Search results for " Western"
showing 10 items of 687 documents
Expression of 60-kD Heat Shock Protein Increases during Carcinogenesis in the Uterine Exocervix
2002
<i>Objectives:</i> The aim of the present study was to determine the presence and expression of the 60-kD heat shock protein (HSP60) in the dysplasia-carcinoma sequence in the uterine exocervix and to evaluate its diagnostic and prognostic significance. <i>Methods and Results:</i> We performed Western blot and immunohistochemical analyses on biopsies from 40 cases, consisting of 10 normal exocervical biopsies, 10 low-grade squamous intraepithelial lesions (L-SIL), 10 high-grade squamous intraepithelial lesions (H-SIL) and 10 cancerous exocervices (G2 grade). The immunohistochemical results were quantified by computer-assisted image analysis. Western blot analysis sho…
Myopathy with hexagonally cross-linked crystalloid inclusions: delineation of a clinico-pathological entity.
2010
A novel myopathy characterized by hexagonally cross-linked tubular arrays has been reported in five patients. We studied the clinical and histopathological features of five additional unrelated patients with this myopathy. Patients experienced exercise intolerance with exercise-induced myalgia and weakness, without rhabdomyolysis. One patient additionally presented mild permanent pelvic girdle muscle weakness. Age at onset varied between 13 and 56 years. The inclusions were eosinophilic on H and E, bright red with modified Gomori’s trichrome stains, present in type 2 fibers, and revealed immunoreactivity selectively for a caveolin-3-antibody. Ultrastructurally, the inclusions showed a highl…
Eight new mutations and the expanding phenotype variability in muscular dystrophy caused by ANO5.
2012
Objective: Description of 8 new ANO5 mutations and significant expansion of the clinical phenotype spectrum associated with previously known and unknown mutations to improve diagnostic accuracy. Methods: DNA samples of 101 patients in 95 kindreds at our quaternary referral center in Finland, who had undetermined limb-girdle muscular dystrophy (LGMD), calf distal myopathy, or creatine kinase (CK) elevations of more than 2,000 IU/L, were selected for ANO5 genetic evaluation, and the clinical findings of patients with mutations were retrospectively analyzed. Results: A total of 25 patients with muscular dystrophy caused by 11 different recessive mutations in the ANO5 gene were identified. The …
Influence of aryl hydrocarbon- (Ah) receptor and genotoxins on DNA repair gene expression and cell survival of mouse hepatoma cells
2009
The aryl hydrocarbon receptor (AhR) mediates toxicity of a variety of environmental pollutants such as polycyclic aromatic hydrocarbons (PAHs) and dioxins. However, the underlying mechanisms and genetic programmes regulated by AhR to cause adverse effects but also to counteract poisoning are still poorly understood. Here we analysed the effects of two AhR ligands, benzo[a]pyrene (B[a]P), a DNA damaging tumour initiator and promotor and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a pure tumour promoter, on cell survival and on nucleotide excision repair (NER) gene expression. NER deals with so called "bulky" DNA adducts including those generated by enzymatically activated B[a]P. Therefore, t…
UV-B exposure causes DNA damage and changes in protein expression in northern pike (Esox lucius) posthatched embryos.
2012
The ongoing anthropogenically caused ozone depletion and climate change has increased the amount of biologically harmful UV-B radiation, which is detrimental to fish in embryonal stages. The effects of UV-B radiation on the levels and locations of DNA damage manifested as cyclobutane pyrimidine dimers (CPDs), heat shock protein 70 (HSP70) and p53 protein in newly hatched embryos of pike were examined. Pike larvae were exposed in the laboratory to current and enhanced doses of UV-B radiation. UV-B exposure caused the formation of CPDs in a fluence rate-dependent manner, and the CPDs were found deeper in the tissues with increasing fluence rates. UV-B radiation induced HSP70 in epidermis, and…
"Pamiętam, że...". O społecznej konstrukcji pamięci mieszkańców miast Ziem Zachodnich i Północnych na przykładzie Wrocławia
2017
Celem artykułu jest przedstawienie wątków pamięci społecznej, które z jednej strony opierają się na indywidualnym, potocznym doświadczeniu jednostki, z drugiej umieszczane są w społecznie wynegocjowanych ramach pamięci zbiorowej. Artykuł ten wpisuje się również w dyskusję nad miejscami pamięci i ich możliwą treścią - wykraczającą poza podtrzymujące pamięć zbiorową muzea, pomniki, upamiętniające tablice czy cmentarze - realizowaną w codziennym życiu jednostki. Przedmiotem analiz jest pamięć społeczna mieszkańców Wrocławia, ich konstrukcje światów społecznych opierające się na przestrzeni miasta obciążonej obcą kulturowo „pamięcią nieobecnych”.
„Miasto to jak mieszkanie. Nasze”. Powojenne strategie oswajania miast Ziem Zachodnich i Północnych na przykładzie Wrocławia
2018
Treść artykułu prezentuje trzy istotne zagadnienia. Pierwszym z nich jest refleksja nad praktykami zamieszkiwania (dwelling) miasta – pojęcia rozszerzającego zakres ba- dań nad przestrzenią miejską o kategorie związane z socjologią codzienności – przede wszystkim dotyczącego materialnego środowiska życia ludzi widzianego z perspektywy dyskursów niedominujących. Drugim z zagadnień jest analiza strategii oswajania ob- cego kulturowo dziedzictwa materialnego Ziem Zachodnich i Północnych, za pomocą kategorii zamieszkiwania i na przykładzie wspomnień mieszkańców Wrocławia. Trzecim aspektem są pytania stanowiące punkt wyjścia dla przyszłych projektów badawczych au- torki, postawione w wyniku prze…
Rac1-Regulated Endothelial Radiation Response Stimulates Extravasation and Metastasis That Can Be Blocked by HMG-CoA Reductase Inhibitors
2011
Radiotherapy (RT) plays a key role in cancer treatment. Although the benefit of ionizing radiation (IR) is well established, some findings raise the possibility that irradiation of the primary tumor not only triggers a killing response but also increases the metastatic potential of surviving tumor cells. Here we addressed the question of whether irradiation of normal cells outside of the primary tumor augments metastasis by stimulating the extravasation of circulating tumor cells. We show that IR exposure of human endothelial cells (EC), tumor cells (TC) or both increases TC-EC adhesion in vitro. IR-stimulated TC-EC adhesion was blocked by the HMG-CoA reductase inhibitor lovastatin. Glycyrr…
Rho GTPases in human breast tumours: expression and mutation analyses and correlation with clinical parameters
2002
In the present study, we addressed the question of a putative relevance of Rho proteins in tumour progression by analysing their expression on protein and mRNA level in breast tumours. We show that the level of RhoA, RhoB, Rac1 and Cdc42 protein is largely enhanced in all tumour samples analysed (n=15) as compared to normal tissues originating from the same individual. The same is true for 32P-ADP-ribosylation of Rho proteins which is catalysed by Clostridium botulinum exoenzyme C3. Also the amount of Rho-GDI and ERK2 as well as the level of overall 32P-GTP binding acvitity was tumour-specific elevated, yet to a lower extent than Rho proteins. Although the amount of Rho proteins was enhance…
Mapping of Biomass Residues for Potential Energy Generation from the Wine-Growing Sector in the Provinces of Palermo and Trapani
2019
This paper is based on results obtained during the project Vienergy (Interreg Italia-Malta programme 2007-2013) and contains data on the potential energy generation of biomass residues following pruning from vineyards located within the provinces of Trapani and Palermo. Analysis of the energy potential, following microgasification tests and heat production related to the biomass, included a preliminary mapping of vineyards in the area and an assessment of the total biomass available. Vineyard mapping was carried out using remote-sensing technologies: images were acquired and analysed using Landsat satellite; spectroradiometric calibration then took place using various targets obtained from …