Search results for " assembly."
showing 10 items of 283 documents
&ldquo;Out of the Can&rdquo;: A Draft Genome Assembly, Liver Transcriptome and Nutrigenomics of the European Sardine, <em>Sardina p<…
2018
Clupeiformes, such as sardines and herrings, represent an important share of worldwide fisheries. Among those, the European sardine (Sardina pilchardus, Walbaum 1792) exhibits significant commercial relevance. While the last decade showed a steady and sharp decline in capture levels, recent advances in culture husbandry represent promising research avenues. Yet, the complete absence of genomic resources from sardine imposes a severe bottleneck to understand its physiological and ecological requirements. We generated 69 Gbp of paired-end reads using Illumina HiSeq X Ten and assembled a draft genome assembly with an N50 scaffold length of 25579 bp and BUSCO completeness of 82.1% (Actinopteryg…
Optimised assembly mode reconfiguration of the 5-DOF Gantry-Tau using mixed-integer programming
2010
Pulished version of an article in the journal: Meccanica. Also available from the publisher at: http://dx.doi.org/10.1007/s11012-010-9404-y This paper presents a systematic approach based on Mixed Integer Linear Programming for finding an optimal singularity-free reconfiguration path of the 5-DOF Gantry-Tau parallel kinematic machine. The results in the paper demonstrate that singularity-free reconfiguration (change of assembly mode) of the machine is possible, which significantly increases the usable workspace. The method has been applied to a full-scale prototype and the singularity-free path has been verified both in simulations and with physical experiments using real-time control of th…
Focal adhesions and assessment of cytotoxicity
1999
Focal adhesions are highly ordered assemblies of transmembrane receptors, extracellular matrix proteins, and a large number of cytoplasmic proteins, including structural proteins, as well as tyrosine kinases, phosphatases, and their substrates. They are now accepted as a prime component of signal transduction. Because focal adhesions also play an important role in cell morphology and migration, it can be argued that their presence is indicative of healthy cells. This has been the reason for several research groups to conclude that biomaterials sustaining focal adhesion assembly are biocompatible. In this study we demonstrate that cells under cytotoxic stress may still be able to retain thei…
Subcellular localization of bacteriophage PRD1 proteins in Escherichia coli
2014
Bacteria possess an intricate internal organization resembling that of the eukaryotes. The complexity is especially prominent at the bacterial cell poles, which are also known to be the preferable sites for some bacteriophages to infect. Bacteriophage PRD1 is a well-known model serving as an ideal system to study structures and functions of icosahedral internal membrane-containing viruses. Our aim was to analyze the localization and interactions of individual PRD1 proteins in its native host Escherichia coli. This was accomplished by constructing a vector library for production of fluorescent fusion proteins. Analysis of solubility and multimericity of the fusion proteins, as well as their …
Probing the role of water in protein conformation and function
2004
Life began in a bath of water and has never escaped it. Cellular function has forced the evolution of many mechanisms ensuring that cellular water concentration has never changed significantly. To free oneself of any conceptual distinction among all small molecules, solutes and solvents, means that experiments to probe water's specific role in molecular function can be designed like any classical chemical reaction. Such an ‘osmotic stress’ strategy will be described in general and for an enzyme, hexokinase. Water behaves like a reactant that competes with glucose in binding to hexokinase, and modulates its conformational change and activity. This ‘osmotic stress’ strategy, now applied to ma…
Etude d'un emmanchement fretté compte tenu de l'effet combiné des défauts de forme et d'état de surface
2012
The main purpose of this thesis is to present two contributions in the field of shrink fitassembly taking into account the defects of form and roughness surfaces. The first contributionconcerns the study of the influence of the defect forme on the characteristics of shrinked assemblyusing an approach based on finite element modeling. We found that this defect has a significantinfluence on the resistance of the assembly. The work already done in the laboratory of mechanicsat the University of Bourgogne have been confirmed. In the second contribution, we proposed tointegrate the surface defects (roughness) by creating a homogenized finite element interface. Thistechnique has facilitated the m…
El sistema de continuidad como proceso unificador
2007
El sistema de continuidad es un procedimiento de construcción de relatos audiovisuales que tiene como fundamento la causalidad narrativa y un conjunto de normas de elaboración, especialmente en el montaje, cuya finalidad es mostrar al espectador un desarrollo argumental lógico que se desarrolla en un espacio-tiempo diegético coherente. La continuidad, está sometida a un modelo ideológico de representación que condiciona la percepción. La continuidad no es, por lo tanto, un concepto absoluto, sino que evoluciona a lo largo del tiempo. Sin embargo, el sistema de continuidad permanece vigente porque garantiza el predominio de la cadena causal y la inmersión del espectador en el universo diegét…
The focal plane assembly for the Athena X-ray Integral Field Unit instrument
2016
This paper summarizes a preliminary design concept for the focal plane assembly of the X-ray Integral Field Unit on the Athena spacecraft, an imaging microcalorimeter that will enable high spectral resolution imaging and point-source spectroscopy. The instrument's sensor array will be a ~ 3840-pixel transition edge sensor (TES) microcalorimeter array, with a frequency domain multiplexed SQUID readout system allowing this large-format sensor array to be operated within the thermal constraints of the instrument's cryogenic system. A second TES detector will be operated in close proximity to the sensor array to detect cosmic rays and secondary particles passing through the sensor array for off…
Enhanced oxidative stress and increased mitochondrial mass during Efavirenz-induced apoptosis in human hepatic cells
2010
BACKGROUND AND PURPOSE Efavirenz (EFV) is widely used in the treatment of HIV-1 infection. Though highly efficient, there is growing concern about EFV-related side effects, the molecular basis of which remains elusive. EXPERIMENTAL APPROACH In vitro studies were performed to address the effect of clinically relevant concentrations of EFV (10, 25 and 50 mu M) on human hepatic cells. KEY RESULTS Cellular proliferation and viability were reduced in a concentration-dependent manner. Analyses of the cell cycle and several cell death parameters (chromatin condensation, phosphatidylserine exteriorization, mitochondrial proapoptotic protein translocation and caspase activation) revealed that EFV tr…