Search results for " Reference"

Expression of selected molecular factors in two types of endometrial cancer

2021

BACKGROUND Endometrial cancers (EC) are a heterogeneous group of malignant neoplasms differing in etiology, clinical-pathological features and prognosis. OBJECTIVES To determine the differences between the expression of selected molecular factors and find connections between them in order to isolate possible biomarkers influencing treatment options. MATERIAL AND METHODS The investigated data involved archival histological preparations obtained from uterine EC samples taken from 137 patients, treated surgically between 2007 and 2014. The immunohistochemical Dako EnVisionTM Flex+ method was applied. RESULTS The expression of ERβ, MLH1 and BRCA1 was lower in ECI than in ECII patients. The ERα …

Total content of As, Sb, Se, Te and Bi in Spanish vegetables, cereals and pulses and estimation of the contribution of these foods to the Mediterrane…

2010

Abstract As, Sb, Se, Te and Bi were quantified in vegetables, pulses and cereals, in order to increase the available information on the presence of these elements in the Mediterranean daily intake. Samples were dry ashed and the ashes dissolved with diluted HCl. Hydride generation atomic fluorescence spectrometry was used for quantification. Limits of detection (LOD) ranged from 0.2 to 1.7 ng g−1 and the accuracy was assessed by the analysis of three certified reference materials, Rice Flour (NIST 1568a), Tomato Leaves (NIST 1573) and Cabbage (IAEA 359). Good concordance between results found and certified values were always observed. As, Sb, Se, Te and Bi levels varied in samples analysed …

Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays.

2014

ABSTRACT The molecular diagnosis of toxoplasmosis essentially relies upon laboratory-developed methods and suffers from lack of standardization, hence the large diversity of performances between laboratories. Moreover, quantifications of parasitic loads differ among centers, a fact which prevents the possible prediction of the severity of this disease as a function of parasitic loads. The objectives of this multicentric study performed in eight proficient laboratories of the Molecular Biology Pole of the French National Reference Center for Toxoplasmosis (NRC-T) were (i) to assess the suitability of a lyophilized preparation of Toxoplasma gondii as a common standard for use in this PCR-base…

Is there a widespread clone of Serratia marcescens producing outbreaks worldwide?

2021

[Background]: Serratia marcescens frequently causes outbreaks in healthcare settings. There are few studies using high-throughput sequencing (HTS) that analyse S. marcescens outbreaks. We present the analysis of two outbreaks in neonatal intensive care units (NICUs) in hospitals from the Comunitat Valenciana (CV, Spain) and the impact of using different reference genomes.

Mineral profile of Spanish commercial baby food.

2015

Commercial baby foods are an important part of the daily intake of babies from 6 to 12 months. The mineral profile of commercial baby foods in Spain was determined to establish levels of essential and non-essential elements, and their contribution to adequate intake (AI) and estimated average requirement (EAR). Thirty-five jars of commercial foods containing meat, fish, vegetables and fruit were obtained from the Spanish market and the mineral composition determined for 14 elements. In general, the baby foods analysed were sufficient for an adequate mineral intake, but contributions to AI and EAR for iron, zinc and calcium were very low (5-20%, 10-60% and 10-70%, respectively). This deficie…

2018

AbstractKnowledge of the Earth’s changing rotation is fundamental to positioning objects in space and on the planet. Nowadays, the Earth’s orientation in space is expressed by five Earth Orientation Parameters (EOP). Many applications in astronomy, geosciences, and space missions require accurate EOP predictions. Operational predictions are released daily by the Rapid Service/Prediction Centre of the International Earth Rotation and Reference Systems Service (IERS). The prediction procedures and performances differ for the three EOP classes: polar motion, rotation angle (UT1-UTC), and the two celestial pole offsets (CPO), dX and dY. The IERS Annual Report 2016 shows Rapid Service CPO predic…

Inertial modes in stratified rotating neutron stars : An evolutionary description

2005

With (non-barotropic) equations of state valid even when the neutron, proton and electron content of neutron star cores is not in beta equilibrium, we study inertial and composition gravity modes of relativistic rotating neutron stars. We solve the relativistic Euler equations in the time domain with a three dimensional numerical code based on spectral methods, in the slow rotation, relativistic Cowling and anelastic approximations. Principally, after a short description of the gravity modes due to smooth composition gradients, we focus our analysis on the question of how the inertial modes are affected by non-barotropicity of the nuclear matter. In our study, the deviation with respect to …

Seawater carbonate chemistry and nest guarding behaviour of a temperate wrasse

2021

Organisms may respond to changing environmental conditions by adjusting their behaviour (i.e., behavioural plasticity). Ocean acidification (OA), resulting from anthropogenic emissions of carbon dioxide (CO2), is predicted to impair sensory function and behaviour of fish. However, reproductive behaviours, and parental care in particular, and their role in mediating responses to OA are presently overlooked. Here, we assessed whether the nesting male ocellated wrasse Symphodus ocellatus from sites with different CO2 concentrations showed different behaviours during their breeding season. We also investigated potential re-allocation of the time-budget towards different behavioural activities b…

Seawater carbonate chemistry and shell mineralogy, microstructure, and mechanical strength of four Mediterranean gastropod species near a CO2 seep

2017

Marine CO2 seeps allow the study of the long-term effects of elevated pCO2 (ocean acidification) on marine invertebrate biomineralization. We investigated the effects of ocean acidification on shell composition and structure in four ecologically important species of Mediterranean gastropods (two limpets, a top-shell snail, and a whelk). Individuals were sampled from three sites near a volcanic CO2 seep off Vulcano Island, Italy. The three sites represented ambient (8.15 pH), moderate (8.03 pH) and low (7.73 pH) seawater mean pH. Shell mineralogy, microstructure, and mechanical strength were examined in all four species. We found that the calcite/aragonite ratio could vary and increased sign…

Insights fromsodium into the impacts of elevated pCO2 and temperature on bivalve shell formation

2017

Ocean acidification and warming are predicted to affect the ability of marine bivalves to build their shells, but little is known about the underlying mechanisms. Shell formation is an extremely complex process requiring a detailed understanding of biomineralization processes. Sodium incorporation into the shells would increase if bivalves rely on the exchange of Na+/H+ to maintain homeostasis for shell formation, thereby shedding new light on the acid-base and ionic regulation at the calcifying front. Here, we investigated the combined effects of seawater pH (8.1, 7.7 and 7.4) and temperature (16 and 22 °C) on the growth and sodium composition of the shells of the blue mussel, Mytilus edul…