Search results for " SIT"
showing 10 items of 2985 documents
Southern and fluorescent in situ hybridization detect three RAPD-generated PCR products useful as introgression markers in Petunia
1999
Fluorescent in situ hybridization (FISH) was used to reveal the intrachromosomal organization of 11 RAPD markers localized on the genetic map of Petunia hybrida. The cloned RAPD markers were analyzed by means of Southern hybridization to determine their level of sequence repetition and their specificity in different Petunia species with 2n=14 and 18 chromosomes. The same probes were then used in FISH experiments. Most of the RAPD clones studied showed high sequence repetition and no species specificity. Moreover, FISH analysis showed that these probes could belong to multilocus families as evidenced by the multiple FISH signals dispersed throughout the genome and present on every chromosome…
Spatial monitoring of gene activity in extraradical and intraradical developmental stages of arbuscular mycorrhizal fungi by direct fluorescent in si…
2008
International audience; Gene expression profiling based on tissue extracts gives only limited information about genes associated with complex developmental processes such as those implicated in fungal interactions with plant roots during arbuscular mycorrhiza development and function. To overcome this drawback, a direct fluorescent in situ RT-PCR methodology was developed for spatial mapping of gene expression in different presymbiotic and symbiotic structures of an arbuscular mycorrhizal fungus. Transcript detection was optimized by targeting the LSU rRNA gene of Glomus intraradices and monitoring expression of a stearoyl-CoA-desaturase gene that is consistently expressed at high levels in…
Reproductive site selection: evidence of an oviposition cue in a highly adaptive dipteran, Drosophila suzukii (Diptera: Drosophilidae)
2020
Abstract Drosophila suzukii (Matsumura) is a vinegar fly species that originates from Eastern Asia and has spread throughout Europe and the Americas since its initial detection in United States in 2008. Its relatively large, sclerotized, and serrated ovipositor enables the ability to penetrate ripening fruits, providing a protected environment for its egg and larval stages. Because the mechanism of oviposition site selection of D. suzukii is a matter of hypothesis, the aim of the present study was to elucidate behavioral and chemical aspects of short-range ovipositional site selection within the context of D. suzukii reproductive biology. The preference of D. suzukii to lay eggs on artifici…
Egalitarian mixed-species bird groups enhance winter survival of subordinate group members but only in high-quality forests
2020
AbstractOnly dominant individuals have unrestricted access to contested resources in group-living animals. In birds, subordinates with restricted access to resources may respond to intragroup contests by acquiring extra body reserves to avoid periods of food shortage. In turn, higher body mass reduces agility and increases predation and mortality risk to subordinates. Birds often live in hierarchically organized mixed-species groups, in which heterospecific individuals are considered to substitute for conspecifics as protection against predators at a significantly reduced competition cost. Crested tits (Lophophanes cristatus) and willow tits (Poecile montanus) form mixed-species groups duri…
Fungal Diversity in the Mediterranean Area
2020
The Special Issue entitled “Fungal Diversity in the Mediterranean Area” aimed at highlighting the role of various organisms in the Mediterranean habitat. The role of fungi at the root and phyllosphere level; the biodiversity in small island territories and the sea; rare forms of fungi never previously found; the commercial, food, and therapeutic value of some ascomycetes and basidiomycetes; the diversity related to fungi associated with galls on plants; and the important role of culture collection for the ex situ conservation of fungal biodiversity are the topics dealt with in this Special Issue.
Analysis of movement patterns and macrohabitat use in Hermann’s tortoises (Testudo hermanni hermanni, Gmelin 1789) reintroduced in a coastal area dom…
2016
The Hermann’s tortoise is a strictly European species found mostly in areas with Mediterranean and sub-Mediterranean climes and, especially the subspecies T. hermanni hermanni, has to deal with various threats which reduced its geographic distribution to only a few isolated populations. Thus, with the objective of recovering natural populations of the species, numerous reintroduction projects are being carried out throughout its historical and prehistorical geographic distribution. The aim of our study is to evaluate the success of the T. hermanni reintroduction project carried out in the Albufera de Valencia Natural Park since 2011, through the analysis of movements, home range sizes and h…
Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
2010
Abstract Background The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Component…
Evidence for specific, high-affinity binding sites for a proteinaceous elicitor in tobacco plasma membrane
1995
Abstract Binding of cryptogein, a proteinaceous elicitor, was studied on tobacco plasma membrane. The binding of the [125I]cryptogein was saturable, reversible and specific with an apparent Kd of 2 nM. A single class of cryptogein binding sites was found with a sharp optimum pH for binding at about pH 7.0. The high-affinity correlates with cryptogein concentrations required for biological activity in vivo.
Characterization of (3H) acifluorfen binding to purified pea etioplasts, and evidence that protoporphyrinogen oxidase specifically binds acifluorfen
1992
It is now generally accepted that protoporphyrinogen oxidase is the target-enzyme for diphenylether-type herbicides. Recent studies [Camadro, J-M., Matringe, M., Scalla, R. & Labbe, P. (1991) Biochem. J. 277, 17–21] have revealed that in maize, diphenyl ethers competitively inhibit protoporphyrinogen oxidase with respect to its substrate, protoporphyrinogen IX. In this study, we show that, in purified pea etioplast, [3H]acifluorfen specifically binds to a single class of high-affinity binding sites with an apparent dissociation constant of 6.2 ± 1.3 nM and a maximum density of 29 ± 5 nmol/g protein. [3H]Acifluorfen binding reaches equilibrium in about 1 min at 30°C. Half dissociation occurs…
Breeding success of the Great Tit Parus major in relation to attributes of natural nest cavities in a primeval forest
2015
An overlap in attributes of nest cavities used by Great Tit Parus major across Eurasia suggests similar nest site preferences within the geographical range, although the drivers of these preferences are unclear. To determine whether preferred cavities provide conditions enhancing successful reproduction, we investigated the breeding performance of Great Tits in relation to tree cavity characteristics using data collected during 2008–2011 in primeval conditions (Białowieża National Park, Poland). Here, tree cavities are diverse and superabundant but nesting birds are at risk from a variety of predators. According to expectations, nest losses were high (60 % of Great Tit nests failed), mostly…