Search results for " Separation"
showing 10 items of 724 documents
Efficient selection of silenced primary cells by flow cytometry
2007
Background: RNA interference has emerged as a new and potent tool to knockdown the expression of target genes and to investigate their functions. For short time experiments with mammalian cell lines, RNA interference is typically induced by transfecting small interfering RNAs (siRNAs). Primary cells constitute important experimental systems in many studies because of their similarity to their in vivo counterparts; however, transfection of these cells has been found to be difficult. As a consequence, RNA interference of primary cells may result in mixed phenotypes because of the simultaneous presence in the same preparation of transfected and nontransfected cells. This may be particularly in…
Emotional separation and detachment as two distinct dimensions of parent--adolescent relationships
2011
The study examined adolescents’ emotional separation and detachment from parents, analyzing their relations with connectedness and agency, with some aspects of self—other boundary regulation and with problem behavior. The participants were 331 Italian adolescents, aged from 16 to 19 years (mean age = 17.40, SD = 1.14). Separation and detachment were positively related; they were negatively related to connectedness; detachment was also negatively associated to agency. Emotional separation was negatively predicted by empathic concern, perspective-taking and separate self; emotional detachment was negatively predicted by empathic concern and self—other differentiation. Separation negatively p…
Modification of the Selectivity Properties of Tubular Ceramic Membranes after Alkaline Treatment
2017
International audience; This work focuses on the selectivity modification of ceramic membranes after a mild alkaline treatment. Filtration of pure saltwater solutions was carried out with commercial titania membranes before and after the treatment. After treatment, the rejection of NaF significantly decreased, while the rejection of NaCl and NaBr increased. Additionally, NaI and Na 2 SO 4 remained close to zero. Pore size and electrical charge being almost unchanged, only significant modifications in the dielectric effects can explain this modification of selectivity. Therefore, the surface chemistry and the interaction (nature and magnitude) with the solvent and with the species present in…
Determination of organophosphate flame retardants in soil and fish using ultrasound-assisted extraction, solid-phase clean-up, and liquid chromatogra…
2017
A solid–liquid extraction method in combination with high‐performance liquid chromatography and tandem mass spectrometry was developed and optimized for extraction and analysis of organophosphorus flame retardants in soil and fish. Methanol was chosen as the optimum extraction solvent, not only in terms of extraction efficiency, but also for its broader analyte coverage. The subsequent clean‐up by solid‐phase extraction is required to eliminate matrix coextractives and reduce matrix effects. Recoveries of the optimized method were 50–121% for soil and 47–123% for biota, both with high precision (RSDs <12% in soil and <23% in biota). The method limits of detection ranged from 0.06 to 0.20 ng…
Heat-transfer performance comparison between overlapped and woven spacers for membrane distillation
2016
Sustainable production of fresh water from seawater desalination is a problem of crucial importance nowadays. Recently, some desalination technologies are taking advantage from the coupling with renewable resources. Among emerging technologies, Membrane Distillation (MD) is considered as one of the most promising as it can be easily powered by solar thermal energy or waste-heat. As an emerging technology, efforts are required to optimize MD unit geometry and operating conditions in order to reduce fresh water production specific cost. Temperature polarization phenomenon is a well-known detrimental effect for the MD process. Spacers are traditionally used to enhance mixing and shrink tempera…
Simultaneous screening and quantification of aminoglycoside antibiotics in honey using mixed-mode liquid chromatography with quadrupole time-of-fligh…
2018
An analytical method based on liquid chromatography with quadrupole time-of-flight mass spectrometry has been developed for the simultaneous determination of six aminoglycoside antibiotics in honey. The sample pretreatment included extraction with aqueous trichloroacetic acid followed by solid-phase extraction on Strata-X polymeric reversed phase cartridges. Liquid chromatography separation was performed on an Obelisc R zwitterionic type mixed-mode column. An ionBooster™ heated electrospray source was used and showed enhanced ionization efficiency in comparison to a conventional electrospray source. The observed signal enhancement ranged from 3- (neomycin) to 16-fold (gentamicin C1). A data…
Signal detection for spread spectrum communication systems with gradient algorithm
2012
Retrieval process of original symbols of a spread spectrum based communication system is tried to be improved by Gradient Algorithm (GA) and Blind Source Separation (BSS) principles. Two simple schemes, based on two energy functions are presented. Time correlation properties of the channel are used as advantages in developing the filter coefficients for the receiver. Direct Sequence - Code Division Multiple Access (DS-CDMA) technique based system setup is used for algorithm testing purposes. This is identified as one of the most stable spread spectrum communication technique where most of the technologies developed for that are highly compatible with High Speed Packet Access (HSPA) transmis…
Independent component analysis based on symmetrised scatter matrices
2007
A new method for separating the mixtures of independent sources has been proposed recently in [Oja et al. (2006). Scatter matrices and independent component analysis. Austrian J. Statist., to appear]. This method is based on two scatter matrices with the so-called independence property. The corresponding method is now further examined. Simple simulation studies are used to compare the performance of so-called symmetrised scatter matrices in solving the independence component analysis problem. The results are also compared with the classical FastICA method. Finally, the theory is illustrated by some examples. peerReviewed
On Independent Component Analysis with Stochastic Volatility Models
2017
Consider a multivariate time series where each component series is assumed to be a linear mixture of latent mutually independent stationary time series. Classical independent component analysis (ICA) tools, such as fastICA, are often used to extract latent series, but they don't utilize any information on temporal dependence. Also financial time series often have periods of low and high volatility. In such settings second order source separation methods, such as SOBI, fail. We review here some classical methods used for time series with stochastic volatility, and suggest modifications of them by proposing a family of vSOBI estimators. These estimators use different nonlinearity functions to…
Blind Source Separation Based on Joint Diagonalization in R: The Packages JADE and BSSasymp
2017
Blind source separation (BSS) is a well-known signal processing tool which is used to solve practical data analysis problems in various fields of science. In BSS, we assume that the observed data consists of linear mixtures of latent variables. The mixing system and the distributions of the latent variables are unknown. The aim is to find an estimate of an unmixing matrix which then transforms the observed data back to latent sources. In this paper we present the R packages JADE and BSSasymp. The package JADE offers several BSS methods which are based on joint diagonalization. Package BSSasymp contains functions for computing the asymptotic covariance matrices as well as their data-based es…