Search results for " Simulation"

The C-terminal region of the Hot1 transcription factor binds GGGACAAA-related sequences in the promoter of its target genes

2015

Response to hyperosmotic stress in the yeast Saccharomyces cerevisiae involves the participation of the general stress response mediated by Msn2/4 transcription factors and the HOG pathway. One of the transcription factors activated through this pathway is Hot1, which contributes to the control of the expression of several genes involved in glycerol synthesis and flux, or in other functions related to adaptation to adverse conditions. This work provides new data about the interaction mechanism of this transcription factor with DNA. By means of one-hybrid and electrophoretic mobility assays, we demonstrate that the C-terminal region, which corresponds to amino acids 610-719, is the DNA-bindi…

A novel process-based model of microbial growth: self-inhibition in Saccharomyces cerevisiae aerobic fed-batch cultures

2015

Microbial population dynamics in bioreactors depend on both nutrients availability and changes in the growth environment. Research is still ongoing on the optimization of bioreactor yields focusing on the increase of the maximum achievable cell density. A new process-based model is proposed to describe the aerobic growth of Saccharomyces cerevisiae cultured on glucose as carbon and energy source. The model considers the main metabolic routes of glucose assimilation (fermentation to ethanol and respiration) and the occurrence of inhibition due to the accumulation of both ethanol and other self-produced toxic compounds in the medium. Model simulations reproduced data from classic and new expe…

Assessment of Salmonella and Listeria monocytogenes level in ready-to-cook poultry meat: Effect of various high pressure treatments and potassium lac…

2014

International audience; The objective of this study was to develop a probabilistic model in order to determine the contamination level of Salmonella and Listeria monocytogenes in ready-to-cook poultry meat, after a high pressure (HP) treatment. The model included four steps: i) Reception of raw meat materials, mincing and mixing meat, ii) Partitioning and packaging into 200-g modified atmosphere packs, iii) High pressure treatment of the meat, and iv) Storage in chilled conditions until the end of the shelf-life. The model excluded the cooking step and consumption at consumer's home as cooking practices and heating times are highly variable. The initial contamination level of Salmonella and…

Poly-sarcosine and poly(ethylene-glycol) interactions with proteins investigated using molecular dynamics simulations

2018

Nanoparticles coated with hydrophilic polymers often show a reduction in unspecific interactions with the biological environment, which improves their biocompatibility. The molecular determinants of this reduction are not very well understood yet, and their knowledge may help improving nanoparticle design. Here we address, using molecular dynamics simulations, the interactions of human serum albumin, the most abundant serum protein, with two promising hydrophilic polymers used for the coating of therapeutic nanoparticles, poly(ethylene-glycol) and poly-sarcosine. By simulating the protein immersed in a polymer-water mixture, we show that the two polymers have a very similar affinity for the…

Analisi dell’effetto di una scabrezza irregolare sulle strutture turbolente

2011

Analisi dell'effetto di scabrezze irregolari 2D e 3D sui campi di moto turbolenti.

2012

Dissipative scaling of step-pool features

2021

Abstract This paper focuses on the dissipative similarity of step-pool units at rill, flume and stream scale. This investigation is carried out using recent advances in open channel flow resistance, applications of close-range photogrammetry to rill erosion, available published data on step-pool features in flumes and streams and a new dataset of measurements in fixed bed step-pool rills. A theoretically-based equation for calculating the Darcy-Weisbach friction factor obtained by integration of a power velocity profile is presented. The scale factor Γ of this power velocity profile, which is included in the flow resistance equation, was previously calibrated (Eq. 10) for mobile bed rills w…

Is there any scaling in the cluster distribution?

1994

We apply fractal analysis methods to investigate the scaling properties in the Abell and ACO catalogs of rich galaxy clusters. We also discuss different technical aspects of the method when applied to data sets with small number of points as the cluster catalogs. Results are compared with simulations based on the Zel'dovich approximation. We limit our analysis to scales less than 100 $\hm$. The cluster distribution show a scale invariant multifractal behavior in a limited scale range. For the Abell catalog this range is 15--60$\hm$, while for the ACO sample it extends to smaller scales. Despite this difference in the extension of the scale--range where scale--invariant clustering takes plac…

Finding Possible Weakness in the Runoff Simulation Experiments to Assess Rill Erosion Changes without Non-Intermittent Surveying Capabilities

2020

The Terrestrial Photogrammetry Scanner (TEPHOS) offers the possibility to precisely monitor linear erosion features using the Structure from Motion (SfM) technique. This is a static, multi-camera array and dynamically moves the digital videoframe camera designed to obtain 3-D models of rills before and after the runoff experiments. The main goals were to (1) obtain better insight into the rills

Numerical study of the components positioning influence on the stability of a reverse shoulder prosthesis

2014

Aim of this paper is to setup a novel procedure able to analyze performances of a reverse shoulder prosthesis when different geometrical configurations are assumed. Nowadays, such a prosthesis is widely used but, because of its novelty, data in literature give poor information about performances and limits to its applicability. The activity has been divided into the following steps. At the beginning the shape of the prosthesis has been digitally acquired via a 3D scanner. Then, CAD models of all prosthetic components have been geometrically optimized in a way to obtain final entities suitable for numerical simulations. After that, CAD assemblies have been created between prosthetic componen…