Search results for " Tissue engineering"
showing 10 items of 103 documents
Micro - Architectural Data Extraction for Electrospun Poly (Ester Urethane) Urea Scaffolds for Biomechanical Modeling.
2009
Problem: Soft tissue engineered applications have raised the need for accurate descriptions of tissue microstructure and their contributions to global mechanical behavior [1]. Accurate material image analysis is crucial to model engineered tissue biomechanics. The present study proposes a novel method to automatically collect micro-architectural data from electron micrographs (SEM) of electrospun poly (ester urethane) urea (PEUU). Methods: Sets of contiguous SEM images for electrospun PEUU scaffolds made using three mandrel collection tangential velocities (1.5, 4.5, 9.0 m/s) were analyzed. A combination of thresholding and morphological procedures enabled overlaps of fibers to be detected.…
Poly-left-lactic acid tubular scaffolds via diffusion induced phase separation: Control of morphology
2013
n this work, tubular poly-left-lactic acid scaffolds for vascular tissue engineering applications were produced by an innovative two-step method. The scaffolds were obtained by performing a dip-coating around a nylon fiber, followed by a diffusion induced phase separation process. Morphological analysis revealed that the internal lumen of the as-obtained scaffold is equal to the diameter of the fiber utilized; the internal surface is homogeneous with micropores 1–2 μm large. Moreover, a porous open structure was detected across the thickness of the walls of the scaffold. An accurate analysis of the preparation process revealed that it is possible to tune up the morphology of the scaffold (w…
Tubular scaffold for vascular tissue engineering application
2010
A critical obstacle in tissue engineering is the inability to maintain large masses of living cells upon transfer from the in vitro culture conditions into the host in vivo. Capillaries, and the vascular system, are required to supply essential nutrients, including oxygen, remove waste products and provide a biochemical communication “highway”. Another goal in this research field is the possibility to tune the biodegradability of the scaffold. After implantation, the scaffold has to be gradually replaced by cells and extra cellular matrix and it is crucial that this replacement takes place with an appropriate dynamics. A premature degradation, in fact, could lead to a collapse of the struct…
PLLA scaffolds based on thermally induced phase separation: morphology, cell seeding and proliferation
2009
Decellularized Saphena: Biologic Scaffold for 3D Cellular Growth
2016
A tissue-engineered artificial bile duct grown to resemble the native bile duct in a porcine model
Biogenic Inorganic Polysilicates (Biosilica): Formation and Biomedical Applications
2013
The siliceous sponges, the demosponges and hexactinellid glass sponges, are unique in their ability to form biosilica structures with complex architectures through an enzyme-catalyzed mechanism. The biosilica skeleton of these sponges with its hierarchically structure and exceptional opto-mechanical properties has turned out to be an excellent model for the design of biomimetic nanomaterials with novel property combinations. In addition, biosilica shows morphogenetic activity that offers novel applications in the field of bone tissue engineering and repair. In recent years, much progress has been achieved towards the understanding of the principal enzymes, the silicateins that form the spon…
Tailoring Electrospinning Fabrication for Scaffolds for Heart Valve Tissue Engineering
2010
Previous work in controlling mechanical properties of electrospun scaffolds has largely been limited to altering the orientation of the fibrous network by either large rotational velocity or by altering the electric field during fabrication. Our lab has previously developed a technique to analyze the complete microstructural topology of electrospun scaffolds and extract key descriptors. In this project, we translated the target mandrel at varying speeds along its rotational axis in order to modify the microarchitecture without altering the fiber orientation angle. Using the algorithm mentioned above, we determined that increasing the translation speed resulted in a decrease in fiber interse…
Editorial - Connecting the Dots: The Promises of Wharton's Jelly Mesenchymal Stem Cells for Tissue Repair and Regeneration
2011
Mesenchymal stem cells (MSC) constitute a variety of cellular populations which were described first about 35 years ago in the bone marrow (BM) stroma [1]. These years have foreseen an exponential increase of reports pointing out features, stemness, markers, tissue sources and clinical applications of these cells. Cells with MSC features can be isolated from virtually every adult organ in the body, as well from a group of fetus-associated sources (cells derived from the latter tissues are collectively known as perinatal stem cells) [2]. In recent years, the umbilical cord arose as a promising source of mesenchymal stem cells, which can be isolated in relatively high numbers (compared to BM)…