Search results for " barcoding"
showing 10 items of 85 documents
Dichrorampha dinarica, new species, a century of confusion in European lepidopterology (Lepidoptera: Tortricidae) resolved by combining morphology an…
2012
Dichrorampha dinarica, new species, is described from the Dinaric mountains (Macedonia, Bosnia and Herzegovina). The newspecies was formerly confused with D. ligulana (Herrich-Schäffer, 1851) from the Alps and D. rilana Drenowsky, 1909 fromthe Rila mountains (Bulgaria). Therefore, a re-description of D. rilana is provided based on topotypical specimens. The diag-nostic morphological characters of the involved species, supported by significant divergence of the mtDNA barcode (COI fragment of 658 bp), are discussed in detail.
Application of an inexpensive and high-throughput genomic DNA extraction method for the molecular ecology of zooplanktonic diapausing eggs
2008
We describe the application of a simple, low-cost, and effective method of DNA extraction (hot sodium hydroxide and Tris, HotSHOT) to the diapausing propagules of continental aquatic invertebrates for its use in PCR amplification. We illustrate the use of the technique in cladocerans, rotifers, anostracans, notostracans, and copepod diapausing eggs. We compare the performance of the HotSHOT technique to the currently most widely used method for DNA extraction of zooplankton eggs and individuals, the chelating resin (or Chelex) technique. The HotSHOT technique overcomes several of the problems posed by Chelex and permits easy optimization for its use with 96-well plates for high-throughput D…
Confrontation of cryptic diversity and mate discrimination within Gammarus pulex and Gammarus fossarum species complexes.
2014
16 pages; International audience; Freshwater amphipods Gammarus fossarum and Gammarus pulex are widespread in Europe, with some evidence of cryptic diversity in the former. We used DNA barcoding to assess genetic diversity within and among amphipod populations and examined mate discrimination and pre-copulatory pair formation between genetically divergent individuals. Eight distinct G. fossarum and four distinct G. pulex molecular operational taxonomic units (MOTUs) were detected. Among the 33 amphipod populations sampled, 11 contained a single MOTU, 11 had two and 11 were composed of three sympatric MOTUs. Genetic divergences between sympatric MOTUs (G. fossarum and G. pulex MOTUs combined…
Recherche d'indicateurs de la résistance des sols aux maladies d'origine tellurique
2014
The Largest Subunit of RNA Polymerase II as a New Marker Gene to Study Assemblages of Arbuscular Mycorrhizal Fungi in the Field
2014
Due to the potential of arbuscular mycorrhizal fungi (AMF, Glomeromycota) to improve plant growth and soil quality, the influence of agricultural practice on their diversity continues to be an important research question. Up to now studies of community diversity in AMF have exclusively been based on nuclear ribosomal gene regions, which in AMF show high intra-organism polymorphism, seriously complicating interpretation of these data. We designed specific PCR primers for 454 sequencing of a region of the largest subunit of RNA polymerase II gene, and established a new reference dataset comprising all major AMF lineages. This gene is known to be monomorphic within fungal isolates but shows an…
First Descriptions of Larva and Pupa of
2019
In this paper, the mature larva and pupa of Bagous claudicans are described and illustrated for the first time. Measurements of younger larval instars are also given. The biology of the species is discussed in association with larval morphology and feeding habits. Overall larval and pupal morphological characters of the genus Bagous are presented. Confirmation of the larva identification as Bagous claudicans species was conducted by cytochrome oxidase I (COI) sequencing. DNA barcoding was useful for specimen identification of larval stages. The systematic position of the species within the Bagous collignensis-group, based on morphological and molecular results, is also discussed.
Assessing the use of the mitochondrial cox1 marker for use in DNA barcoding of red algae (Rhodophyta)
2011
The red algae, a remarkably diverse group of organisms, are difficult to identify using morphology alone. Following the proposal to use the mitochondrial cytochrome c oxidase subunit 1 (cox1) for DNA barcoding animals, we assessed the use of this, ne in the identification of red algae using 48 samples plus 31 sequences obtained from GenBank. The data set spanned six gene orders of red algae: the Bangiales, Ceramiales, Corallinales, Gigartinales, Gracilariales and Rhodymeniales. The results indicated that species could be discriminated. Intraspecific variation was between 0 and 4 bp over 539 bp analyzed except in Mastocarpus stellatus (0-14 bp) and Gracilaria gracilis (0-11 bp). Cryptic dive…
DNA barcoding for Arecaceae and Fabaceae species identification of two plant collections
2021
DNA barcoding is a well-known technique that enable species identification by using universal DNA sequences from a small fragment of the genome. In this work, the effectiveness of DNA barcoding approach was evaluated in order to identify different taxonomic groups among two families (Arecaceae and Fabaceae) from two flowering plant collections: some were economically important taxa from nursery production and Mediterranean plants with ornamental attitude for possible new introduction. Three chloroplast DNA barcode regions (matK, rbcL and trnH-psbA) proposed by the Consortium for the Barcode of Life (CBOL) were tested in 22 Arecaceae species and in eight Fabaceae species, including Ceratonia…
An integrated approach to the study of Hypericum occurring in Sicily
2020
An integrated approach to the study of taxa of the genus Hypericum occurring in Sicily is proposed. The results of morphological, biochemical, and molecular analyses are combined to better assess the relationships between the species investigated and test the suitability of DNA barcoding technique in the discrimination of these taxa. For the name Hypericum aegypticum subsp. webbii (Spach) N. Robson a lectotype is designated. For Hypericum triquetrifolium Turra a lectotype and a supporting epitype are designated. The presence of Hypericum perforatum L. subsp. perforatum is excluded from Sicily and the previous reports have to be referred to H. perforatum subsp. veronense (Schrank) Ces. Hyper…