Search results for " UNIVERSITY"

showing 10 items of 681 documents

Development of an RNA-based kit for easy generation of TCR-engineered lymphocytes to control T-cell assay performance.

2018

Cell-based assays to monitor antigen-specific T-cell responses are characterized by their high complexity and should be conducted under controlled conditions to lower multiple possible sources of assay variation. However, the lack of standard reagents makes it difficult to directly compare results generated in one lab over time and across institutions. Therefore TCR-engineered reference samples (TERS) that contain a defined number of antigen-specific T cells and continuously deliver stable results are urgently needed. We successfully established a simple and robust TERS technology that constitutes a useful tool to overcome this issue for commonly used T-cell immuno-assays. To enable users t…

0301 basic medicineRNA StabilityComputer scienceT cellPerformanceCancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2]RNA StabilityT-LymphocytesImmunologyCell Culture TechniquesComputational biology03 medical and health sciences0302 clinical medicineAll institutes and research themes of the Radboud University Medical CenterHigh complexityValidationHLA-A2 AntigenmedicineImmunology and AllergyHumansImrnunoguidingRNA MessengerCell EngineeringT-cell assaysReceptors Chimeric AntigenImmunomagnetic SeparationElectroporationT-cell receptorRNAReference StandardsStandardizationImmunomonitoring030104 developmental biologymedicine.anatomical_structureElectroporationBlood Buffy CoatFeasibility StudiesBiological Assay030215 immunologyJournal of immunological methods
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Usherin defects lead to early-onset retinal dysfunction in zebrafish

2018

Mutations in USH2A are the most frequent cause of Usher syndrome and autosomal recessive nonsyndromic retinitis pigmentosa. To unravel the pathogenic mechanisms underlying USH2A-associated retinal degeneration and to evaluate future therapeutic strategies that could potentially halt the progression of this devastating disorder, an animal model is needed. The available Ush2a knock-out mouse model does not mimic the human phenotype, because it presents with only a mild and late-onset retinal degeneration. Using CRISPR/Cas9-technology, we introduced protein-truncating germline lesions into the zebrafish ush2a gene (ush2a(rmc1): c.2337_2342delinsAC; p.Cys780GlnfsTer32 and ush2a(b1245): c.15520_…

0301 basic medicineRetinal degenerationGenotyping TechniquesUsher syndrome2804 Cellular and Molecular NeuroscienceApoptosis030105 genetics & heredityBiologyArticleRetinaGermlineSensory disorders Donders Center for Medical Neuroscience [Radboudumc 12]Gene Knockout Techniques03 medical and health sciencesCellular and Molecular NeuroscienceUSH2 complex2809 Sensory SystemsAll institutes and research themes of the Radboud University Medical CenterRetinitis pigmentosaElectroretinographymedicineotorhinolaryngologic diseasesJournal ArticleAnimalsMicroscopy ImmunoelectronZebrafishZebrafishExtracellular Matrix ProteinsRetinal DegenerationMembrane ProteinsZebrafish ProteinsRetinal Photoreceptor Cell Outer Segmentmedicine.diseasebiology.organism_classification2731 OphthalmologySensory Systems10124 Institute of Molecular Life SciencesCell biologyDisease Models AnimalOphthalmology030104 developmental biologyGene Expression RegulationEctodomainMutation570 Life sciences; biologyXenotropic and Polytropic Retrovirus ReceptorUsher SyndromesErg
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Detection of temporal clusters of health care-associated infections or colonizations with Pseudomonas aeruginosa.

2016

International audience; We investigated temporal clusters of Pseudomonas aeruginosa cases between 2005 and 2014 in 1 French university hospital, overall and by ward, using the Kulldorff method. Clusters of positive water samples were also investigated at the whole hospital level. Our results suggest that water outlets are not closely involved in the occurrence of clusters of P aeruginosa cases.

0301 basic medicinemedicine.medical_specialtyEpidemiology030106 microbiology030501 epidemiologymedicine.disease_causeHealth care associatedMicrobiologyHospitals University03 medical and health sciences[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyInternal medicinemedicineCluster AnalysisHumansPseudomonas InfectionsCross InfectionPseudomonas aeruginosabusiness.industryHealth PolicyPublic Health Environmental and Occupational HealthWaterHospital levelUniversity hospital3. Good healthInfectious Diseases[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyCarrier StatePseudomonas aeruginosaFranceWater Microbiology0305 other medical sciencebusiness
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Short-course radiotherapy followed by chemotherapy before total mesorectal excision (TME) versus preoperative chemoradiotherapy, TME, and optional ad…

2021

BACKGROUND: Systemic relapses remain a major problem in locally advanced rectal cancer. Using short-course radiotherapy followed by chemotherapy and delayed surgery, the Rectal cancer And Preoperative Induction therapy followed by Dedicated Operation (RAPIDO) trial aimed to reduce distant metastases without compromising locoregional control. METHODS: In this multicentre, open-label, randomised, controlled, phase 3 trial, participants were recruited from 54 centres in the Netherlands, Sweden, Spain, Slovenia, Denmark, Norway, and the USA. Patients were eligible if they were aged 18 years or older, with an Eastern Cooperative Oncology Group (ECOG) performance status of 0-1, had a biopsy-prove…

0301 basic medicinemedicine.medical_specialtyPopulationlaw.inventionTumours of the digestive tract Radboud Institute for Health Sciences [Radboudumc 14]03 medical and health sciencesFolinic acidAll institutes and research themes of the Radboud University Medical Center0302 clinical medicineRandomized controlled triallawTumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14]medicineClinical endpointeducationeducation.field_of_studyPerformance statusbusiness.industryTotal mesorectal excisionOxaliplatinSurgery030104 developmental biologyOncology030220 oncology & carcinogenesisbusinessChemoradiotherapymedicine.drugThe Lancet Oncology
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Randomized controlled trial comparing embryo culture in two incubator systems: G185 K-System versus EmbryoScope

2017

Objective To study whether the closed culture system, as compared with a benchtop incubator with similar culture conditions, has a positive impact on intracytoplasmic sperm injection (ICSI) outcomes. Design Randomized controlled trial. Setting University hospital. Patient(s) A total of 386 patients undergoing ICSI cycles with at least six mature oocytes were randomized. Intervention(s) Of these patients, 195 were assigned to the group with culture in a time-lapse imaging (TLI) system (EmbryoScope) and 191 to the group with culture in the G185 K-System (G185). Main Outcome Measure(s) Rate of implantation (primary endpoint) and embryo morphology grade. Result(s) No significant differences wer…

0301 basic medicinemedicine.medical_specialtyTime Factorsmedicine.medical_treatmentBiologyTime-Lapse ImagingIntracytoplasmic sperm injectionlaw.inventionEmbryo Culture TechniquesHospitals UniversityIncubators03 medical and health sciences0302 clinical medicineRandomized controlled trialEmbryo cryopreservationPregnancyRisk FactorslawOdds RatiomedicineClinical endpointHumansEmbryo ImplantationProspective StudiesSperm Injections IntracytoplasmicGynecologyMicroscopy Video030219 obstetrics & reproductive medicineObstetrics and GynecologyEmbryo cultureEmbryoEquipment DesignEmbryo TransferConfidence intervalEmbryo transferLogistic ModelsTreatment Outcome030104 developmental biologyReproductive MedicineInfertilityMultivariate AnalysisFemaleFranceMaternal AgeFertility and Sterility
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Prevalence of Age-Related Macular Degeneration in Europe

2017

Manuscript no. 2016-1147 Supplemental material is available at www.aaojournal.org/; International audience; [u]Purpose:[/u] Age-related macular degeneration (AMD) is a frequent, complex disorder in elderly of European ancestry. Risk profiles and treatment options have changed considerably over the years, which may have affected disease prevalence and outcome. We determined the prevalence of early and late AMD in Europe from 1990 to 2013 using the European Eye Epidemiology (E3) consortium, and made projections for the future. [u]Design:[/u] Meta-analysis of prevalence data. [u]Participants:[/u] A total of 42 080 individuals 40 years of age and older participating in 14 population-based cohor…

0301 basic medicinemedicine.medical_specialtyVisual acuityOPTICAL COHERENCE TOMOGRAPHYMACULOPATHYgenetic structuresPopulationPrevalenceHEART-DISEASEchoroidal neovascularizationSensory disorders Donders Center for Medical Neuroscience [Radboudumc 12]03 medical and health sciencesRotterdam Study0302 clinical medicineAll institutes and research themes of the Radboud University Medical CenterBEAVER DAM EYEEpidemiologygeographic atrophymedicineVISUAL IMPAIRMENT[SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory OrganseducationPOPULATIONeducation.field_of_studyBIRTH COHORTbusiness.industryMacular degenerationmedicine.diseaseTRENDSConfidence intervaleye diseases3. Good healthEuropean Prospective Investigation into Cancer and NutritionOphthalmology030104 developmental biologyAge-related Macular DegenerationENDOTHELIAL GROWTH-FACTORBLINDNESS030221 ophthalmology & optometryOptometryAge-related Macular Degeneration choroidal neovascularization geographic atrophysense organsmedicine.symptombusiness[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyDemography
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The VIRSTA score, a prediction score to estimate risk of infective endocarditis and determine priority for echocardiography in patients with Staphylo…

2016

International audience; Objectives - To develop and validate a prediction score, to quantify, within 48 h of Staphylococcus aureus bacteremia (SAB) diagnosis, the risk of IE, and therefore determine priority for urgent echocardiography. Methods - Consecutive adult patients with SAB in 8 French university hospitals between 2009 and 2011 were prospectively enrolled and followed-up 3 months. A predictive model was developed and internally validated using bootstrap procedures. Results - Among the 2008 patients enrolled, 221 (11.0%) had definite IE of whom 39 (17.6%) underwent valve surgery, 25% of them within 6 days of SAB diagnosis. Ten predictors independently associated with IE were used to …

0301 basic medicinemedicine.medical_treatmentBacteremiaHospitals University0302 clinical medicine[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyInterquartile rangeVertebral osteomyelitis030212 general & internal medicineLongitudinal StudiesProspective StudiesChildbiologyEndocarditisHeartMiddle AgedStaphylococcal InfectionsImplantable cardioverter-defibrillator3. Good healthInfectious Diseases[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyEchocardiographyInfective endocarditisChild PreschoolPrognostic scoreFranceMeningitisVIRSTA scoreMicrobiology (medical)Adultmedicine.medical_specialtyStaphylococcus aureusAdolescent030106 microbiologyRisk AssessmentDecision Support Techniques03 medical and health sciencesYoung AdultInternal medicinemedicineHumans[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyAgedbusiness.industryC-reactive proteinmedicine.diseaseConfidence intervalSurgeryBacteremiabiology.proteinInfective endocarditisStaphylococcus aureus bacteremiabusiness
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Actitudes docentes hacia los ejercicios de la Prueba de Acceso a la Universidad informatizada

2017

Resumen El presente artículo forma parte de la investigación desarrollada en el proyecto Paulex Universitas con la intención de obtener información sobre los ítems más aceptados por los profesores de 2º de Bachillerato de cara al inicio de la fase de pruebas para examinar la viabilidad de la puesta en funcionamiento de la Prueba de Acceso a la Universidad (PAU) asistida por ordenador en España y, más específicamente, los siguientes aspectos: la posibilidad de automatización de la prueba, las tipologías de ejercicios más populares entre los profesores y la relación entre las mismas. Un total de 214 profesores respondieron a un cuestionario diseñado según el método Delphi, destinado a averigu…

060201 languages & linguisticsInglés como lengua extranjera05 social sciences050301 educationActitudes de los profesores06 humanities and the artsComputer-Assisted Testinglcsh:Education (General)Education0602 languages and literatureSpanish University Entrance Examination (PAU)Tipología de ejerciciosEvaluación asistida por ordenadorTeachers’ attitudesExercise typologyPrueba de Acceso a la Universidad (PAU)lcsh:L7-9910503 educationEnglish as a Foreign LanguageEducação e Pesquisa
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Sociología, Derecho y desigualdades sociales. Una propuesta de docencia universitaria crítica

2019

Fil: Di Nella, Dino. Universidad Nacional de Río Negro. Río Negro, Argentina. Fil: Almeda, Elisabet. Universidad de Barcelona. Catalunya, España. Fil: Camps Calvet, Clara. Universidad de Barcelona. Catalunya, España. Fil: Giordana, Patricia. Universidad Nacional de Río Negro. Río Negro, Argentina. Fil: Loggiacco, Camila. Universidad Nacional de Río Negro. Río Negro, Argentina. Fil: D'Alfonso, Luis. Universidad Nacional de Río Negro. Río Negro, Argentina. Critic education in human rights and social inequalities must be able to capture holistic and mutidmiensional sphere that Human Rights requires. This study introduces the basis in which critic university teaching proposal is carectarized by…

1137-7038 8537 Arxius de sociologia 525290 2019 40 7038141 SociologíaUniversitiesAlmeda SamaranchCiencias SocialesUniversitatsDrets socials i econòmicsteam workUniversidadesD'Alfonsohuman rightsspecially relevant ones in this field: professional interdisciplinaryenseñanza de la sociología y el derechoGestió de la innovacióSociologyDerecho y desigualdades sociales. Una propuesta de docencia universitaria crítica Di NellaCamps CalvetCamillaUNESCO::SOCIOLOGÍATeaching innovationprofessional interdisciplinary [specially relevant ones in this field]DinoGestión de la innovaciónproblem solving and the utilization of ICTs. Innovación docentedesigualdades socialesteaching of sociology and lawLoggiacco:SOCIOLOGÍA [UNESCO]Derechos sociales y económicosGiordanaDerecho (general)ClaraSociologiasocial inequalities. 65 70ElisabetLuis Critic education in human rights and social inequalities must be able to capture holistic and mutidmiensional sphere that Human Rights requires. This study introduces the basis in which critic university teaching proposal is carectarized by different active learning mechanismes with the students for the achivement of four transversal competencesSocial and economic rightsPensamiento críticoderechos humanosSociologíaPensament críticCritical thinkingInnovation managementPatricia
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Latvijas nacionalās valsts idejas tapšana un viņas izpausmes veidi: (1892.g.-1919.gada 18. novembrim), 2. daļa

1935

1905. gada revolūcijaLatvijas neatkarībaHistory of the University of LatviaFilozofiKanta filozofija - P. Zālītes intrepretācijaLettische Philosophen:HUMANITIES and RELIGION::History and philosophy subjects::Philosophy subjects [Research Subject Categories]Zālīte Pēteris - memuāriMājas ViesisLatvijas Universitātes vēstureDienas LapaLatvijas Universitātes profesoriLatvijas vēsture
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