Search results for " Type 3"

showing 9 items of 19 documents

Subtype selective binding properties of substituted linear melanocyte stimulating hormone analogues

2002

The melanocortin receptors are peptide binding G-protein coupled receptors that play a role in important physiological functions such as energy balance, inflammatory processes and several aspects of reproduction. In this study, we synthesised 11 new linear MSH analogues and tested their binding to the human MC receptors (MC1, MC3, MC4 and MC5) expressed in COS cells. Our results show that introduction of Asp in position 4 similarly affects the binding to the MC1, MC4 and MC5 receptors, but drastically lowers the binding to the MC3 receptor. Arg(5) substitution shows relatively high affinity for the MC4 receptor, while the results also give further support for specific importance of His(6) f…

Melanocyte-stimulating hormonePeptide bindingTransfectionBinding CompetitiveCell LineCellular and Molecular NeuroscienceEndocrinologyMelanocortin receptorHumans5-HT5A receptorMelanocyte-Stimulating HormonesReceptorCOS cellsEndocrine and Autonomic SystemsChemistryReceptors MelanocortinGeneral MedicineTransfectionAmino Acid SubstitutionReceptors CorticotropinNeurologyBiochemistryReceptor Melanocortin Type 4MelanocortinReceptor Melanocortin Type 1Receptor Melanocortin Type 3Neuropeptides
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Redox signaling in acute pancreatitis

2015

Acute pancreatitis is an inflammatory process of the pancreatic gland that eventually may lead to a severe systemic inflammatory response. A key event in pancreatic damage is the intracellular activation of NF-κB and zymogens, involving also calcium, cathepsins, pH disorders, autophagy, and cell death, particularly necrosis. This review focuses on the new role of redox signaling in acute pancreatitis. Oxidative stress and redox status are involved in the onset of acute pancreatitis and also in the development of the systemic inflammatory response, being glutathione depletion, xanthine oxidase activation, and thiol oxidation in proteins critical features of the disease in the pancreas. On th…

NecrosisGSH reduced glutathioneSTAT3 signal transducer and activator of transcription 3ERK extracellular signal-regulated kinasesClinical BiochemistryCCK cholecystokininTRAFs TNF receptor associated factorsReview ArticleIκB kinasePharmacologymedicine.disease_causeBiochemistrySHP small heterodimer partnerSTIM1 stromal interaction molecule 1chemistry.chemical_compoundHATs histone acetyltransferasesMedicineASK1GCL glutamate cysteine ligaseTNF-α tumor necrosis factor alphaIKK IκB kinaseNOS nitric oxide synthaseAcute inflammationHIF hypoxia inducible factorlcsh:QH301-705.5NF-κB nuclear factor kappa BDAMPs damage-associated molecular pattern moleculeslcsh:R5-920biologyGSSG oxidized glutathioneNF-kappa BNLRs nucleotide-binding oligomerization domain (NOD) like receptorsTRADD tumor necrosis factor receptor type 1-associated DEATH domain proteinTRPC3 transient receptor potential channel 3VEGF vascular endothelial growth factorGlutathioneTNFR tumor necrosis factor receptorHMGB1 high-mobility group Box 1 proteinIP3R inositol 145-trisphosphate receptor type 3VCAM-1 Vascular Cell adhesion protein 1Acute DiseaseJNK c-Jun N-terminal kinaseAcute pancreatitisTLRs toll-like receptorsmedicine.symptomlcsh:Medicine (General)Oxidation-ReductionAP-1 activator protein-1Signal TransductionmRNA messenger ribonucleic acidHMGB1ASC apoptosis-associated speck-like protein containing a carboxy-terminal CARDRNS reactive nitrogen speciesPTPs protein tyrosine phosphatasesROS reactive oxygen speciesNADH nicotinamide adenine dinucleotidepHe extracellular pHFAEE fatty acid ethyl estersAP acute pancreatitisHumansXanthine oxidaseCBP CREB-binding proteinRyR endoplasmic reticulum membrane ryanodine receptorsMDA malondialdehydeNO nitric oxideXO xanthine oxidaseASK1 apoptosis signal-regulating kinase-1business.industryOrganic ChemistryAutophagyNADPH nicotinamide adenine dinucleotide phosphateHDACs histone deacetylasesmedicine.diseaseCARS compensatory anti-inflammatory response syndromeXDH xanthine dehydrogenaseIL interleukinIκB inhibitor of kappa BAcute pancreatitisETC Electron transport chainPancreatitisMKPs MAPK phosphatasesSAP severe acute pancreatitischemistrylcsh:Biology (General)DTT dithiothreitolOxidative stressNAC N-acetyl cysteineImmunologybiology.proteinCalciumLysosomesReactive Oxygen SpeciesbusinessMAPK mitogen-activated protein kinaseOxidative stressERCP endoscopic retrograde cholangiopancreatographyRedox Biology
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UNDERSTANDING THE RELATIONSHIP BETWEEN NORMAL FUNCTION AND ABERRANT AGGREGATION: THE CASE OF ATAXIN-3

2020

Polyglutamine diseaseSpinocerebellar Ataxia type 3Ubiquitin.Protein aggregationAtaxin-3Polyubiquitin chain
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Early OCTA Changes of Type 3 Macular Neovascularization Following Brolucizumab Intravitreal Injections

2022

Background and Objectives: Brolucizumab is a novel anti-vascular endothelial growth factor (VEGF), whose efficacy has been shown in the Hawk and Harrier phase 3 clinical studies. The goal of the present case series is to report initial results of brolucizumab intravitreal injections (IVI) on type 3 neovascularization in neovascular age-related macular degeneration (nAMD), evaluated by optical coherence tomography angiography (OCTA). Materials and Methods: This is a bicentric retrospective case series. Patients with newly diagnosed type 3 MNV treated with brolucizumab IVI and at least 6 months follow-up were enrolled. OCTA en face images and B-scans were analyzed for lesions at baseline, 1 m…

Vascular Endothelial Growth Factor Atype 3 neovascularization.brolucizumabNeovascularization PathologicVisual Acuityneovascular age-related macular degeneration; type 3 neovascularization; retinal angiomatous proliferation; brolucizumab; optical coherence tomography angiography; intravitreal injectionintravitreal injectionretinal angiomatous proliferationEndothelial Growth Factorsneovascular age-related macular degenerationGeneral MedicineAntibodies Monoclonal Humanizedoptical coherence tomography angiographyIntravitreal InjectionsHumansFluorescein AngiographyTomography Optical CoherenceRetrospective StudiesMedicina
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Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets

2020

Autosomal recessive mutations in genes required for cytotoxicity are causative of a life-threatening, early-onset hyperinflammatory syndrome termed familial hemophagocytic lymphohistiocytosis (FHL). Mutations in UNC13D cause FHL type 3. UNC13D encodes Munc13-4, a member of the Unc13 protein family which control SNARE complex formation and vesicle fusion. We have previously identified FHL3-associated mutations in the first intron of UNC13D which control transcription from an alternative transcriptional start site. Using isoform specific antibodies, we demonstrate that this alternative Munc13-4 isoform with a unique N-terminus is preferentially expressed in human lymphocytes and platelets, as…

lcsh:Immunologic diseases. Allergyfamilial hemophagocytic lymphohistiocytosis type 3lymphocyte cytotoxicityUNC13Dprimary immunodeficiencyintronic mutationlcsh:RC581-607alternative intronic promoter/isoformFrontiers in Immunology
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Cilostazol and atherogenic dyslipidemia: a clinically relevant effect

2011

Cilostazol is a reversible, selective inhibitor of PDE3A able to significantly improve walking distance in patients with intermittent claudication. However, beyond its antiplatelet and vasodilator properties, cilostazol seems to have significant effects on atherogenic dyslipidemia.The effects of cilostazol on plasma lipids, lipoproteins, apolipoproteins and postprandial lipemia are reviewed. A literature search (using Medline and Scopus) was performed up to 24 October 2010. The authors also manually reviewed the references of selected articles for any pertinent material.Cilostazol is able to significantly lower plasma triglyceride levels, with a concomitant increase in high-density lipoprot…

medicine.medical_specialtyApolipoprotein BTetrazolescilostazol atherogenic dyslipidemiaPhosphodiesterase 3 InhibitorsPeripheral Arterial Diseasechemistry.chemical_compoundDiabetes mellitusInternal medicineHumansMedicinePharmacology (medical)DyslipidemiasPharmacologymedicine.diagnostic_testbiologybusiness.industryCholesterolGeneral MedicineAtherosclerosismedicine.diseaseLipidsCyclic Nucleotide Phosphodiesterases Type 3Intermittent claudicationCilostazolCilostazolPostprandialEndocrinologyDiabetes Mellitus Type 2chemistrybiology.proteinlipids (amino acids peptides and proteins)medicine.symptombusinessLipid profileLipoproteinmedicine.drug
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Cloning of two melanocortin (MC) receptors in spiny dogfish

2004

We report the cloning and characterization of two melanocortin receptors (MCRs) from the spiny dogfish (Squalus acanthias) (Sac). Phylogenetic analysis shows that these shark receptors are orthologues of the MC3R and MC5R subtypes, sharing 65% and 70% overall amino acid identity with the human counterparts, respectively. The SacMC3R was expressed and pharmacologically characterized in HEK293 cells. The radioligand binding results show that this receptor has high affinity for adrenocorticotropic hormone (ACTH)-derived peptides while it has comparable affinity for alpha- and beta-melanocyte stimulating hormone (MSH), and slightly lower affinity for gamma-MSH when compared with the human ortho…

medicine.medical_specialtyGreen Fluorescent ProteinsMolecular Sequence DataCHO CellsAdrenocorticotropic hormoneBiologyPolymerase Chain ReactionBiochemistryCell LineRadioligand Assaygamma-MSHAdrenocorticotropic HormoneCricetinaeInternal medicineCyclic AMPEscherichia colimedicineAnimalsHumansPotencyBacteriophagesTissue DistributionAmino Acid SequenceMelanocyte-Stimulating HormonesCloning MolecularReceptorPhylogenyGene Librarychemistry.chemical_classificationSpiny dogfishDose-Response Relationship DrugSequence Homology Amino AcidReverse Transcriptase Polymerase Chain ReactionChinese hamster ovary cellHEK 293 cellsSequence Analysis DNAbiology.organism_classificationMolecular biologyIntronsAmino acidBlotting SouthernKineticsEndocrinologychemistryDogfishReceptor Melanocortin Type 4MelanocortinPeptidesReceptor Melanocortin Type 3European Journal of Biochemistry
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The phototransduction cascade in the isolated chick pineal gland revisited.

2003

It is well established that the isolated chick pineal gland is directly light sensitive and that melatonin synthesis of the gland can be inhibited by exposing the gland to light during scotophase. Since not all the steps of the phototransduction cascade have been clarified to the same extent as in the retina, we have treated isolated chick pineal glands with 90 min of light during scotophase and with drugs that affect key-components of vertebrate phototransduction, i.e., cyclic guanosine monophosphate (cGMP) phosphodiesterase 6 (PDE6), cGMP levels and cGMP-gated calcium channels. The endpoint measured was the activity of the rate-limiting enzyme of melatonin synthesis, arylalkylamine N-acet…

medicine.medical_specialtyLight Signal TransductionArylamine N-AcetyltransferasePhosphodiesterase 3BiologyNitric OxidePineal GlandRetinachemistry.chemical_compoundPineal glandOrgan Culture TechniquesInternal medicinemedicineCyclic AMPAnimalsCyclic adenosine monophosphateNitric Oxide DonorsEnzyme InhibitorsMolecular BiologyCyclic guanosine monophosphateCyclic GMPMelatoninCyclic Nucleotide Phosphodiesterases Type 6Phosphoric Diester HydrolasesGeneral NeurosciencePhosphodiesteraseNatriuretic Peptide C-TypeCyclic Nucleotide Phosphodiesterases Type 3Circadian RhythmCalcium Channel Agonistsmedicine.anatomical_structureEndocrinologychemistry3'5'-Cyclic-AMP PhosphodiesterasesNeurology (clinical)PDE10ACalcium ChannelsZaprinastChickensPhotic StimulationDevelopmental BiologyEndocrine glandBrain research
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Pharmacological comparison of rat and human melanocortin 3 and 4 receptors in vitro.

2002

Abstract The melanocortin 3 and 4 receptors are G-protein-coupled receptors found in the hypothalamus with important role in regulation of the energy balance. In this study, we performed pharmacological comparison of the rat and human melancortin (MC) 3 and MC4 receptors. We transiently expressed the genes for these receptors individually in a mammalian cell line and determined the binding affinities to several MSH peptides. The results showed no major difference between the rat and human MC3 receptors while the rat MC4 receptor had higher affinity to several peptides compared with the human MC4 receptor. NDP-, α-, β-, γ-MSH, ACTH(1–24), HS014 and MTII had from 5- to 34-fold higher affinity…

medicine.medical_specialtyPhysiologyClinical BiochemistryHypothalamusClass C GPCRBiologyLigandsBiochemistryBinding CompetitiveCellular and Molecular NeuroscienceChemokine receptorEndocrinologyMelanocortin receptorInternal medicinemedicineCyclic AMPAnimalsHumansACTH receptorReceptor5-HT receptor5-HT2 receptorCell biologyRatsEndocrinologyReceptors Corticotropinalpha-MSHCOS CellsReceptor Melanocortin Type 45-HT1 receptorProtein BindingReceptor Melanocortin Type 3Regulatory peptides
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