Search results for " Size"

Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O

2001

The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10 5 –10 6 molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca 2+ -calmodulin and on intact microtubules, but was not sensitive to actin disruption or to inhibition of protein synthesis. Resealed cells were viable for days and retained the capacity to endocytose and to proliferate. The active domains of large clostridial toxins were introduced into three different cell lines. The domains were derived from Clostridium difficile B-toxin and Clo…

On overall sampling plan for small area estimation

2017

The time and budget restrictions in survey sampling can impose limits on the area sample sizes. This may reduce the possibility to obtain area-specific and population parameters estimates with adequate precision. Market research companies and institutes for producing official statistics face frequently this problem. Various models and methods for small area estimation (SAE) have been developed to solve this problem. The sample allocation must support the selected model and method to ensure efficient estimation and must be implemented in the design phase of the survey. The proposed allocation is developed by incorporating auxiliary information, a model, and an estimation method. The estimate…

Mācību sasniegumus ietekmējošie faktori Latvijas vispārizglītojošās skolās

2017

Promocijas darba mērķis ir izpētīt datus par izglītojamo mācību sasniegumiem starptautiski salīdzinošajos izglītības pētījumos, valsts pārbaudes darbos un izglītojamo gada vērtējumiem mācību priekšmetos, sniegt rekomendācijas par iespējamiem Latvijas vispārējās izglītības optimizācijas virzieniem. Promocijas darba rezultātā ir izstrādāti ierosinājumi, kuru īstenošana ļautu paaugstināt izglītojamo mācību sasniegumus vispārējās izglītības posmā. Promocijas darbu veido ievads, sešas savstarpēji saistītas nodaļas par četrām tēmām un nobeigums. Atslēgas vārdi: vispārējā izglītība, izglītības sistēmas optimizācija, mācību sasniegumi, kavējumi, skolas un klases lielums, obligātās izglītības uzsākš…

Hormone replacement therapy improves contractile function and myonuclear organization of single fibres from postmenopausal monozygotic female twin pa…

2013

Ageing is associated with a decline in muscle mass and strength leading to increased physical dependency in old age. Postmenopausal women experience a greater decline than men of similar age in parallel with the decrease in female sex steroid hormone production. We recruited six monozygous female twin pairs (55–59 years old) where only one twin pair was on hormone replacement therapy (HRT use = 7.8 ± 4.3 years) to investigate the association of HRT with the cytoplasmic volume supported by individual myonuclei (myonuclear domain (MND) size,) together with specific force at the single fibre level. HRT use was associated with a significantly smaller (∼27%; P < 0.05) mean MND size in muscle fib…

Implementation of IT applications in small and middle size enterprises : controlling the critical success factors

2002

Extraordinary Performance of Carbon‐Coated Anatase TiO 2 as Sodium‐Ion Anode

2015

The synthesis of in situ polymer‐functionalized anatase TiO2 particles using an anchoring block copolymer with hydroxamate as coordinating species is reported, which yields nanoparticles (≈11 nm) in multigram scale. Thermal annealing converts the polymer brushes into a uniform and homogeneous carbon coating as proven by high resolution transmission electron microscopy and Raman spectroscopy. The strong impact of particle size as well as carbon coating on the electrochemical performance of anatase TiO2 is demonstrated. Downsizing the particles leads to higher reversible uptake/release of sodium cations per formula unit TiO2 (e.g., 0.72 eq. Na+ (11 nm) vs only 0.56 eq. Na+ (40 nm)) while the …

Bi-2212 and Y123 highly curved single-crystal-like objects: whiskers, bows and ring-like structures

2012

High-temperature superconducting objects of Bi2Sr2CaCu2O8 and Y Ba2Cu3O7 highly curved in the ab-plane, such as curved/kinked whiskers, bows and ring-like structures, were obtained within a solid?liquid?solid (SLS) grass-like growth mechanism. As-grown objects are crystals with three-dimensional epitaxy similar to conventional single crystals: they can be viewed as crystal parts ?cut? from a conventional rectangular crystal. Between our curved objects and conventional crystals, whiskers or thin films there are some differences in the superconducting properties induced only by the shape factors and no new physics is observed. Some details of the growth mechanism are discussed, emphasizing cu…

Population divergence in maternal investment and embryo energy use and allocation suggests adaptive responses to cool climates

2023

1. The thermal sensitivity of early life stages can play a fundamental role in constraining species distributions. For egg-laying ectotherms, cool temperatures often extend development time and exacerbate developmental energy cost. Despite these costs, egg laying is still observed at high latitudes and altitudes. How embryos overcome the developmental constraints posed by cool climates is crucial knowledge for explaining the persistence of oviparous species in such environments and for understanding thermal adaptation more broadly. 2. Here, we studied maternal investment and embryo energy use and allocation in wall lizards spanning altitudinal regions, as potential mechanisms that enable su…

Spatial and temporal variation in larval density of coregonids and their consequences for population size estimation in Finnish lake

2011

Variazioni temporali della struttura di taglia in popolazioni sfruttate di Haliotis tuberculata (Mollusca Gastropoda) nel Golfo di Sferracavallo (PA)

2007

This study aims at evaluating the differences in size structure of population of H. tuberculata in the Gulf of Sferracavallo (northern Sicily) which experienced the co-occurrence of high exploitation and decrease of eutrophic conditions during the eighties and early nineties. Comparison of size structure showed significant differences between length frequency distributions of 1970-75s and 2002-2005, being the latter formed by smaller size green ormer.