Search results for " SCAFFOLD"

Three-layered porous device in PCL/PEG blend for interface tissue engineering

2016

Tissue interfaces, such as cartilage-to-bone, exhibit anisotropic structural properties, which gradually vary from one tissue to another. Consequently a regenerative scaffold designed for interface tissues should exhibit a gradient in composition, structure and mechanical features, mimicking those of the native zones. In particular, the architecture of pores plays a central role. Indeed, a biomedical implant should be designed with porosityand pore size gradients simulating the structure of the two interface tissues. One of the most common techniques to prepare porous scaffolds is the particulate leaching method, which involves the selective leaching of a mineral or organic compound as poro…

Micro – Meso scale based biomechanical models to assist the design and fabrication of scaffolds for Cardiac Tissue Regeneration

2011

Questo lavoro presenta una strategia di modellazione strutturale basata su dati di micro-architettura finalizzata a: (A) guidare fabbricazione e progetto di scaffold per ingegneria di tessuto, (B) studiare la risposta cellulare meccanica alle deformazioni strutturali. Applicazioni cliniche ideali per la metodologia proposta sono le valvole cardiache e il patch di miocardio. Custom software di analisi d’immagine e’ stato utilizzato per caratterizzare la morfologia dei campioni. Le topologie dei materiali individuate sono state impiegate per generare equivalenti modelli a rete. Il comportamento meccanico alla macro scala e’ stato studiato mediante prove di carico biassiale. Questa messe speri…

A Method to Extract the Complete Fiber Network Topology of Planar Fibrous Tissues and Scaffolds.

2010

Improving fabrication protocols and design strategies, investigating on how scaffold architecture affects cell morphology, metabolism and phenotypic expression, predicting mechanical behaviors, have increasingly become crucial goals in the development of engineered tissue scaffolds. In the present study, an image-based analysis approach that provides an automatic tool to fully characterize engineered tissues fiber network topology was developed. The following micro architectural features are detected: fiber angle distribution, fiber connectivity, fiber overlap spatial density, fiber diameter. In order to demonstrate the potential of this approach Electrospun poly(ester urethane)urea (ES-PEU…

Sterilization of three-dimensional tissue engineering scaffolds by supercritical carbon dioxide

2014

Medical devices, implants or patient-care equipment that will come into intimate contact with a patient must be effectively decontaminated to prevent infection or disease transmission. Non-sterile devices have significant ramifications for patient morbidity and mortality and two processes must be undertaken in order to make an item acceptable for use or implantation: cleaning and disinfection (or sterilization) [1]. Sterilization is the destruction of living organisms, and must be done without damaging the material surface and without compromising the bulk material strength or biocompatibility of implantable device. Common sterilization processes include steam autoclaving, gamma irradiation…

Supercritical carbon dioxide induces sterilization of PLLA scaffolds contaminated by E. coli.

2014

The common sterilization techniques are based on physical processes that involve, for example, the use of autoclaves or systems to radiation such as γ-rays that can cause a structural change of the polymer treated. Therefore, the use of supercritical carbon dioxide (scCO2) is an excellent alternative, as it does not induce any variation of biomaterials treated (Perrut M., 2012). It's a good candidate because is readily available at low cost, non-toxic and non-flammable, it has an easily accessible critical point (7.38 MPa and 304.2 K) and excellent transport properties and wettability (White A. et al., 2005). We report the development of a supercritical CO2 based process capable of steriliz…

Paintings as Solid Affective Scaffolds

2019

We humans continuously reshape the environment to alter, enhance, and sustain our affective lives. This two‐way modification has been discussed in recent philosophy of mind as affective scaffolding, wherein scaffolding quite literally means that our affective states are enabled and supported by environmental resources such as material objects, other people, and physical spaces. In this article, I argue that under certain conditions, paintings function as noteworthy affective scaffolds to their creators. I begin with a theoretical overview of affective niche construction and affective scaffolding. Then, based on the criteria of robustness, concreteness, and dependability, I specify a more re…

Characterization of a novel three-dimensional outgrowth model of human bronchial mucosa

2011

Recently it has been hypothesized that a reactivation of the epithelial-mesenchymal trophic unit (EMTU) of the respiratory mucosa, caused by extensive epithelial damage, may be responsible for the pathogenesis of asthma. However, most research on this subject so far has been limited by the unavailability of in vitro models correctly mimicking the three-dimensional architecture of the properly differentiated human bronchial mucosa. For this reason, we have developed a novel 3D outgrowth model, obtained from bronchial biopsies and composed of both human bronchial epithelial cells (goblet and columnar cells) and fibroblasts with their basement membrane. Here we present the preliminary data obt…

Pore size graded scaffold for selective cellular permeation

2014

A NOVEL APPROACH TO FULLY CHARACTERIZE FIBER NETWORK MORPHOLOGY OF PLANAR FIBROUS TISSUES AND SCAFFOLDS

2011

Comprendere come la struttura di uno scaffold influenzi la morfologia cellulare e predirne il comportamento meccanico sono divenuti obiettivi fondamentali nello sviluppo dei tessuti ingegnerizzati. In questo studio si presenta un algoritmo innovativo basato su analisi d’immagine. Vengono introdotti descrittori di micro architettura che identificano univocamente la topologia della rete di fibre: orientamento delle fibre, connettività, densità spaziale delle intersezioni e diametro. Le prestazioni dell’algoritmo sono state valutate utilizzando immagini di microscopio a scansione elettronica (SEM) di (1) scaffolds in poliestere uretano-urea prodotti per elettrospinning, (2) gel di collagene se…