiness on a large scale.
Affirmative action in India: redistribuzione di beni e creazione di identità
2009
L’India è probabilmente lo stato in cui l’affirmative action riguarda la più ampia percentuale di popolazione e i più svariati ambiti della vita pubblica; inoltre le prime forme di agevolazione di gruppi svantaggiati previste dal governo risalgono ad oltre 130 anni fa. La reservation policy (il nome con cui in India vengono chiamate le azioni positive) è argomento ricorrente del dibattito pubblico: tema eminente di ogni programma elettorale, influisce fortemente nella costituzione della base dei partiti e nella scelta dei rappresentanti, è contestata o invocata in molte battaglie politiche e in numerosi conflitti sociali che agitano il paese. Ha contribuito fortemente a profilare le identit…
Foster Carbon-Neutrality in the Built Environment: A Blockchain-Based Approach for the Energy Interaction Among Buildings
2022
Decarbonizing urban environments and reducing their energy consumption is one of modern society biggest commitments. In this regard, both the scientific community and international governments have been giving special consideration on improving the energy-environmental performance of mostly single buildings; recently the focus has been shifting towards a broader Positive Energy District (PED)/ Energy Community (EC) vision, from small clustered group of buildings to neighborhoods up until urban settlements. In this respect, integrating the use of renewable energy resources (RES) and actively involving building users can have a significant environmental impact on local areas. Within this fram…
Well-being and tourist experiences : a state of the art review of positive psychology studies
2017
Currently, well-being concerns individuals but also businesses and even countries. This article thus provides a synthesis of psychological researches on well-being and of those bringing closer well-being and tourism, this activity maintaining obvious links with well-being. The aim is to better understand their relationships and to offer, in particular to tourist operators, marketing recommendations suggested by the reviewed studies.
An initial comparative map of copy number variations in the goat (Capra hircus) genome
2010
Abstract Background The goat (Capra hircus) represents one of the most important farm animal species. It is reared in all continents with an estimated world population of about 800 million of animals. Despite its importance, studies on the goat genome are still in their infancy compared to those in other farm animal species. Comparative mapping between cattle and goat showed only a few rearrangements in agreement with the similarity of chromosome banding. We carried out a cross species cattle-goat array comparative genome hybridization (aCGH) experiment in order to identify copy number variations (CNVs) in the goat genome analysing animals of different breeds (Saanen, Camosciata delle Alpi,…
Role of exosomes released by chronic myelogenous leukemia cells in angiogenesis
2012
The present study is designed to assess if exosomes released from Chronic Myelogenous Leukemia (CML) cells may modulate angiogenesis. We have isolated and characterized the exosomes generated from LAMA84 CML cells and demonstrated that addition of exosomes to human vascular endothelial cells (HUVEC) induces an increase of both ICAM-1 and VCAM-1 cell adhesion molecules and interleukin-8 expression. The stimulation of cell-cell adhesion molecules was paralleled by a dose-dependent increase of adhesion of CML cells to a HUVEC monolayer. We further showed that the treatment with exosomes from CML cells caused an increase in endothelial cell motility accompanied by a loss of VE-cadherin and β-ca…
Novel pathway in Bcr-Abl signal transduction involves Akt-independent, PLC-γ1-driven activation of mTOR/p70S6-kinase pathway
2009
In chronic myeloid leukemia, activation of the phosphoinositide 3-kinase (PI3K)/Akt pathway is crucial for survival and proliferation of leukemic cells. Essential downstream molecules involve mammalian target of rapamycin (mTOR) and S6-kinase. Here, we present a comprehensive analysis of the molecular events involved in activation of these key signaling pathways. We provide evidence for a previously unrecognized phospholipase C-gamma1 (PLC-gamma1)-controlled mechanism of mTOR/p70S6-kinase activation, which operates in parallel to the classical Akt-dependent machinery. Short-term imatinib treatment of Bcr-Abl-positive cells caused dephosphorylation of p70S6-K and S6-protein without inactivat…
Exosomal shuttling of miR-126 in endothelial cells modulates adhesive and migratory abilities of chronic myelogenous leukemia cells.
2014
BACKGROUND: Recent findings indicate that exosomes released from cancer cells contain microRNAs (miRNAs) that may be delivered to cells of tumor microenvironment. RESULTS: To elucidate whether miRNAs secreted from chronic myelogenous leukemia cells (CML) are shuttled into endothelial cells thus affecting their phenotype, we first analysed miRNAs content in LAMA84 exosomes. Among the 124 miRNAs identified in LAMA84 exosomes, we focused our attention on miR-126 which was found to be over-overexpressed in exosomes compared with producing parental cells. Transfection of LAMA84 with Cy3-labelled miR-126 and co-culture of leukemia cells with endothelial cells (EC) confirmed that miR-126 is shuttl…