Search results for " Shock"

showing 10 items of 691 documents

Proteomics Differentiate Between Thyroid-Associated Orbitopathy and Dry Eye Syndrome.

2015

PURPOSE: In patients with thyroid-associated orbitopathy (TAO), the dry eye syndrome occurs frequently, and symptoms and signs of both disorders overlap making early and accurate differential diagnosis difficult. A differentiation via specific markers is warranted. METHODS: Tear fluid samples of 120 subjects with TAO, TAO + dry eye, dry eye, and controls were collected. The samples were measured using matrix-assisted laser desorption ionization mass spectrometry. The identified proteins were tested with antibody microarrays. RESULTS: Proteomics identified deregulated proteins in TAO and dry eye. Compared with dry eye, proline-rich protein 1 (PROL1, P = 0.002); uridine diphosphate (UDP)-gluc…

AdultMaleProteomicsmedicine.medical_specialtyAntibody microarrayAdolescentDry Eye SyndromesBiologyGraves' ophthalmopathyDiagnosis DifferentialYoung AdultInternal medicineHeat shock proteinmedicineHumansAgedMiddle Agedmedicine.diseaseGraves OphthalmopathyEndocrinologySpectrometry Mass Matrix-Assisted Laser Desorption-IonizationTearsbiology.proteinTearsDry Eye SyndromesFemaleCystatinAntibodyBiomarkersAnnexin A1Investigative ophthalmologyvisual science
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Is it possible to predict which patients with mild pneumonias will develop hypoxemia?

2009

SummaryUsually, mortality due to mild community-acquired pneumonias (CAP) (Pneumonia severity index (PSI) classes I–III) is low (<3%), but the appearance of hypoxemia significantly increases mortality. Our aim was to determine the clinical parameters associated with risk factors of developing hypoxemia in subjects with mild CAP (PSI I–III) and the clinical outcomes of the hypoxemic group.We analyzed clinical characteristics and the outcomes of patients with mild CAP and hypoxemia (PaO2/FiO2<300), in a prospective, multicenter cohort study of 1195 patients.Mild pneumonias (PSI I–III) were found in 645 cases (53.9%), of which 217 (33.6%) presented hypoxemia according to a PaO2/FiO2<300. Patie…

AdultMalePulmonary and Respiratory Medicinemedicine.medical_specialtyAdolescentCommunity-acquired pneumoniamedicine.medical_treatmentPneumonia severity indexSeverity assessmentHypoxemiaHypoxemiaPulmonary Disease Chronic ObstructiveYoung AdultCommunity-acquired pneumoniaHumansMedicineHypoalbuminemiaHypoxiaAgedMechanical ventilationCOPDbusiness.industrySeptic shockPneumoniaMiddle Agedmedicine.diseaserespiratory tract diseasesSurgeryCommunity-Acquired InfectionsRadiographyPneumoniaRisk factorsAnesthesiaFemalemedicine.symptomEpidemiologic MethodsbusinessHypoalbuminemiacirculatory and respiratory physiologyRespiratory Medicine
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Synovial fluid-derivedYersinia-reactive T cells responding to human 65-kDa heat-shock protein and heat-stressed antigen-presenting cells

1991

Humoral and cellular immune reactions to heat-shock proteins have been implicated in the pathogenesis of arthritis. Heat-shock proteins occur in bacteria as well as all eukaryotes and have been highly conserved during evolution. Cross-reactivity between bacterial and human heat-shock proteins induced at the site of inflammation may underlie the pathogenesis of some forms of arthritis. In order to test this hypothesis, we raised and cloned a Yersinia-specific T cell line from the synovial fluid lymphocytes of a patient with Yersinia-induced reactive arthritis. From this line we obtained a CD4+ T cell clone that proliferated in response to Yersinia antigens and both to the mycobacterial and t…

AdultMaleSalmonella typhimuriumHot TemperatureT-LymphocytesT cellImmunologyDose-Response Relationship ImmunologicAntigen-Presenting CellsArthritisCross ReactionsBiologyArthritis ReactiveImmune systemTetanus ToxinAntigenHeat shock proteinCandida albicansSynovial FluidEscherichia colimedicineHumansImmunology and AllergySynovial fluidAntigen-presenting cellHeat-Shock ProteinsT lymphocytebeta-Galactosidasemedicine.diseaseYersiniaCell biologymedicine.anatomical_structureImmunologyEuropean Journal of Immunology
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A 66-kilodalton heat shock protein of Salmonella typhimurium is responsible for binding of the bacterium to intestinal mucus

1992

Salmonella typhimurium infections have increased during the last few years. However, the interplay of virulence factors in S. typhimurium pathogenesis is still poorly understood, particularly with regard to the mechanisms and components of the bacterium which are involved in its interaction with the intestinal mucus. We have observed that S. typhimurium is aggregated by incubation with colonic mucus (guinea pig model). To quantify this phenomenon, an aggregation assay was established. By using this assay, it was found that the aggregation profile of S. typhimurium strains freshly isolated from patients (age 9 and older) with salmonellosis correlated with the severity of the disease. An isol…

AdultMaleSalmonella typhimuriumSalmonellaAdolescentGuinea PigsImmunologyVirulencemedicine.disease_causeMicrobiologyBacterial AdhesionMicrobiologyFecesBacterial ProteinsIntestinal mucosaHeat shock proteinCentrifugation Density GradientmedicineAnimalsHumansIntestinal MucosaChildHeat-Shock ProteinsbiologyTemperatureAntibodies MonoclonalMiddle Agedbiology.organism_classificationEnterobacteriaceaeMucusInfectious DiseasesPolyclonal antibodiesbiology.proteinFemaleParasitologyBacteriaResearch ArticleInfection and Immunity
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Multiclonal Synovial T Cell Response toYersinia enterocoliticain Reactive Arthritis: TheYersinia61-kDa Heat-Shock Protein Is Not the Major Target Ant…

1993

The T cell response to bacterial antigens plays a major role in the pathogenesis of reactive arthritis (ReA) following enteric infections with Yersinia enterocolitica. To study the antigen specificity of the T cells at the site of inflammation, the response of cloned T cells from the synovial fluid of 2 patients with ReA to partially purified antigens of Yersinia enterocolitica was determined. The clones showed different patterns of response to various fractions, indicating a multiclonal response to Yersinia antigens, and these specificities differed in the 2 patients. Some T cells were specific for Y. enterocolitica; some cross-reacted with other enterobacteria. Proteins of 14 and 19 kDa c…

AdultMaleYersinia InfectionsT-LymphocytesT cellCross ReactionsBiologyYersiniaArthritis ReactiveMicrobiologyEpitopesAntigenHeat shock proteinProhibitinsSynovial FluidmedicineHumansImmunology and AllergyYersinia enterocoliticaChromatography High Pressure LiquidHeat-Shock ProteinsYersinia enterocoliticaAntigens BacterialImmunity CellularYersiniosismedicine.diseasebiology.organism_classificationInfectious Diseasesmedicine.anatomical_structureChromatography GelbacteriaElectrophoresis Polyacrylamide GelBacterial antigenSynovial membraneJournal of Infectious Diseases
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Utility of presepsin (sCD14-ST) as a diagnostic and prognostic marker of sepsis in the emergency department

2015

Presepsin (PSEP) is released during infectious diseases and can be detected in the blood. PSEP has shown promising results as sepsis marker. We examined the diagnostic and prognostic validity of PSEP in patients suspicious of sepsis on admission in the emergency department (ED). Methods One hundred twenty three patients with signs of SIRS and/or sepsis and 123 healthy individuals were enrolled. PSEP was determined on admission, after 8, 24 and 72 h. Results Mean PSEP concentrations of the control group and the patient group were 130 and 1945 pg/ml. PSEP differed between SIRS, sepsis, severe sepsis and septic shock and showed strong association with 30-day mortality ranging from 10.3% in the…

AdultMalemedicine.medical_specialtyAdolescentClinical BiochemistryLipopolysaccharide ReceptorsBiochemistrySepsisDiagnosis DifferentialYoung AdultInternal medicineSepsisDiagnosismedicineHumansYoung adultIntensive care medicineAPACHE IIbusiness.industrySeptic shockEmergency departmentBiochemistry (medical)PresepsinGeneral MedicineEmergency departmentOutcome predictionMiddle Agedmedicine.diseasePrognosisShock SepticPeptide FragmentsQuartileClose relationshipFemalebusinessOutcome predictionEmergency Service HospitalBiomarkers
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Electromagnetically Generated Extracorporeal Shock Wave Lithotripsy and Adjuvant Combined Oral Litholysis for Therapy of Symptomatic Gallbladder Ston…

1991

A prospective study was conducted to evaluate effectivity, problems and adverse effects of extracorporeal shock wave lithotripsy (ESWL) using a newly developed electromagnetic biliary lithotriptor (Lithostar Plus, Siemens, Erlangen, FRG) for the treatment of selected patients presenting with symptomatic cholecystolithiasis. In addition to generally accepted criteria for the selection of patients, gallbladder contractility was established and pigment stones were excluded by computed tomography (CT). 80 out of 486 patients (63 females, 17 males, mean age 36, range 17-76 years) were selected for ESWL using a standardized diagnostic program. 62 out of 80 patients participating in the study had …

AdultMalemedicine.medical_specialtyAdolescentmedicine.medical_treatmentAdministration OralGallbladder StoneLithotripsyChenodeoxycholic AcidCholelithiasisLithotripsymedicineHumansProspective StudiesAgedUltrasonographybusiness.industryGallbladderUrsodeoxycholic AcidGastroenterologyMiddle AgedCombined Modality TherapyExtracorporeal shock wave lithotripsySurgerymedicine.anatomical_structureFemaleRadiologybusinessAdjuvantBiliary tract diseaseDigestion
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High-energy extracorporeal shock wave treatment of nonunions.

2001

Forty-three consecutive patients who did not have healing of tibial or femoral diaphyseal and metaphyseal fractures and osteotomies for at least 9 months after injury or surgery were examined prospectively for use of high-energy extracorporeal shock waves. Former treatment modalities (cast, external fixator, plate osteosynthesis, limitation of weightbearing) remained unchanged. In all cases a 99m Technetium dicarboxyphosphonate regional two-phase bone scintigraphy was performed before one treatment with 3000 impulses of an energy flux density of 0.6 mJ/mm 2 . Radiologic and clinical followups were done at 4-week intervals starting 8 weeks after shock wave treatment. The success criterion wa…

AdultMalemedicine.medical_specialtyAdolescentmedicine.medical_treatmentNonunionLong boneBone healingOsteotomyHigh-Energy Shock WavesmedicineHumansOrthopedics and Sports MedicineFemurProspective StudiesAgedOsteosynthesismedicine.diagnostic_testbusiness.industryGeneral MedicineMiddle Agedmedicine.diseaseSurgeryTibial FracturesPseudarthrosismedicine.anatomical_structureBone scintigraphyFractures UnunitedSurgeryFemalebusinessFemoral FracturesClinical orthopaedics and related research
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Low-energy extracorporeal shock wave therapy for painful heel: a prospective controlled single-blind study.

1996

The aim of this prospective single-blind pilot study was to explore the pain-alleviating effect of low-energy extracorporeal shock wave therapy (ESWT) in painful heel associated with inferior calcaneal spurs. Thirty patients who suffered from persistent symptoms for more than 12 months qualified for low-energy ESWT and were assigned at random to two groups, real or simulated ESWT. Before beginning the treatment, any other therapy was stopped for a period of 6 weeks. The shock waves were applied by an experimental device allowing exact localization through an integrated fluoroscopy unit. Patients were treated three times at weekly intervals. Each time 1000 impulses of 0.06 mJ/mm2 were given …

AdultMalemedicine.medical_specialtyHeelExtracorporeal shock wave therapyPainPilot ProjectsLow energyLithotripsySingle-Blind StudyMedicineFluoroscopyHumansPain ManagementOrthopedics and Sports MedicineProspective StudiesFasciitisPain Measurementmedicine.diagnostic_testbusiness.industryUltrasoundGeneral MedicineMiddle Agedmedicine.diseaseSurgerymedicine.anatomical_structureTreatment OutcomePatient SatisfactionOrthopedic surgeryChronic DiseaseSurgeryFemaleHeelbusinessArchives of orthopaedic and trauma surgery
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Prognosis Biomarkers of Severe Sepsis and Septic Shock by 1H NMR Urine Metabolomics in the Intensive Care Unit.

2015

Early diagnosis and patient stratification may improve sepsis outcome by a timely start of the proper specific treatment. We aimed to identify metabolomic biomarkers of sepsis in urine by (1)H-NMR spectroscopy to assess the severity and to predict outcomes. Urine samples were collected from 64 patients with severe sepsis or septic shock in the ICU for a (1)H NMR spectra acquisition. A supervised analysis was performed on the processed spectra, and a predictive model for prognosis (30-days mortality/survival) of sepsis was constructed using partial least-squares discriminant analysis (PLS-DA). In addition, we compared the prediction power of metabolomics data respect the Sequential Organ Fai…

AdultMalemedicine.medical_specialtyMultivariate analysisProton Magnetic Resonance SpectroscopyScienceUrineUrinalysisGastroenterologylaw.inventionSepsisYoung AdultMetabolomicslawSepsisInternal medicinemedicineHumansMetabolomicsLeast-Squares AnalysisYoung adultIntensive care medicineAgedAged 80 and overMultidisciplinarybusiness.industrySeptic shockOrgan dysfunctionQRDiscriminant AnalysisMiddle Agedmedicine.diseaseShock SepticIntensive care unitIntensive Care UnitsMedicineFemalemedicine.symptombusinessBiomarkersResearch ArticlePLoS ONE
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